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A semi-permanent mounting medium for immunofluorescence microscopy - http://www.hei.org/research/aemi/moviol.htm

Category: Molecular Imaging: Light Microscopy

A semi-permanent mounting medium for immunofluorescence microscopy. House Ear Institute - [Read A semi-permanent mounting medium for immunofluorescence microscopy]

Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4523

Category: C. elegans Protocols: C. elegans Staining Protocols

Extreme care should be used to identify and verify positive reactions, however, because cross-reactions are common. Counterstaining is essential for examining worms by immunofluorescence and is used to identify the exact cell in which an antigen appears. Methods for counterstaining include labeling all cells with a fluorescent dye that is specific for nucleic acids (e.g., DAPI or propidium iodide) and using GFP driven by tissue-specific promoters. - [Read Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol]

Basic Method for Direct Immunofluorescence Labeling - http://www.biotech.iastate.edu/facilities/CELLHYB/Labeling.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Basic Method for Direct Immunofluorescence Labeling. Iowa State University, Flow Cytometry Facility. - [Read Basic Method for Direct Immunofluorescence Labeling]

Blocking of unwanted non-specific staining in Immunofluorescence. - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/NonSpecificBlocking.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Blocking of unwanted non-specific staining in Immunofluorescence. Blocking of endogenous enzymes, Blocking of endogenous fluorochromes, Blocking endogenous biotin , Blocking of endogenous Fc blocking, Blocking of crossreactive antigens in the tissue. Cattoretti. Columbia University - [Read Blocking of unwanted non-specific staining in Immunofluorescence.]

Cell Fixation Using Methanol - http://www.lamondlab.com/pdf/CellFixMEOH.pdf

Category: Immunology: Immunocytochemistry Protocols

Methanol Fixation for Immunofluorescence. Lamond Lab, Univ. Dundee - [Read Cell Fixation Using Methanol]

Cell Staining for Immunofluorescence Microscopy - http://www.bdbiosciences.com/pharmingen/protocols/Immunofluorescence_Microscopy.shtml

Category: Molecular Imaging: Immunofluorescence Microscopy

Cell Staining for Immunofluorescence Microscopy. Includes protocols for fixing the cells, Coverslip Preparation for Adherent Cells, Coverslip Preparation for Non-Adherent Cells, Paraformaldehyde Fixation, and Methanol/Acetone Fixation. Blocking protocols include blocking with primary antibody, and incubation with secondary antibody. - [Read Cell Staining for Immunofluorescence Microscopy]

Confocal Microscopy Specimen Preparation Using Synthetic Fluorophores and Immunofluorescence - http://www.olympusconfocal.com/applications/protocols/index.html

Category: Microscopy Protocols: Confocal Microscopy Protocols

Includes staining protocols: Triple-Staining Adherent Cells with MitoTracker, Phalloidin,& Nuclear Dyes; Staining Adherent Cells with Cytokeratin Primary Antibodies & Synthetic Fluorophores; Staining Adherent Cells with Intermediate Filament Primary Antibodies & Synthetic Fluorophores; Staining Cells & Tissue Cryosections with Tubulin Primary Antibodies, Phallotoxins,& Synthetic Fluorophores; Triple-Staining Tissue Cryosections with Wheat Germ Agglutinin, Phalloidin,& Nuclear Dyes; etc. - [Read Confocal Microscopy Specimen Preparation Using Synthetic Fluorophores and Immunofluorescence]

Detection of RNAi-Induced Protein Knockdown in Mammalian Cells by Western Blotting Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4345

Category: RNAi Technology Protocols: RNAi Mammalian Cells Protocols

Protocol describes the use of western blotting to analyze the RNAi-induced depletion of target protein from mammalian cells. Immunofluorescence can be used as an alternative. - [Read Detection of RNAi-Induced Protein Knockdown in Mammalian Cells by Western Blotting Protocol]

Detection of RNAi-Induced Protein Knockdown in Mammalian Cells by Western Blotting Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4345

Category: Western Blot Protocols

Double immunofluorescence staining for BCL-6 - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/DoubleIFstain.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Double immunofluorescence staining for BCL-6. unfixed or acetone-fixed specimens. dewaxed, antigen retrieved slides - [Read Double immunofluorescence staining for BCL-6]

Double Immunofluorescence Staining for BCL-6 Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/DoubleIFstain.html

Category: Immunology: Immunofluorescence Protocols: Immunofluorescence Staining Protocols

Protocol for double immunofluorescence staining for BCL-6. Any type of tissue is suitable for this technique, as long as the antigenicity for your antigen(s) is preserved. Includes: unfixed or acetone-fixed specimens; dewaxed, antigen retrieved slides. - [Read Double Immunofluorescence Staining for BCL-6 Protocol]

Flow Cytometry Analysis Protocol - http://www.cbrinstitute.org/labs/springer/protocols/qing_facs.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules. Includes: Immunofluorescence Staining and Flow Cytometry Analysis. - [Read Flow Cytometry Analysis Protocol]

