interphase Protocols

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Fluorescence In Situ Hybridization (FISH) on Human Chromosomes and Interphase Nuclei Protocol - http://users.ugent.be/~fspelema/neubla/protocol/fish.htm

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for fluorescence in situ hybridization (FISH) on human chromosomes and interphase nuclei. Includes: Plasmid probes; COSMID-, YAC- and LIBRARY PROBES; Probe detection. - [Read Fluorescence In Situ Hybridization (FISH) on Human Chromosomes and Interphase Nuclei Protocol]

Hybridisation of Interphase Nuclei Protocol - http://www.sanger.ac.uk/HGP/methods/cytogenetics/interphase_hybridisation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Hybridisation of Interphase Nuclei Method. Sanger Institute. - [Read Hybridisation of Interphase Nuclei Protocol]

In Situ Methods to Localize Transgenes and Transcripts in Interphase Nuclei - http://www.plantmethods.com/content/2/1/18

Category: Plant Biology Protocols: Plant Genetics Protocols

To accurately predict the activity of a transgene it is critical to understand its location and dynamics in the 3-D interphase nucleus. Developed in situ methods to visualize transgenes (including single copy genes) & their transcripts during interphase from different tissues & plant species. These techniques reduce the time necessary for characterization of transgene integration by eliminating the need for time-consuming segregation analysis and extend characterization to the interphase nucleus - [Read In Situ Methods to Localize Transgenes and Transcripts in Interphase Nuclei]

In Situ Methods to Localize Transgenes and Transcripts in Interphase Nuclei - http://www.plantmethods.com/content/2/1/18

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

In situ methods to visualize transgenes (including single copy genes) and their transcripts during interphase from different tissues and plant species. These techniques reduce the time necessary for characterization of transgene integration by eliminating the need for time-consuming segregation analysis, and extend characterization to the interphase nucleus, thus increasing the likelihood of accurate prediction of transgene activity. - [Read In Situ Methods to Localize Transgenes and Transcripts in Interphase Nuclei]

Interphase nuclei - http://www.sanger.ac.uk/HGP/methods/cytogenetics/interphase_nuclei.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Interphase nuclei preparation for FISH. Sanger Institute. - [Read Interphase nuclei]

Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique - http://www.riedlab.nci.nih.gov/publications/2408.2950_Isolat_Interph_Nuc4Hed204.pdf

Category: Cell Organelle Protocols: Nucleus Protocols

Protocol for the isolation of interphase nuclei from Paraffin Section for FISH using the Hedley Technique. - [Read Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique]

Purification of Demethylated Sphingomyelin Protocol - http://www.duke.edu/web/ceramide/protocols/0032.html

Category: Lipid Protocols

Protocol for the purificiation of demethylated sphingomyelin. Includes: Lower, chloroform phase; Upper, aqueous phase and interphase. - [Read Purification of Demethylated Sphingomyelin Protocol]

Small Marker Chromosome Identification in Metaphase and Interphase using Centromeric Multiplex FISH - http://info.med.yale.edu/genetics/ward/tavi/CMFISH.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for small marker chromosome identification in metaphase and interphase using centromeric multiplex FISH (CM-FISH). This approach allows the identification of marker chromosomes and other aneuploides on one single cytogenetic slide, in less than two hours. - [Read Small Marker Chromosome Identification in Metaphase and Interphase using Centromeric Multiplex FISH]

Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_220-228.pdf

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for whole mount fluorescence in situ hybridization (FISH) of repetitive DNA sequences on interphase nuclei of the small cruciferous plant Arabidopsis thaliana. Includes: Seed sterilization and germination; Tissue fixation; Labeling of the probe DNA; Pretreatment; In situ hybridization; Pre-absorption of antibodies; Posthybridization washes; Immunocytochemical detection; Direct detection; Indirect detection; Staining and mounting; Fluorescence microscopy. - [Read Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase]

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Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.