intracellular Protocols

Category: Signalling Signal Transduction Protocols: Calcium Measurement Protocols

Phenomenal review of the methods and references to intracellular calcium measurements. TAKAHASHI et al Phys. Rev. 1999 - [Read Measurement of Intracellular Calcium PDF]

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Describes flow cytometric protocols using the dyes Indo-1 AM, Fluo-3, and Fura Red AM to measure intracellular calcium concentration. Support protocols detail the use of calcium buffers to calibrate a flow cytometric calcium assay, and methods to facilitate dye loading; an alternate protocol describes the use of a spectrofluorimeter to measure intracellular calcium for those investigators without access to a flow cytometer. - [Read Measurement of Intracellular Ions by Flow Cytometry Protocol]

Category: Signalling Signal Transduction Protocols: Calcium Measurement Protocols

Native Aequorin. NanoLight Technology. Aequorin has advantages over other Ca2+ indicators, for example, low leakage rate from cells, lack of intracellular compartmentalization or sequestration and it does not disrupt cell functions or embryo development. - [Read Native Aequorin]

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

A procedure for direct and indirect staining of single-cell suspensions of lymphoid tissue or peripheral blood lymphocytes to detect cell surface membrane antigens is presented. In addition, support protocols present methods for fluorescence labeling of purified antibodies. A protocol for flow cytometric analysis of intracellular antigens in single-cell suspensions is also included. - [Read Preparation of Cells and Reagents for Flow Cytometry Protocols]

Category: Signalling Signal Transduction Protocols

CHIESA et al 2001. J Biochem. Calcium-sensitive photoprotein aequorin used to analyse Ca2+ homoeostasis at the subcellular level. GFP chimaeras for studying the recruitment of protein kinase C isoforms and the activity of intracellular proteases. - [Read Recombinant aequorin and green fluorescent protein as valuable tools in the study of cell signalling]

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

INDO-1 loading and sample staining protocol for simultaneous measurement of intracellular CA2+ and cell surface antigen expression. Includes: Preparation of INDO-1; Preparation of Ionomycin. - [Read Simultaneous Measurement of Intracellular CA2+ and Cell Surface Antigen Expression Protocol]