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Gene Delivery by Direct Injection (Microinjection) Using a Pulsed-Flow System Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4653

Category: Microinjection Protocols: Microinjection of DNA Protocols

This protocol describes a method for pulsed-flow microinjection using the Eppendorf FemtoJet injector and Eppendorf InjectMan; this is the most common type of pulsed-flow microinjection system currently being used. The advantage of this type of system over a controlled-flow system is that much more control is available over the injection parameters, reducing variability in injections. In addition, the system allows a diagonal insertion of the needle into the cell. - [Read Gene Delivery by Direct Injection (Microinjection) Using a Pulsed-Flow System Protocol]

Making and Injecting Xenopus Embryos Protocol - http://spot.colorado.edu/~klym/Methods/embryos.htm

Category: Xenopus Protocols: Xenopus Injection Protocols

Protocol for making and injecting Xenopus embryos. Includes: Injections; Egg Activation and Inversion Experiments; - [Read Making and Injecting Xenopus Embryos Protocol]

Microinjection of dsRNA into Drosophila Embryos Protocol - http://www.cshprotocols.org/cgi/content/full/2006/21/pdb.prot4516

Category: Microinjection Protocols: Microinjection of DNA Protocols

This protocol provides a description of how to introduce double-stranded RNA (dsRNA) into Drosophila embryos by microinjection. Several days of preparation are required before injections into Drosophila embryos begin. Flies must be in abundant supply for egg collection. Bombardment of embryos with dsRNA-coated gold particles (Delivery of dsRNA into Drosophila Embryos by a Gene Gun) can be used as an alternative. - [Read Microinjection of dsRNA into Drosophila Embryos Protocol]

Xenopus Egg CSF Extract Preparation Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1049

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol describes the preparation of Cytostatic Factor (CSF) from frog eggs. CSF can be used in in vitro spindle assembly assays. Includes: Frog injections; Preparation Before Starting the Extract Preparation; Extract Preparation. - [Read Xenopus Egg CSF Extract Preparation Protocol]

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Coupling Cysteine Containing Peptides to Carrier Protein for Immunization and Polyclonal Antibody

This protocol is used in the production of polyclonal antibodies that recognize a peptide sequence of interest.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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