insertions Protocols

Category: PCR Protocols: Inverse PCR Protocols

Protocol for inverse PCR & cycle sequencing of P element insertions for STS generation. - [Read Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation Protocol]

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

A powerful way to identify a mutation in the gene of interest and to test mutant plants for phenotypes that are predicted to result from loss of function of that gene is by PCR screening. Pools of insertion lines are screened using one primer corresponding to the gene of interest and one primer corresponding to the end of the insertion element. The synthesis of a product indicates the presence of an insertion in the gene of interest. - [Read Screening DNA Pools for T-DNA Insertions in Arabidopsis Genes Protocol]