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Protein Precipitation - http://www.aai.org/committees/education/Curriculum/protein.htm

Category: Protein Protocols: Protein Precipitation Procols

Protein Precipitation. Developed by: Eric Burtson. American Association of Immunologists 1995. In this lab you will separate a solution of proteins using protein precipitation. Since you will be testing the same protein mix that you used in the Gel Filtration lesson, you will pass the protein through a gel filtration column to identify which protein(s) precipitates. - [Read Protein Precipitation]

Screening DNA Pools for T-DNA Insertions in Arabidopsis Genes Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4622

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

A powerful way to identify a mutation in the gene of interest and to test mutant plants for phenotypes that are predicted to result from loss of function of that gene is by PCR screening. Pools of insertion lines are screened using one primer corresponding to the gene of interest and one primer corresponding to the end of the insertion element. The synthesis of a product indicates the presence of an insertion in the gene of interest. - [Read Screening DNA Pools for T-DNA Insertions in Arabidopsis Genes Protocol]

Single Nucleotide Polymorphisms as Tools in Human Genetics - http://www.nslij-genetics.org/ld/gray00.pdf

Category: Genetics and Genomics: Genotyping Protocols

Describe the current efforts to identify and characterize the large number of SNP's required and discuss the practicalities of association studies for the identification of genes involved in complex traits. - [Read Single Nucleotide Polymorphisms as Tools in Human Genetics]

Subcloning of Yeast Artificial Chromosomes into Phage Lambda Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast9.html

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol for the subcloning of Yeast artificial chromosome into phage lambda. To subclone the large insert fragment of human DNA contained within a YAC into a bacteriophage lambda vector. The subclones are 15 to 23 kb in size, and can be used to identify new polymorphic markers from a known region of the genome, to map a specific locus, and/or to screen other libraries. - [Read Subcloning of Yeast Artificial Chromosomes into Phage Lambda Protocol]

Transfer of Bacteriophage DNA from Plaques to Filters Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3982

Category: Viral Manipulation Protocols: Phage Protocols

Protocol is used to identify and isolate recombinants containing DNA sequences of interest. - [Read Transfer of Bacteriophage DNA from Plaques to Filters Protocol]

Transgenic Outline: Mice and Rats - http://www.med.umich.edu/tamc/tgoutline.html

Category: Transgenic Mouse Protocols

Clone the Transgene, transgene Screening, Transgene Expression, DNA microinjection, Identify Transgenic Founders, Transgenic Analysis. Transgenic Animal Web. - [Read Transgenic Outline: Mice and Rats]

Articles (1-2 of 2)

Lambda Phage Protocol Large DNA Preparation

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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