information Protocols

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use the mtr gene as a selectable marker for loss or gain of function. - [Read How to Use the mtr Gene as a Selectable Marker for Loss or Gain of Function]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use UV for mutagenesis. - [Read How to Use UV for Mutagenesis]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to wash agar. - [Read How to Wash Agar]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to work with slime and other cell wall-deficient strains. - [Read How to Work with Slime and Other Cell Wall-Deficient Strains]

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocols and information on general HPLC column care. Includes: Silica based columns and Polymer based columns.  Protocols included: pH stability, Mechanical stability, Mobile phases (Eluents), Proper storage of HPLC columns, Equilibration time, Regeneration of a column, Regeneration of RP packings, Regeneration of NP (Normal Phase) packings, Regeneration of Ion Exchange Packings - [Read HPLC Column Care Protocols and Information]

Category: Microscopy Protocols

The atomic force microscope (AFM) is one of the most powerful tools for determining the surface topography of native biomolecules at subnanometer resolution. The AFM can also provide insight into the binding properties of biological systems. In order to determine the specific interaction between two kinds of molecules (e.g., avidin and biotin). Includes information on principle of AFM and application of AFM. - [Read Imaging, Measuring and Manipulating Native Biomolecular Systems with the Atomic Force Microscope]

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Information on Immortalized Mammary Epithelial Cells (IMEC). Includes: Background Information on IMEC; Culturing; Trypsinization. - [Read IMEC Maintenance]

Category: Immunology: Immunofluorescence Protocols: Immunofluorescence Staining Protocols

Provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated. The binding of these molecules is detected by incubating the sample with a secondary antibody specific for immunoglobulin molecules and conjugated to fluorophore. - [Read Immunofluorescence Staining Protocol]

Category: Protein Protocols: Immunoprecipitation Protocols

Immunoprecipitation Protocol and Information. IP. Cell Lysate Preparation, preclearing, Immunoprecipitation Buffers. Ebioscience. - [Read Immunoprecipitation Protocol and Information]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

With this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are used. Includes information on: Permeabilization of Cells; In vitro Transcription Reaction (Run-off); Isolation of RNA; Preparation of Slot Blot Membrane for Hybridization; Hybridization of Nitrocellulose Membrane; TCA Precipitation to Determine Incorporation of [32P] GTP into Nucleic Acid - [Read In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription. Includes information on: Hot transcription reactions, notes and troubleshooting. - [Read In Vitro Transcription Protocol]

Category: Microscopy Protocols: Confocal Microscopy Protocols

Information on: Applications of Confocal Microscopy; Practical Instruments; Limitations of point-scanning Confocal Microscopy; Parallel beam confocal Imaging Systems. - [Read Information on Confocal Imaging]

Category: Peptide Protocols: Antibodies from Peptides Protocols

Great resource for information. on making antibodies against peptides - Pierce - [Read Information to Make Antibodies against Peptides - Pierce]

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describing an JNK Kinase Assay. Protocol includes information on reaction buffer. - [Read JNK Kinase Assay]

Category: Affiliates

Kill Bed Bugs. Information on how to treat, control and manage bedbug infestations. - [Read Kill Bed Bugs]

Category: General Research Protocols and Methods: Laboratory Safety

Laboratory Safety Manual. McGill EHS. Workplace Hazardous Materials Information System (WHMIS), Control of Chemical Hazards, Handling and Storage of Laboratory Chemicals, Fire Safety in the Laboratory, Hazardous Waste Disposal. - [Read Laboratory Safety Manual]

Category: General Research Protocols and Methods: Laboratory Safety

Laboratory Safety Program - Emergency Preparedness, Autoclave Safety, Biosafety, Carcinogens, Chemical Hygiene Plan, Glass Disposal, Fire Safety, Eyewash and Safety Shower Information, Fume Hood Procedures and Practices. University of Wisconsin - Milwaukee. - [Read Laboratory Safety Program]

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

Information on lambda methods media and solutions. - [Read Lambda Methods Media and Solutions]

Category: Cell Culture Protocols

Information on the methods used to cultivate large amounts of cells for the purpose of obtaining an endogenous or recombinant product. - [Read Large-Scale Cell Culture Protocol]

Category: Chromatography Protocols: Gas Chromatography Protocols

Linkage analysis provides information on sugar type, ring size, and substitution positions for each monosaccharide.  The method in this protocol, using NaOH as the base, is one of the simpler linkage analysis methods.  It requires approximately 1-5 µg of carbohydrate. - [Read Linkage Analysis Using the NaOH Methylation Method Protocol]

Category: Microscopy Protocols: In Vivo Imaging Protocols

Live nematodes can be studied at all levels of microscopic resolution. Data collection includes information on: Photographic Records, Video Microscopy,Multiple-Focal-Plane Time-Lapse Recording Systems and Digital Imaging. - [Read Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection]

Category: Microscopy Protocols: Confocal Microscopy Protocols

Confocal laser scanning microscopy (CLSM) is a relatively new light microscopical imaging technique which has found wide applications in the biological sciences. The primary value of the CLSM to the biologist is its ability to produce optical sections through a 3-D specimen-e.g., an entire cell or a piece of tissue - that, to a good approximation, contain information from only one focal plane. Article includes principle and applications of confocal laser scanning microscope. - [Read Looking Inside Cells and Tissues by Optical Sectioning with a Confocal Laser Scanning Microscope]

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

LRSM Confocal Microscope Home Page. Information on use and how confocal microscopy works. - [Read LRSM Confocal Microscope Home Page]

Category: Protein Microarray Protocols

Lysate Microarrays. High throughput methods that detect abundance and post-translational modification. MacBeath Lab - [Read Lysate Microarrays - Information]

Category: Mass Spectrometry Protocols

Protein sequencing by mass spectrometry. Mass analysis of peptides and proteins. Identification of modifications in proteins. Good information.Univ. Virginia. Dr. Nicholas Sherman - [Read Mass spectrometry analysis and identification of proteins]