Flow Cytometry Analysis Protocol - http://www.cbrinstitute.org/labs/springer/protocols/qing_facs.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules. Includes: Immunofluorescence Staining; Flow Cytometry Analysis. - [Read Flow Cytometry Analysis Protocol]

Fluorescent Staining of Cell Surface Antigens on Viable Cells Using Direct Immunofluorescence - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4439

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Protocol provides a method for analysis of cell surface antigens using direct immunofluorescence, i.e., when the antibody being used is directly labeled to a fluorescent dye. After staining, the cells can be analyzed immediately by flow cytometry or fixed and stored for later analysis. - [Read Fluorescent Staining of Cell Surface Antigens on Viable Cells Using Direct Immunofluorescence]

Fluorescent Staining of Cells - http://ylwang.umassmed.edu/protocol/cfs/stain.htm

Category: Molecular Imaging: Immunofluorescence Microscopy

Fluorescent Staining of Cells protocol. Yu-li Wang Lab. Fluorescent Phalloidin Staining, Immunofluorescence Staining and procedures for staining. - [Read Fluorescent Staining of Cells]

Gene Expression and Protein Localization in Arabidopsis Protocols - http://www.arabidopsis.org/cshl-course/7-gene_expression.html

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocols for gene expression and protein localization in Arabidopsis. Includes: Detection of the native protein; Detection of a recombinant version; Immunofluorescence detection in Arabidopsis protoplasts; Isolation of Arabidopsis seedling protoplasts; Subcellular localization of GUS-fusion proteins in Arabidopsis seedlings; Localization of Arabidopsis proteins with GUS in situ enzyme assay. - [Read Gene Expression and Protein Localization in Arabidopsis Protocols]

General Immunofluorescence Protocol - http://www.bcm.edu/rosenlab/protocols/generalIF.pdf

Category: Molecular Imaging: Immunofluorescence Microscopy

General Immunofluorescence Protocol. Rosen Lab. Deparaffinize and rehydrate sections, Antigen retrieval methods, Proteinase K Antigen Retrieval, Urea Antigen Retrieval, Blocking buffers, - [Read General Immunofluorescence Protocol]

High-Throughput Immunostaining Protocol - http://openwetware.org/wiki/Silver:_High-throughput_immunostaining

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for high-throughput immunostaining. Includes: [edit] Detection of 14-3-3 in U2OS and HeLa cells by immunofluorescence. - [Read High-Throughput Immunostaining Protocol]

Immunofluorescence - http://www.kcl.ac.uk/depsta/biomedical/randall/methods/im-fluo.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Immunofluorescence. Dr. Walter Steffen. Includes fixation and staining protocols. - [Read Immunofluorescence]

Immunofluorescence / confocal microscopy - http://depts.washington.edu/keck/susan.htm

Category: Molecular Imaging: Immunofluorescence Microscopy

B or T cells in suspension, adherent cells on chambered coverglass or chamberslides, cryostat sections of unfixed, OCT embedded tissue. Susan Anderson. - [Read Immunofluorescence / confocal microscopy]

Immunofluorescence Detection of RNAi-Induced Protein Knockdown in Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4344

Category: Immunology: Immunofluorescence Protocols

This protocol describes the use of a specific antibody that recognizes the targeted gene product to detect RNAi-induced gene knockdown in mammalian cells. Western blot technology can be used as an alternative (see Detection of RNAi-Induced Protein Knockdown in Mammalian Cells by Western Blotting). - [Read Immunofluorescence Detection of RNAi-Induced Protein Knockdown in Mammalian Cells Protocol]

Immunofluorescence Detection of RNAi-Induced Protein Knockdown in Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4344

Category: RNAi Technology Protocols: RNAi Mammalian Cells Protocols

Protocol describes the use of a specific antibody that recognizes the targeted gene product to detect RNAi-induced gene knockdown in mammalian cells. Western blot technology can be used as an alternative. - [Read Immunofluorescence Detection of RNAi-Induced Protein Knockdown in Mammalian Cells Protocol]

Immunofluorescence Labeling of Cells Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Antibody_Explorer/Procedures/Immunofluorescence.html

Category: Immunology: Immunofluorescence Protocols

Protocol for immunofluorescence labeling of cells. Includes: Cell Preparation; Fixation; Application of Primary Antibody; Application of Secondary Antibody and Evaulation. - [Read Immunofluorescence Labeling of Cells Protocol]

Immunofluorescence of C. elegans Embryos Protocol - http://www-biology.ucsd.edu/labs/aroian/protocols/immunofluorescence.htm

Category: C. elegans Protocols

Protocol for immunofluorescence of C. elegans embryos. - [Read Immunofluorescence of C. elegans Embryos Protocol]

Immunofluorescence Protocol - http://www.abgent.com/doc/if1

Category: Molecular Imaging: Immunofluorescence Microscopy

Immunofluorescence Protocol. ABGent. Includes tips and considerations for improving protocol. - [Read Immunofluorescence Protocol]

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