immunoaffinity Protocols

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Antigen Design and Sera Purification - http://www.sigmaaldrich.com/Brands/Sigma_Genosys/Custom_Antisera/Key_Resources/Antigen_Design.html

Category: Immunology: Antigen

Antigen Design and Sera Purification. Custom antisera. Sigma Aldrich. Peptide Selection and Design, Coupling Strategy # Selecting the Protein Carrier, Multiple Antigenic Peptides (MAPs), Choice of Host, Adjuvant, Immunization, & Sera Collection, Antisera Purification, Ammonium Sulfate Precipitation, Protein A/G, Immunoaffinity Purification. - [Read Antigen Design and Sera Purification]

Chromatin Immunoprecipitation and Microarray-Based Analysis of Protein Location Protocol - http://www.nature.com/nprot/journal/v1/n2/abs/nprot.2006.98.html

Category: Immunology: Immunoprecipitation Protocols: Chromatin Immunoprecipitation Protocols

Genome-wide location analysis, also known as ChIP-Chip, combines chromatin immunoprecipitation and DNA microarray analysis to identify protein-DNA interactions that occur in living cells. Protein-DNA interactions are captured in vivo by chemical crosslinking. Cell lysis, DNA fragmentation and immunoaffinity purification of the desired protein will co-purify DNA fragments that are associated with that protein. - [Read Chromatin Immunoprecipitation and Microarray-Based Analysis of Protein Location Protocol]

Enzyme Immunoaffinity Chromatographyâ€”A Rapid Semi-quantitative Method - http://www.rsc.org/delivery/_ArticleLinking/DisplayArticleForFree.cfm?doi=a607578i

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Enzyme Immunoaffinity Chromatographyâ€”A Rapid Semi-quantitative Method. - [Read Enzyme Immunoaffinity Chromatographyâ€”A Rapid Semi-quantitative Method]

Immunoaffinity Purification Gibberellins - PDF - http://www.plantphysiol.org/cgi/reprint/90/2/445.pdf

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Immunoaffinity Purification Gibberellins. PDF. - [Read Immunoaffinity Purification Gibberellins - PDF]

Immunoaffinity Purification: Coupling Antibodies to Protein A or G Bead Columns Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4303

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Protocol describes how antibodies are bound to protein A or protein G beads and are then crosslinked to the matrix via a bifunctional coupling reagent called dimethyl pimelimidate (DMP). - [Read Immunoaffinity Purification: Coupling Antibodies to Protein A or G Bead Columns Protocol]

Immunoaffinity Purification: Eluting Antigens from Immunoaffinity Columns Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4276

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

This elution procedure relies on disrupting the bonds between the antibody and the antigen. - [Read Immunoaffinity Purification: Eluting Antigens from Immunoaffinity Columns Protocol]

Purification of Antibodies on an Antigen Column Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4284

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Immunoaffinity purification of antibodies is used to purify antigen-specific antibodies from a preparation of polyclonal antibodies. Such purification is commonly needed in the production of antipeptide antibodies, where it is used to concentrate the desired antibodies and separate them from those raised against carrier proteins. It is also used for the more general purpose of removing unwanted, nonspecific binding activity from polyclonal antibody preparations. - [Read Purification of Antibodies on an Antigen Column Protocol]

Purification of the Adipocyte Insulin Receptor by Immunoaffinity Chromatography - http://www.jbc.org/cgi/reprint/255/4/1732.pdf

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Purification of the Adipocyte Insulin Receptor by Immunoaffinity Chromatography. - [Read Purification of the Adipocyte Insulin Receptor by Immunoaffinity Chromatography]