Cryo-Immunogold Electron Microscopy Protocol- https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662E9BAD69DF0691FE8955F6EE7024&objectid=6673D13FDA2B0D70D4143DB7CECC80D9
Details of this protocol, Cryo-Immunogold Electron Microscopy, are located on a web site other than Protocols. Cryo-Immunogold Electron Microscopy Protocol - [Read Cryo-Immunogold Electron Microscopy Protocol]
Article describe the preparation of cells for correlative electron microscopy after live light microscopic observation of fluorescently labeled cytoskeletal proteins microinjected into the same cells. Since identification of cytoskeletal elements in electron microscopic preparations is an essential part of any correlative study, procedures for immunogold labeling of cytoskeletal components and for myosin S1 decoration of actin filaments are also described. - [Read Electron Microscopy of the Cytoskeleton of Cultured Cells]