including Protocols

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols

This protocol is employed for the purification of a population of muscle-derived cells from skeletal muscle of C57Bl/6 animals. The resulting preparation is a mixture of many cell types including satellite cells (a.k.a., muscle progenitor cells) and hematopoietically active muscle-derived cells. - [Read Protocol for the Isolation of a Heterogenous Muscle-Derived Cell Population]

Category: Nucleic Acid Purification Protocols

Radiolabeled nucleic acids, including oligonucleotides, can be separated from unincorporated radiolabel by quantitative, differential precipitation with the cationic detergent cetylpyridinium bromide (CPB).  The nucleic acids are first precipitated from aqueous solution with CPB. - [Read Purification of Radiolabeled Oligonucleotides by Precipitation with Cetylpyridinium Bromide Protocol]

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

This protocol is not phenol-based, but does require the addition of chloroform. The Concert Plant Reagent is intended for the isolation of RNA from a wide variety of plant tissues including blue spruce needles, potato tuber etc. - [Read Purification of RNA from Plant Tissue Using the Concert Plant Reagent]

Category: General Research Protocols and Methods: Spectrophotometry Protocols

Spectrophotometric Measurement of Nucleic Acids' Concentration Tool. This bioinformatic program help calculate the concentration of nucleic acids according to optical density (including DNA, RNA, oligonucleotides). Zbio - [Read Spectrophotometric Measurement of Nucleic Acids' Concentration Tool]

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection protocol for the ecotropic/amphotropic producer line including titering and concentration of viral stocks. - [Read Transient Transfection Protocol: Viral Production]

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Fixation can mask epitopes. However, it is often possible to re-expose them using a gentle incubation with proteases, which removes obstructing structures and allows antibody access, as described here. Many proteases can be used for this procedure, including very crude preparations of proteases, such as pronase. However, using a better-characterized protease, such as trypsin, allows a more controlled reaction and better comparison between experiments. - [Read Unmasking Hidden Epitopes with Proteases Protocol]

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

A. tumefaciens is a soil-dwelling bacterium that transforms normal plant cells into tumor-forming cells by inserting a piece of bacterial DNA (the transfer, or "T," DNA) into the plant cell genome. The Ti plasmid also carries many of the transfer functions for mobilizing the T-DNA. This article provides a brief discussion of the principles of T-DNA transformation, including consideration of T-DNA vectors and their hosts. - [Read Vectors and Agrobacterium Hosts for Arabidopsis Transformation Protocol]

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Protocols for yeast media including: YNB1 1.1x agar, YNB2 1.1x liquid, YNB3 agar, YNB4 liquid, YNB5 Agarose, YP1 1.1x agar pH6, YP2 1.1x agar, YP3 1.1x liquid pH6, YP4 1.1x liquid, YPD1 agar pH6, YPD2 agar, YPD3 liquid pH6, YPD4 liquid. - [Read Yeast Media Protocols]