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Calcium Flux Using Indo-1 on the FACSVantage Protocol - http://www.cancer.umn.edu/images/research/cores/calcflux.jpg

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Protocol for calcium flux using Indo-1 on the FACSVantage. - [Read Calcium Flux Using Indo-1 on the FACSVantage Protocol]

Calcium Flux: Indo-1 Loading and Sample Staining Procedure for Simultaneous Measurement of Intra Ca2 - http://www.natureprotocols.com/2007/01/29/calcium_flux_indo1_loading_and.php

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Protocol for Calcium Flux: Indo-1 loading and sample staining procedure for simultaneous measurement of intracellular Ca2+. Includes: Preparation of INDO-1; Preparation of Ionomycin. - [Read Calcium Flux: Indo-1 Loading and Sample Staining Procedure for Simultaneous Measurement of Intra Ca2]

Indo-1 loading and sample staining procedure for simultaneous measurement of intracellular Ca2+ - http://www.natureprotocols.com/2007/01/29/calcium_flux_indo1_loading_and.php

Category: Signalling Signal Transduction Protocols: Calcium Measurement Protocols

Protocol by using the technique of Indo-1 loading and sample staining procedure for simultaneous measurement of intracellular Ca2+. Protocol includes: Preparation of Indo-1 and preparation of Ionomycin. - [Read Indo-1 loading and sample staining procedure for simultaneous measurement of intracellular Ca2+]

Measurement of Intracellular Ions by Flow Cytometry Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C4C9AC9935B31A1C2087E2276A3&objectid=6674EF32EE59A37C48B4A267D733CAE2

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

Describes flow cytometric protocols using the dyes Indo-1 AM, Fluo-3, and Fura Red AM to measure intracellular calcium concentration. Support protocols detail the use of calcium buffers to calibrate a flow cytometric calcium assay, and methods to facilitate dye loading; an alternate protocol describes the use of a spectrofluorimeter to measure intracellular calcium for those investigators without access to a flow cytometer. - [Read Measurement of Intracellular Ions by Flow Cytometry Protocol]

Simultaneous Measurement of Intracellular CA2+ and Cell Surface Antigen Expression Protocol - http://cyto.mednet.ucla.edu/Protocols/Calcium%20Staining.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

INDO-1 loading and sample staining protocol for simultaneous measurement of intracellular CA2+ and cell surface antigen expression. Includes: Preparation of INDO-1; Preparation of Ionomycin. - [Read Simultaneous Measurement of Intracellular CA2+ and Cell Surface Antigen Expression Protocol]

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Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

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