immunocytochemistry Protocols

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Combined DNA In Situ Hybridization and Immunocytochemistry Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_127-134.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

Protocol for combined DNA in situ hybridization and immunocytochemistry for the simultaneous detection of nucleic acid sequences, proteins, and incorporated BrdU in cell preparations. Includes: Cell preparations and BrdU labeling; Detection of antigen by immunocytochemistry (ICC); Visualization of ICC antigen; -Gal-BCIG reaction (for producing a blue precipitate visible under brightfield microscopy); Cell processing for in situ hybridization; In situ hybridization (ISH); etc... - [Read Combined DNA In Situ Hybridization and Immunocytochemistry Protocol]

Enzymatic Protocol for Immunocytochemistry - http://www.rndsystems.com/ihc_detail_objectname_EnzymaticProtocol20.aspx

Category: Immunology: Immunocytochemistry Protocols

Staining Tissue, Staining Cells Smeared on Slides. Positive staining controls, Negative staining controls, Immunoenzymatic Technique and FAQ. R & D Systems. - [Read Enzymatic Protocol for Immunocytochemistry]

Flow Cytometric Analysis of Mitochondrial Transmembrane Potential ({Delta}{Psi}m) - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4465

Category: Cell Organelle Protocols: Mitochondria Protocols

Protocol describes a method for evaluation of mitochondrial function using the fluorochrome CMXRos. CMXRos is sequestered by actively respiring mitochondria, but washed out when the mitochondrial membrane potential is lost. This analysis can be combined with the TUNEL technique or immunocytochemistry. - [Read Flow Cytometric Analysis of Mitochondrial Transmembrane Potential ({Delta}{Psi}m)]

Fluorescence Protocol Immunocytochemistry - http://www.rndsystems.com/ihc_detail_objectname_FluorescenceProtocol20.aspx

Category: Immunology: Immunocytochemistry Protocols

Staining Cells in Suspension, Staining Cells Smeared on Slides. Immunofluorescent Technique. R & D Systems. - [Read Fluorescence Protocol Immunocytochemistry]

Immunocytochemistry - http://cancer.ucsd.edu/howelllab/Immunocyto.html

Category: Immunology: Immunocytochemistry Protocols

Method for plating cells, fixing cells, antibody incubation and mounting of slides. PHEM buffer, Antifade recipes. Howell Lab. - [Read Immunocytochemistry]

Immunocytochemistry in Free-Floating Sections Protocol - http://www.fisher.co.uk/techzone/life/table-pdfs/protocol/immunocytochemistry_netwells_protocol.pdf

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

There are several strategies to visualize the antibody. For transmitted light microscopy, color development substrates for enzymes are often used. The antibody can be directly labeled with the enzyme. However, such a covalent link between an antibody and an enzyme might result in a loss of both enzyme and antibody activity. For these reasons several multistep staining procedures have been developed, where intermediate link antibodies are used. In this protocol use the Vectastain ABC-kit. - [Read Immunocytochemistry in Free-Floating Sections Protocol]

Immunocytochemistry with Adherent Cells - http://www.upstate.com/misc/protocols.q.prot.e.iccpro/Immunocytochemistry+with+Adherent+Cells

Category: Immunology: Immunocytochemistry Protocols

Fixatives commonly used for Immunocytochemistry for adherent cells. Upstate. - [Read Immunocytochemistry with Adherent Cells]

Microscopic Analysis of Mitochondrial Transmembrane Potential Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4462

Category: Cell Organelle Protocols: Mitochondria Protocols

Protocol describes a method for the evaluation of mitochondrial function using the fluorochrome CMXRos. CMXRos is sequestered by actively respiring mitochondria, but washed out when the mitochondrial membrane potential is lost. This analysis can be combined with the TUNEL technique or immunocytochemistry. - [Read Microscopic Analysis of Mitochondrial Transmembrane Potential Protocol]

Preparation of fixed Sea Urchin embryos for immunocytochemistry and AP staining - http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Urchin/Urchin_fix.html

Category: Developmental Biology: Sea Urchin Developmental Biology

Preparation of fixed Sea Urchin embryos for immunocytochemistry and AP staining. Cebra-Thomas. Swathmore. - [Read Preparation of fixed Sea Urchin embryos for immunocytochemistry and AP staining]