inhibition Protocols

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Allium Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/8.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

The Allium test provides a rapid screening procedure for chemicals, pollutants contaminants, etc. which may represent environmental hazards. Root growth inhibition and adverse effects upon chromosomes provide an indication of likely toxicity. - [Read Allium Test]

Allium Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/8.htm

Category: Plant Biology Protocols

ELISA Inhibition Assay - http://wheat.pw.usda.gov/~lazo/methods/goldberg/elisa.html

Category: ELISA Protocols

Protocol and method for ELISA Inhibition Assay. Michael Coyne. Goldberg Lab - [Read ELISA Inhibition Assay]

ELISA Method Measure Inhibition COX Enzymes - http://www.natureprotocols.com/2006/11/22/an_elisa_method_to_measure_inh.php

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol describes a procedure measuring cyclooxygenase activity by quantifying PGE2 produced by enzymatic conversion of arachidonic acid, in the presence or absence of potential inhibitors. This high-throughput method has the advantage that it directly measures cyclooxygenase activity and requires little enzyme. - [Read ELISA Method Measure Inhibition COX Enzymes]

ELISA Method to Measure Inhibition of the COX Enzymes Protocol - http://www.natureprotocols.com/2006/11/22/an_elisa_method_to_measure_inh.php

Category: ELISA Protocols

The cyclooxygenase (COX) reaction can be monitored by measurement of oxygen consumption, peroxidase co-substrate oxidation or prostaglandin (PG) detection. This protocol describes a procedure measuring cyclooxygenase activity by quantifying PGE2 produced by enzymatic conversion of arachidonic acid, in the presence or absence of potential inhibitors. - [Read ELISA Method to Measure Inhibition of the COX Enzymes Protocol]

EMBRYONIC STEM CELL TEST (EST) - http://ecvam-sis.jrc.it/IP1685/published/indexed_113.html

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: Embryotoxic Potential Testing of Chemicals

EMBRYONIC STEM CELL TEST (EST). The embryotoxic potential of chemicals is determined by the evaluation of the inhibition of differentiation of embryonic stem cells (ES) and the inhibition of growth of ES and 3T3 cells. Scientific Information Service - [Read EMBRYONIC STEM CELL TEST (EST)]

GTPÎ³S-induced Inhibition of Binding - http://www.hopkinsmedicine.org/cellbio/devreotes/gtp.htm

Category: Immunology: Chemotaxis

GTPÎ³S-induced Inhibition of Binding. Chemotaxis assay protocols. Devreotes Lab, John Hopkins Medical Centre. - [Read GTPÎ³S-induced Inhibition of Binding]

GTPÎ³S-Induced Inhibition of cAMP Binding to the cAMP Receptor (cAR1) in Dictyostelium Discoideum - http://www.bio.com/protocolstools/protocol.jhtml?id=p1175

Category: Signalling Signal Transduction Protocols

This protocol assays inhibition of in vivo binding of [3H]-cAMP to cAR1 by GTPÎ³S. Dictyostelium discoideum respond to extracellular cAMP through the cAMP chemoattractant receptor (cAR1). Binding of cAMP to the G protein-coupled cAR1 is inhibited by the GTP analog GTPÎ³S. Protocol includes information on: Solutions used, BioReagents and Chemicals and Protocol Hints. - [Read GTPÎ³S-Induced Inhibition of cAMP Binding to the cAMP Receptor (cAR1) in Dictyostelium Discoideum]

Human Oesophageal Culture Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/28.htm#TOP

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This procedure describes a method for establishing short-term explant cultures of oesophageal mucosa. Adverse effects produced by exposure to radiation or test compounds can be detected as an inhibition of cell outgrowth. - [Read Human Oesophageal Culture Protocol]

Human Oesophageal Culture Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/28.htm#TOP

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Protocol describes a method for establishing short-term explant cultures of oesophageal mucosa. Adverse effects produced by exposure to radiation or test compounds can be detected as an inhibition of cell outgrowth. - [Read Human Oesophageal Culture Protocol]

Human Skin Fibroblast/Collagen Lattice Cytotoxicity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/27.htm#TOP

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

Skin fibroblasts are incorporated into 3-D collagen lattices containing the test compounds. An inhibition of lattice contraction indicates a possible toxic effect which is verified by trypan blue exclusion for cell viability. - [Read Human Skin Fibroblast/Collagen Lattice Cytotoxicity Test]

Isolation and Freezing of Primary Mouse Embryonic Fibroblasts (MEF) For Feeder Plates - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4482

Category: Stem Cell Protocols: Feeder Cell Preparation Protocols

This protocol describes the isolation of fibroblasts from mouse embryos. Mouse embryonic fibroblast (MEF) cells are used as a feeder layer for the culture of mouse embryonic stem (ES) cells to help maintain them as pluripotent stem cells. The inhibition of ES-cell differentiation provided by the MEF feeders appears to be due to their production of leukemia inhibitory factor (LIF). - [Read Isolation and Freezing of Primary Mouse Embryonic Fibroblasts (MEF) For Feeder Plates]

The Frame Cytotoxicity Test Kenacid Blue - http://embryo.ib.amwaw.edu.pl/invittox/prot/15.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

The cytotoxic effect of chemicals upon cells in culture is measured by the change in total cell protein arising from the inhibition of cell proliferation (Kenacid Blue R dye binding method). - [Read The Frame Cytotoxicity Test Kenacid Blue]