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Direct Sequencing Using MultiPlex PCR-Based Method - http://www.genome.ou.edu/MultiplexPCRbasedSeq.html

Category: DNA Protocols: DNA Sequencing Protocols

Direct sequencing from amplified bacterial and large insert cloned human or mouse genomic DNA via an improved MultiPlex PCR-based method. Includes: Preparing primers for MP-PCR; Amplification; PCR Product Clean-up; Sequencing. - [Read Direct Sequencing Using MultiPlex PCR-Based Method]

Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Anti - http://www.jhc.org/cgi/content/full/50/4/449

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Antibodies to Cleaved Caspase 3. Gown et al, 2002 - [Read Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Anti]

Methods for Synchronizing Cells at Specific Stages of the Cell Cycle - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662F47F15A7860600C31BDC6CCF455&objectid=6673FC95E03763BC6E766F163D5338A0

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Exponentially growing cells are asynchronous with respect to the cell cycle stage. Detection of cell cycle-related events is improved by enriching the culture for cells at the stage during which the particular event occurs. Methods for synchronizing cells are provided here, including those based on morphological features of the cell. - [Read Methods for Synchronizing Cells at Specific Stages of the Cell Cycle]

Molecular Cloning of PCR Products Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=3DC8DA9CD690052F2234D07559CD9BFE&objectid=6676A195E9797060CC491B4B58ECC2E1

Category: PCR Protocols: PCR Cloning Protocols

The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified fragments. This protocol describes the strategies for generating and manipulating suitable ends on the PCR fragments. - [Read Molecular Cloning of PCR Products Protocol]

Monster Green® Fluorescent Protein Assay - http://www.promega.com/catalog/country_select.asp?/tbs/tb320/tb320.html&ckt=2

Category: Reporter Gene Assays: GFP Assay Protocols

Green fluorescent protein is commonly used to monitor gene expression and protein trafficking within intact cells. The Monster Green® Fluorescent Protein is encoded by an improved synthetic version of the green fluorescent protein gene originally cloned from Montastrea cavernosa (Great Star Coral). - [Read Monster Green® Fluorescent Protein Assay]

Preparing Agrobacterium Cells That Simplifies the Arabidopsis Transformation Protocol - http://www.plantmethods.com/content/2/1/16

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Protocol for an improved method for preparing Agrobacterium cells that simplifies the Arabidopsis transformation protocol. - [Read Preparing Agrobacterium Cells That Simplifies the Arabidopsis Transformation Protocol]

Slide Preparation for Cytogenetics Protocol - http://info.med.yale.edu/genetics/ward/tavi/Cytom-slideprep.html

Category: Cytogenetics Protocols

Protocol for slide preparation for cytogenetics. Includes: Improved cell recovery by microcentrifugation; Slide storage; "Controlled" chromosome spreading; Dropping cells from a height does not improve spreading; GTG-banding. - [Read Slide Preparation for Cytogenetics Protocol]