hybridoma Protocols

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Freezing and Thawing of Mammalian Cell Lines - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/CellFreeze.html

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing and Thawing of Mammalian Cell Lines. For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture. Kitto Lab Texas. - [Read Freezing and Thawing of Mammalian Cell Lines]

Freezing and Thawing of Mammalian Cell Lines Protocol - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/CellFreeze.html

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture. - [Read Freezing and Thawing of Mammalian Cell Lines Protocol]

Luciferase Assays in OT-1 Hybridoma Cells Protocol - http://www.natureprotocols.com/2007/01/29/luciferase_assays_in_ot1_hybri.php

Category: Reporter Gene Assays: Luciferase Assay Protocols

Protocol describing luciferase assays in OT-1 hybridoma cells. - [Read Luciferase Assays in OT-1 Hybridoma Cells Protocol]

Monoclonal Antibody Production - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/MAb.html

Category: Antibody Protocols: Antibody Production Protocols

Monoclonal Antibody Production. Includes: Mouse Immunization; Bleeding Mice; Hybridoma Fusion; Assaying for Positive Clones; Expanding Positive Clones. - [Read Monoclonal Antibody Production]

Presenting Exogenous Antigen to T Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6631B80A848F54654FC872A48DB060&objectid=6675843EDB95D0444CF7AA285D1714C8

Category: Immunology: Mononuclear Cell Isolation Protocols: T-Cell Isolation Protocols

Protocols utilize T hybridomas to detect expression of peptide-MHC complexes, since these cells provide the most convenient, consistent, and flexible T cell readout systems for these purposes. If desired, antigen-specific T cell clones can be used in lieu of T hybridoma cells, but T cell clones often give poorer responses than T hybridomas to fixed APCs due to fixation-induced loss of costimulator function. - [Read Presenting Exogenous Antigen to T Cells Protocol]

Steps in Hybridoma Production Protocol - http://herpesvirus.tripod.com/research/HYBRIDOM.htm

Category: Antibody Protocols: Antibody Production Protocols

Protocol for steps in hybridoma production. Includes: SIMPLE METHOD: BY FROM GIBCO the HAT and HT concentrate; INSTRUMENTS FOR SPLENECTOMY OF ONE MICE; PREPARATION FOR CELL FUSION; CELL FUSION; SCREENING THE CLONES; RECLONING; ASCITIC FLUID PRODUCTION; PREPARATION OF PERITONEAL MACROPHAGES. - [Read Steps in Hybridoma Production Protocol]

Storage of Tissue Culture Supernatants Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4280

Category: Antibody Protocols: Antibody Handling Protocols

This protocol describes the methods of storage for hybridoma tissue-culture supernatants. - [Read Storage of Tissue Culture Supernatants Protocol]