information Protocols

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describes the activation of MAP Kinase activity. Protocol contains information on: Buffers: ST, STE, Lysis Buffer Buffer A, Buffer B and 10x Kinase Buffer. - [Read Activation of p70s6k and MAP Kinases: IP and Kinase Activity]

Category: Real Time PCR

Affymetrix Real Time PCR information and optimization. Yale U. Keck Foundation. - [Read Affymetrix Real Time PCR information and optimization]

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Aggregations - Production of Chimerae

Aggregation: Production of Chimerae. Detailed ES cell growth and preparation information for aggregations. Edward W. Scott Univ. Florida Shands Cancer Center - [Read Aggregation: Production of Chimerae]

Category: Signalling Signal Transduction Protocols

Technique for Alkaline Phosphatase (APAAP). Techniques includes information on: Cytological Preparations, Fixation, Technique, Results, Substrate Solution and 0.005M Tris/HC1 Saline Buffer pH 7.6. - [Read Alkaline Phosphatase (APAAP) Technique]

Category: Protein Protocols: Immunoprecipitation Protocols

Antibody-antigen complexes are removed from solution by addition of an insoluble form of an antibody binding protein such as Protein A, Protein G or second antibody. Immunoprecipitation protocols / methodology and technical background information. P.J. Ha - [Read Analysis of Proteins by Immunoprecipitation]

Category: Microarray Protocols: Microarray Analysis Protocols

Information on anatomy of a comparative gene expression study.  Includes: Choosing cell populations; mRNA Extraction and Reverse Transcription; Fluorescent labeling of cDNA's; Hybridization of DNA microarray; Scanning the hybridized array; Interpreting the scanned image. - [Read Anatomy of a Comparative Gene Expression Study]

Category: Antibody Microarray Protocols

Antibody Array Production and Data Analysis. Probing Antigen Arrays and washing/ Blocking. Great Protocols and information. Brian Kidd. Stanford. - [Read Antibody Array Production and Analysis]

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Apoptosis Methods Movies and Information. Immuteach. - [Read Apoptosis Methods Movies and Information]

Category: Genetics and Genomics: Cancer Genetics Protocols

SKY has also been applied for the mouse genome, enabling investigators to extrapolate information from mouse models of cancer to their human counterparts. This review will address the advances that SKY has facilitated in the field of cancer cytogenetics, as well as its variety of application in the cancer research laboratories. - [Read Applications of SKY in Cancer Cytogenetics Review]

Category: Signalling Signal Transduction Protocols

Protocol allows you to measure the content of cyclic adenosine 3',5'-monophosphate (cyclic AMP or cAMP) in splenic B lymphocytes (B cells) in an enzyme-linked immunoassay. This protocol utilizes acetylation of cAMP to improve sensitivity and reduce interference. Protocol includes information on: how to determine cAMP, calculations and reagents and materials. - [Read Assay of Cyclic AMP in Lysates of Cells]

Category: BACs Bacterial Artificial Chromosome Protocols

Information for BACs, Bacterial Artificial Chromosomes. Includes: Existing vectors; pBAC108L; pBeloBAC11; pBACe3.6; pFW11 and F' factor; pCC1BAC; pSMART VC Vectors; pIndigoBAC-5; Cosmids; pWEB-TNCTM; SuperCos 1; pFos1; PAC vectors; BAC purification; Putting the BAC into E. coli. - [Read BACs Bacterial Artificial Chromosomes]

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

The ability to synthesize RNA in the lab is critical to many techniques.Radiolabeled and nonisotopically labeled RNA probes, generated in small scale transcription reactions can be used in blot hybridizations and nuclease protection assays. This article includes information on: Requirements For Transcription, RNA Phage Polymerases, Template Options: Plasmids, PCR Products, Oligonuclotides and cDNA, Sense or Antisense, Conventional Or Large Scale Synthesis, Products for In Vitro Transcription. - [Read Basic Information on In Vitro Transcription]

Category: Plant Biology Protocols: Plant Proteins, Peptides and Antigens

BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science. - [Read Blue-Native PAGE in Plants: A Tool in Analysis of Protein-Protein Interactions]

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

A detailed and well thought out Bradford protein assay page using a spectrophotometer. Includes information such as the Bradford assay is very fast and uses about the same amount of protein as the Lowry assay, comments, procedural steps, equipment used and more. - [Read Bradford Protein Assay]

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Bradford Protein Assay Spectrophotometry.  Includes spectrophotometry information and the Bradford protein assay: A spectrophotometer or colorimeter makes use of the transmission of light through a solution to determine the concentration of a solute within the solution.  A spectrophtometer differs from a colorimeter in the manner in which light is separated into its component wavelengths.  A spectrophotometer uses a prism to separate light and a colorimeter uses filters. - [Read Bradford Protein Assay Spectrophotometry]

Category: Mouse Protocols: Mouse Embryology Protocols

Information and protocol for breeding mice. - [Read Breeding Mice Protocol]

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol and information related to C. elegans integrating extrachromasomal arrays. - [Read C. elegans Integrating Extrachromasomal Arrays Protocol]

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Information on how to quantitatively assess DNA content. Includes: Propidium iodide staining protocol and staining of DNA by Mithramicin Protocol - [Read Cell Cycle Analysis - Propidium Iodide]

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Information on propidium iodide for cell cycle analysis. - [Read Cell Cycle Analysis - Propidium Iodide]

Category: Cell Immortalization Protocols

Information why cell immortalization is important. Also the available tools to be able to perform cell immortalization. - [Read Cell Immortalization]

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Choosing a cell viability or cytotoxicity assay from among the many different options available can be a challenging task. Includes information on: Establishing an In Vitro Model System; Choosing an Endpoint to Measure; Characterizing Assay Responsiveness; Determining Dose and Duration of Exposure; Homogeneous Assays for Multiwell Formats and Automated Screening; Additional Factors to Consider When Choosing a Cell Viability Assay; Cell Viability Assays that Measure ATP Protocol; etc.. - [Read Cell Viability Information For Protocols and Applications]

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Rotors, Rotor Tubes, Relative Centrifugal Force, Rotor Comparisons. Article - [Read Centrifugation Information]

Category: Chromatography Protocols: Column Chromatography Protocols

Column Chromatography Protocol DOC, includes troubleshooting information. Equipment used included: Peristaltic pump, UV detector , Chart recorder, and Column. Prestegard Lab. - [Read Column Chromatography Protocol DOC]

Category: Microscopy Protocols: Confocal Microscopy Protocols

There are two major forms of laser scanning microscopy: confocal laser scanning microscopy (CLSM) and multiphoton laser scanning microscopy (MPLSM). Information on: X-t scans and X-Y scans; Confocal Laser Scanning Microscopy; Multiphoton Laser Scanning Microscopy; MPLSM requires no pin hole; Advantages of MPLSM over CLSM. - [Read Confocal Laser Scanning Microscopy]

Category: Microscopy Protocols: Confocal Microscopy Protocols

Basic information on confocal microscopy, includes: Specimen Preparation and Imaging; Objective Lens Parameters and Optical Section Thickness; The Objective Lens; Probes for Confocal Imaging; Autofluorescence; Collecting Images; Troubleshooting; Image Processing and Publication; - [Read Confocal Microscopy: Speciman Preparation and Imaging]