hypersensitive Protocols

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Dnase-chip Protocol - http://www.natureprotocols.com/2006/07/03/dnasechip_a_high_resolution_me.php

Category: Microarray Protocols: Microarray Chip Protocols

Dnase-chip: A High Resolution Method to Identify DnaseI Hypersensitive Sites using Tiled Microarrays. This method can be applied globally or in a targeted fashion to any tissue from any species with a sequenced genome. - [Read Dnase-chip Protocol]

Mapping Dnase-I-hypersensitive Sites Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3949

Category: DNA Protein Interactions Protocols: Dnase Footprinting Assay Protocols

Protocol describes how isolated nuclei are incubated with varying amounts of Dnase I. Genomic DNA is then isolated from the nuclei and digested with a restriction enzyme, analyzed by gel electrophoresis, and probed by Southern hybridization. - [Read Mapping Dnase-I-hypersensitive Sites Protocol]