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Gradient Purification of AT2 Inactivated HIV-1 Protocol - http://ubik.microbiol.washington.edu/protocols/bl3/at2.htm

Protocol for gradient purification of AT2 inactivated HIV-1. - [Read Gradient Purification of AT2 Inactivated HIV-1 Protocol]

Isolation and handling of primary mouse embryonic fibroblasts (MEFs) - http://www.molgen.mpg.de/~rodent/MEF_protocol.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: MEF Protocols Mouse Embryonic Fibroblasts

Embryonic stem cells are usually grown on a layer of mitotically inactivated primary mouse embryonic fibroblasts to promote growth and prevent differentiation. Boris Greber - [Read Isolation and handling of primary mouse embryonic fibroblasts (MEFs)]

Protocol for Immunofluorescence - http://www.bio.unc.edu/faculty/salmon/lab/protocolsimmunofluorescence.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Protocol for Immunofluorescence - Salmon Lab. Making Boiled Donkey (or whatever) Serum (BDS) for Blocking. Making Heat-Inactivated Serum for Blocking. Mounting Media. - [Read Protocol for Immunofluorescence]

Articles (1-1 of 1)

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.