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Adherent Cell Types Infection Protocol - http://www.med.umich.edu/vcore/protocols/RetroviralCellScreenInfection101705.pdf

Category: Cloning Protocols: Vector Protocols

Protocol for infection for adherent cell types. - [Read Adherent Cell Types Infection Protocol]

Bacterial Cell Maintenance Protocol - http://www.genome.ou.edu/protocol_book/protocol_partI.html#I.G

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

4 strains of E. coli are used in these studies: JM101 for M13 infection and isolation, XL1BMRF'for M13 or pUC-based DNA transformation, and ED8767 for cosmid DNA transformation. To maintain their respective F' episomes necessary for M13 viral infection, JM101 is streaked onto a M9 minimal media plate and XL1BMRF' is streaked onto an LB plate containing tetracycline. ED8767 is streaked onto an LB plate. These plates are incubated at 37degC overnight. For each strain, 3 ml. of appropriate liquid.. - [Read Bacterial Cell Maintenance Protocol]

Candida albicans Infection Protocol - http://www.natureprotocols.com/2006/06/29/candida_albicans_infection.php

Category: Fungi Protocols

Protocol for Candida albicans infection. - [Read Candida albicans Infection Protocol]

Cytokine Bioassays Protocols - http://www.ebioscience.com/ebioscience/appls/BAC.htm?

Category: Signalling Signal Transduction Protocols: Cytokine Protocols

Cytokine-Induced Proliferation of Indicator Cell Lines, TNF-Î±-Induced Killing of L929 Cell Line, IFN-Î³ Protection from Viral Infection of Cell Lines. eBiosciences. - [Read Cytokine Bioassays Protocols]

Cytokine Neutralization, In Vitro Using Antibodies - http://www.ebioscience.com/ebioscience/appls/NU.htm

Category: Signalling Signal Transduction Protocols: Cytokine Protocols

Antibody Neutralization of TNF-Î±-Induced Killing of L929 Cell Line, IFN-Î³-Protection from Viral Infection of L929 and A549 Cell Lines, TNF-Î±-Induced Killing of L929 Cell Line. eBiosciences. - [Read Cytokine Neutralization, In Vitro Using Antibodies]

Diseases of Xenopus tropicalis - http://tropicalis.berkeley.edu/home/husbandry/disease.html

Category: Xenopus Protocols

Information on the diseases of Xenopus tropicalis. Includes: PREMETAMORPHS: Bacterial Infection of Embryos; Protozoal Infestation of Tadpoles; POSTMETAMORPHS: Mycobacteriosis; Chytridiomycosis (Batrachochytrium dendrobatidis). - [Read Diseases of Xenopus tropicalis]

Infection Protocol for Adherent Cell Types - http://www.med.umich.edu/vcore/protocols/RetroviralCellScreenInfection101705.pdf

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

This protocol may be used for the infection of adherent cell lines. - [Read Infection Protocol for Adherent Cell Types]

Method for Assaying Gliotoxin Production in Aspergillus fumigatus Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./gliotoxin1.html~main

Category: Fungi Protocols: Aspergillus Protocols

Gliotoxin is a metabolite of Aspergillus fumigatus that exhibits immunosuppressive activity against certain cells of the immune system. Secretion of gliotoxin during infection has been suggested as being a factor in the pathogenesis of aspergillosis. Gliotoxin secretion can be assayed in a number of ways by thin layer chromatography (TLC) high performance liquid chromatography (HPLC) or bioassay using the effect of gliotoxin on human cells1. - [Read Method for Assaying Gliotoxin Production in Aspergillus fumigatus Protocol]

Plating Bacteriophage Lambda Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3961

Category: Viral Manipulation Protocols: Phage Protocols

Protocol describes a method for generating isolated plaques from a stock of bacteriophage lambda. Each plaque derives from infection of a single bacterium by a single bacteriophage particle. Because each plaque contains the progeny of a single virus particle, the bacteriophages derived from a single plaque are essentially genetically identical to one another. - [Read Plating Bacteriophage Lambda Protocol]

Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda}: Lytic Infection - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3771

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

This rapid method is used to screen bacteriophage {lambda}gt11 clones for the production of immunodetectable fusion proteins. After optimizng the conditions of infection and induction, the method can be used to produce preparative amounts of a fusion protein. - [Read Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda}: Lytic Infection]

Retroviral Infection of Primary Human Keratinocytes (PHKs) Protocol - http://www.methodbook.net/cellcult/infectn.html

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol for the retroviral infection of primary human keratinocytes (PHKs). - [Read Retroviral Infection of Primary Human Keratinocytes (PHKs) Protocol]

Transfection/Retroviral Infection pCL-Eco+Gene of Interest into 293T Cells Infecting HC11 Cells - http://www.bcm.edu/rosenlab/protocols/retroviral.pdf

Category: Cloning Protocols: Vector Protocols

Protocol for the transfection/Retroviral Infection pCL-Eco+Gene of interest into 293T cells infecting HC11 cells. Includes: Split 293T cells for transfection; Transfect 293T cells; Split HC11 cells for infection; Infect target (HC11) cells. - [Read Transfection/Retroviral Infection pCL-Eco+Gene of Interest into 293T Cells Infecting HC11 Cells]

Transient Transfection and Spin Infection Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1461

Category: Transfection Protocols: Transient Transfection Protocols

Protocol for transient transfection and spin infection. - [Read Transient Transfection and Spin Infection Protocol]

Use of “293-GPG” cells to Generate Amphotropic Retroviruses, plus Infection Protocol - http://www.flemingtonlab.com/Protocols/293amphMaintProdInfection.pdf

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Use of “293-GPG” cells to generate amphotropic retroviruses, plus infection protocol. - [Read Use of “293-GPG” cells to Generate Amphotropic Retroviruses, plus Infection Protocol]

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Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.