info Protocols

Category: PCR Protocols: Standard PCR Protocol

PCR and Multiplex Guide. Amazing Guide to primer amount, MgCl2 amount, annealing temperature, and more. With picture examples. Octavian Henegariu. - [Read PCR and Multiplex Guide]

Category: RNA Protocols: cDNA Libraries Library

"useful screen phage library non-radioactive, and not need too many clones. Up to 5 clones can be obtained usually within one week." Methods.info - [Read PCR based Lambda λDNA library screening method]

Category: PCR Protocols: PCR Buffer Protocols

Protocol for PCR buffers. - [Read PCR Buffers Protocol]

Category: PCR Protocols: Standard PCR Protocol

Protocol for PCR buffers. - [Read PCR Buffers Protocol]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

The requirement of an optimal PCR reaction is to amplify a specific locus without any unspecific by-products. Therefore, annealing needs to take place at a sufficiently high temperature to allow only the perfect DNA-DNA matches to occur in the reaction. P - [Read PCR Program Design]

Category: PCR Protocols: PCR Optimization

The requirement of an optimal PCR reaction is to amplify a specific locus without any unspecific by-products. Therefore, annealing needs to take place at a sufficiently high temperature to allow only the perfect DNA-DNA matches to occur in the reaction. P - [Read PCR Program Design]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR Technology. Informative info on PCR and Protocols. Connie Veilleux - [Read PCR Technology]

Category: PCR Protocols

PCR Technology. Informative info on PCR and Protocols. Connie Veilleux - [Read PCR Technology]

Category: Affiliates

Real Time PCR Information and protocols. - [Read Real Time PCR Info]

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Protocol for restriction analysis of DNA methylation. - [Read Restriction Analysis of DNA Methylation Protocol]

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Dr.A.Gratchev - [Read Rotor equilibration without counterbalance]

Category: Genetics and Genomics: Cancer Genetics Protocols

SAGE is a new method that has been invented at Johns Hopkins University in USA to give scientists an overview of a cell’s complete gene activity. It works by capturing RNAs, identifying them and counting them. By comparing different types of cells, the researchers hope to generate profiles that will help them understand healthy cells and what goes wrong during diseases. Includes: How SAGE works and Steps of SAGE. - [Read Serial Analysis Of Gene Expression (SAGE)]

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Protocol for a simplified Arabidopsis transformation. Found that the MS salts, hormone, etc. make no difference, that OD of bacteria doesn't make much of a difference, that vacuum doesn't even make much of a difference as long as you have a decent amount of surfactant present. Plant health is still a major factor - healthy fecund plants make a big difference! With this method you should be able to achieve transformation rates above 1%. - [Read Simple Arabidopsis Transformation Protocol]

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Single Nucleotide Primer Extension can be used for the analysis of methylation in a certain position. Treat DNA with bisulphite and then anneal a primer which ends immediately before the site of analysis. Dr. A. Gratchev Methods.info - [Read Singel Nucleotide Primer Extension (SNuPE)]

Category: Cytogenetics Protocols

Protocol for slide preparation for cytogenetics. Includes: Improved cell recovery by microcentrifugation; Slide storage; "Controlled" chromosome spreading; Dropping cells from a height does not improve spreading; GTG-banding. - [Read Slide Preparation for Cytogenetics Protocol]

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for small marker chromosome identification in metaphase and interphase using centromeric multiplex FISH (CM-FISH). This approach allows the identification of marker chromosomes and other aneuploides on one single cytogenetic slide, in less than two hours. - [Read Small Marker Chromosome Identification in Metaphase and Interphase using Centromeric Multiplex FISH]

Category: General Research Protocols and Methods: Laboratory Safety

Test Knowing How to Practice Safe Science. Yale University. A great online interactive test for lab safety. - [Read Test Knowing How to Practice Safe Science]

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for triple color FISH technique for mouse chromosome identification. - [Read Triple Color FISH Technique for Mouse Chromosome Identification Protocol]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Troubleshooting

Very Good Troubleshooting for PCR and multiplex PCR Guide. Octavian Henegariu. - [Read Troubleshooting for PCR and multiplex PCR]

Category: PCR Protocols: PCR Troubleshooting

Very Good Troubleshooting for PCR and multiplex PCR Guide. Octavian Henegariu. - [Read Troubleshooting for PCR and multiplex PCR]

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Streaking of Western Blots, Weak Bands or Weak Staining of Western Blots, Poor Transfer of Membranes, Dirty Blot, Multiple Bands on Western Blot Membrane and Film. Western Blot Info. - [Read Western Blot Troubleshooting]