insulin Protocols

Category: Cell Culture Protocols

Protocol used to study secretion of proteins and prostaglandins by endometrium from the cow, ewe, mare, bitch and other species. The technique is also useful for culture of peri-implantation conceptuses and placental tissues for metabolic labelling studies and to obtain conceptus secretory proteins for biological studies.The medium used is called Pig MEM, which is a modified minimum essential medium supplemented with non-essential amino acids, vitamins, insulin and additional glucose. - [Read Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion]

Category: Mouse Protocols

Mice fed with the cytohesin inhibitor SecinH3 for two days develop hepatic insulin resistance that can be identified by reduced liver glycogen levels, increased serum insulin and ketone body levels and decreased serum non-esterified fatty acid. To confirm the presence and identity of SecinH3 in mouse liver, we extracted the compound from liver homogenates with chloroform and identified it by LC/MS. - [Read Extraction of the SecinH3 from Mouse Liver Protocol]

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Purification of the Adipocyte Insulin Receptor by Immunoaffinity Chromatography. - [Read Purification of the Adipocyte Insulin Receptor by Immunoaffinity Chromatography]

Category: PCR Protocols: Real-Time PCR Protocols

Protocol for suppression of insulin target gene expression by SecinH3 quantified by real-time PCR. - [Read Suppression of Insulin Target Gene Expression by SecinH3 Quantified by Real-Time PCR Protocol]