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Aseptic Technique for Cell Culture Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B7EFFEB261151E9039B66FD5981&objectid=66739FA0D0CABBF1C466C9F466ABDF9A

Category: Cell Culture Protocols: Sterile Technique Protocols

This unit describes some of the ways that a laboratory can deal with the constant threat of microbial contamination in cell cultures. A protocol on aseptic technique is described first. This catch-all term universally appears in any set of instructions pertaining to procedures in which noncontaminating conditions must be maintained. - [Read Aseptic Technique for Cell Culture Protocol]

Deltavision Deconvolution Instructions - http://depts.washington.edu/keck/dv/start.html

Category: Molecular Imaging

Deltavision Deconvolution Instructions. Keck Imaging Center. - [Read Deltavision Deconvolution Instructions]

Excellent Centrifuge Safety Use Manual PDF - http://www.hse.ubc.ca/resource/files/CentrifugeSafetyManual.pdf

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Excellent Centrifuge Safety Use Manual. 30 pages. Training, Use, Maintenance, Records and Responsibility, Mechanical Breakdown, Emergency Procedure, Specific Safety Instructions Beckman Sorvall and Eppendorf. HSE UBC. - [Read Excellent Centrifuge Safety Use Manual PDF]

Experiments on Parochial Altruism in Humans; Procedures and Instructions - http://www.natureprotocols.com/2006/08/24/experiments_on_parochial_altru.php

Category: Developmental Biology: Utilizing Model Organisms Protocols

In the experiment there are three players A, B, and C. Player A gets an initial endowment of 10, player B gets 0 and player C gets 5 points. First, player A decides how many of his 10 points to transfer to B. Then, player C observes the decisions of A and gets the possibility to leave the payoffs unaffected or to punish A by deducting 3 (or 6) points from A at a cost of 1 point (or 2 points) for C.... - [Read Experiments on Parochial Altruism in Humans; Procedures and Instructions]

FPLC Protocol - http://users.path.ox.ac.uk/~ciu/Methods/Protein/fplc.html

Category: Chromatography Protocols

FPLC Protocol. The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting. For use of individual columns follow the "instructions" (in the green folder) which accompany each one. Sir William Dunn School of Pathology, Oxford University. - [Read FPLC Protocol]

Instructions for Using Anaerobic Chamber and Gassing Station - http://rothlab.ucdavis.edu/protocols/anaerobic-techniques.html

Category: Bacterial Protocols

Instructions for using anaerobic chamber and gassing station. - [Read Instructions for Using Anaerobic Chamber and Gassing Station]

Instructions for Using Molecular Devices LMax Luminometer - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/II.%20Eukaryotes/F.%20Luciferase%20Assays/1.%20Luminometer%20operation.pdf

Category: Reporter Gene Assays: Luciferase Assay Protocols

Instructions for using molecular devices LMax Luminometer. - [Read Instructions for Using Molecular Devices LMax Luminometer]

Leica Confocal Instructions - http://depts.washington.edu/keck/leica/instructions.htm

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Leica Confocal Instructions. Keck Imaging Center. - [Read Leica Confocal Instructions]

Leica SL Confocal Microscopy Imaging Instructions - http://depts.washington.edu/keck/LeicaSL/SL%20Instructions.htm

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Leica SL Confocal Microscopy Imaging Instructions. Keck Imaging Center. - [Read Leica SL Confocal Microscopy Imaging Instructions]

Microsome Purification from Rat Liver Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p2051

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols: Microsome Isolation Protocols

This protocol gives instructions for preparing microsomes from a rat liver. - [Read Microsome Purification from Rat Liver Protocol]

Processing Adherent Cultured Cells for Tissue Microarray Controls ... - http://www.tissuearray.org/CellLinesProtocolforTMA112.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Cell Fixation. 1. Gently wash cells 2x in sterile 1XPBS, ... [Take to Research Histology for embedding] Instructions: Embed pellets in vertical ... Jena Giltnane Yale University Dept. of Pathology. Processing Adherent Cultured Cells for Tissue Microarray Controls. - [Read Processing Adherent Cultured Cells for Tissue Microarray Controls ...]

Riboprobe In Vitro Transcription Systems - http://www.promega.com/tbs/tm016/tm016.pdf

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

In vitro transcription systems includes instructions for use of products P1420, P1430, P1440, P1450 and P1460.Includes information and protocols on RNA Transcription in vitro. Information on DNA Template Preparation;Synthesis of High-Specific-Activity Radio labeled RNA Probes;Determining Percent Incorporation and Probe Specific Activity;Removal of the DNA Template Following Transcription;Removal of unincorporated nucleotides;Synthesis of large amounts of RNA;Capping RNA for in vitro translation. - [Read Riboprobe In Vitro Transcription Systems]

Transient Transfection: Viral Production - http://genetics.med.harvard.edu/~cepko/protocol/mike/V1.html

Category: Transfection Protocols: Transient Transfection Protocols

This transient transfection protocol for the ecotropic/amphotropic producer line includes instructions for titering and concentration of viral stocks. This procedure gives consistently higher transfection efficiency. - [Read Transient Transfection: Viral Production]

Widefield Microscopy Nikon Upright with Operating Instructions - http://depts.washington.edu/keck/Widefield/equipment.htm

Category: Molecular Imaging

Widefield Microscopy Nikon Upright with Operating Instructions. Keck Imaging Center. - [Read Widefield Microscopy Nikon Upright with Operating Instructions]

Xenopus Fluorescent in situs and FCIS Protocol - http://www.xenbase.org/other/static/methods/FISH.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocols for performing wholemount fluorescent in situs. Instructions for two-color FISH and for a combination of fluorescent and colorimetric in situs we have called FCIS. Data can be imaged with a fluorescence stereoscope or a confocal. - [Read Xenopus Fluorescent in situs and FCIS Protocol]

Zeiss Meta Confocal Microscopy Imaging Instructions - http://depts.washington.edu/keck/Zeiss/Zeiss%20Instructions.html

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Zeiss Meta Confocal Microscopy Imaging Instructions. Operating Instructions with photos included. Keck Imaging Center. - [Read Zeiss Meta Confocal Microscopy Imaging Instructions]

Articles (1-10 of 10)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Acid Washing Microinjection Pipettes Protocol

Acid Washing of Glass microinjection pipettes protocol.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.