information Protocols

Category: Bacterial Protocols

Culture protocols and information for T brucei. Detailed guide to the practice and history ofTrypanosoma brucei cultivation. This covers the culture of bloodstream and procyclic forms in liquid media and on agarose plates. - [Read Culture Protocols and Information for T brucei]

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Protocol describing cytochrome oxidase manometric analysis. Protocol includes: Manometric Analysis and information on the the Gilson Constant Pressure Respirometer. - [Read Cytochrome Oxidase Manometric Analysis]

Category: RNAi Technology Protocols

Information on how detect and quantitate MicroRNA in laser capture microdissection samples. Includes: Obtain Laser Capture Microdissected Samples; Isolate miRNA from LCM Samples; Quantitate miRNA by qRT-PCR; Detection of miRNA in Microdissected Tissue from Mouse Brain by qRT-PCR; Differential Expression of MicroRNA in Whole Brain Tissue Compared to a More Homogeneous Population of Cells. - [Read Detect and Quantitate MicroRNA in Laser Capture Microdissection Samples]

Category: Mouse Protocols: Mouse Embryology Protocols

Information on the detection of estrus in mice. - [Read Detection of Estrus in Mice]

Category: Fungi Protocols: Fungi Genetics Protocols

Standard operating procedure for the determination of tissue fungal burden utilizing quantitative real time polymerase chain reaction (QPCR). This standard operating procedure will provide information on how to assess fungal tissue burden of infected animals by use of a single copy (FKS) or multicopy gene (18s RNA) to assess the number of fungal cell nuclei present. - [Read Determination of Tissue Fungal Burden Utilizing Quantitative Real Time Polymerase Chain Reaction]

Category: Xenopus Protocols

Information on the diseases of Xenopus tropicalis. Includes: PREMETAMORPHS: Bacterial Infection of Embryos; Protozoal Infestation of Tadpoles; POSTMETAMORPHS: Mycobacteriosis; Chytridiomycosis (Batrachochytrium dendrobatidis). - [Read Diseases of Xenopus tropicalis]

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: Embryotoxic Potential Testing of Chemicals

EMBRYONIC STEM CELL TEST (EST). The embryotoxic potential of chemicals is determined by the evaluation of the inhibition of differentiation of embryonic stem cells (ES) and the inhibition of growth of ES and 3T3 cells. Scientific Information Service - [Read EMBRYONIC STEM CELL TEST (EST)]

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Information for EMSA (Gel Shift) technique. Includes: Critical EMSA Reaction Parameters. - [Read EMSA (Gel Shift) Technique Information]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Provides information on specific parameters you need to be aware of when using eukaryotic in vitro translation systems. Includes: DNA Template Considerations; Protein Labeling; Non-Radioactive Protein Labeling. - [Read Eukaryotic In Vitro Translation Systems]

Category: Signalling Signal Transduction Protocols

This kinase assay is meant to determine whether an agonist or event can influence the autophosphorylation of FAK. The addition of 1 μl of polyGT to the kinase reaction mix will determine the activity of the enzyme against a substrate. Includes information on: Harvest, Immunoprecipitation, Kinase Reaction and Antibody Detection of FAK. - [Read FAK Autophosphorylation Assay]

Category: Chromatography Protocols: Gel Filtration Chromatography Protocols

Gel Filtration Column Chromatography Protocol. Includes information on: Calibrating the column, Applying standards to the column, Running SDS-PAGE Gels (Tris-Tricine “Schägger” Gels) and includes questions. North Arizona University. - [Read Gel Filtration Column Chromatography]

Category: Signalling Signal Transduction Protocols

This protocol assays inhibition of in vivo binding of [3H]-cAMP to cAR1 by GTPγS. Dictyostelium discoideum respond to extracellular cAMP through the cAMP chemoattractant receptor (cAR1). Binding of cAMP to the G protein-coupled cAR1 is inhibited by the GTP analog GTPγS. Protocol includes information on: Solutions used, BioReagents and Chemicals and Protocol Hints. - [Read GTPγS-Induced Inhibition of cAMP Binding to the cAMP Receptor (cAR1) in Dictyostelium Discoideum]

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Hairpin-bisulfite PCR will be a powerful tool in studying other processes for which genetic or epigenetic information differs on the two complementary strands of DNA. Laird et al., 2003. PNAS - [Read Hairpin-bisulfite PCR: Assessing epigenetic]

Category: Signalling Signal Transduction Protocols

This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography. Includes information: H1 Kinase Assay on Individual Xenopus Oocytes; H1 Kinase Assay on Xenopus Egg Extract Samples; H1 Kinase Assay on Tissue Culture Cells; Helpful protocol hints. - [Read Histone H1 Kinase Activity Assay]

Category: Immunohistochemistry Protocols: Fixation Protocols

Information on histotechniques. Includes: Fixation - types of fixatives; factors affecting fixation; General usage of fixatives; Tissue Processing; Sectioning; Frozen Sections; Staining; H and E staining; Coverslipping; Decalcification; Artefacts in Histologic Sections; Problems in Tissue Processing. - [Read Histotechniques Fixation]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how and where to obtain information on Neurospora. - [Read How and Where to Obtain Information on Neurospora]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to activate ascospores and optimize the recovery of progeny. - [Read How to Activate Ascospores and Optimize the Recovery of Progeny]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to amplify DNA from macroconidia. - [Read How to Amplify DNA from Macroconidia]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to assay light responses in Neurospora. - [Read How to Assay Light Responses in Neurospora]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on How to avoid contamination by airborne conidia. - [Read How to Avoid Contamination by Airborne Conidia]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres. - [Read How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to certify that Neurospora is nonhazardous. - [Read How to Certify that Neurospora is Nonhazardous]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to choose and prepare media. - [Read How to Choose and Prepare Media]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to choose wild type laboratory strains of N. crassa for reference and for special uses. - [Read How to Choose Wild Type Laboratory Strains of N. crassa for Reference and for Special Uses]

Category: Fungi Protocols: Neurospora Protocols

Information on how to color-code media. - [Read How to Color-Code Media]