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Quantification of Apoptosis and Cell Cycle Distribution of Primary B Cells Using Propidium Iodide - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000024.pdf?pid=PP00000024

Category: Immunology: B Cell Protocols

The water soluble, DNA intercalator, propidium iodide (PI), is used to bind to DNA after permeabilization of cells with NP40. The amount of dye bound correlates with the content of DNA within a given cell. Once cells are stained, they are analyzed on a flow cytometer. The relative content of DNA indicates the distribution of a population of cells throughout the cell cycle. - [Read Quantification of Apoptosis and Cell Cycle Distribution of Primary B Cells Using Propidium Iodide]

Quantification of Apoptosis and the Cell Cycle Distribution of Primary B Cells Using PI - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000024.pdf?pid=PP00000024

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

The water soluble, DNA intercalator, propidium iodide (PI), is used to bind to DNA after permeabilization of cells with NP40. The amount of dye bound correlates with the content of DNA within a given cell. Once cells are stained, they are analyzed on a flow cytometer. The relative content of DNA indicates the distribution of a population of cells throughout the cell cycle. - [Read Quantification of Apoptosis and the Cell Cycle Distribution of Primary B Cells Using PI]