User Panel

My Panel

Bookmark Science Articles

hybridization Protocols

Search results for: hybridization

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Identification of Single Bacterial Cells using DIG-Labeled Oligonucleotides Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_139-142.pdf

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for the identification of single bacterial cells using DIG-labeled oligonucleotides. Includes: Organisms and growth conditions; Cell fixation and preparation of cell smears; DIG labeling of oligonucleotides with DIG-ddUTP; In situ hybridization using digoxigenin-labeled oligonucleotides; Detection of DIG-labeled oligonucleotides with fluorescently labeled anti-DIG Fab fragments; Detection of DIG-labeled oligonucleotide. - [Read Identification of Single Bacterial Cells using DIG-Labeled Oligonucleotides Protocol]

Identification of Single Bacterial Cells Using DIG-Labeled Oligonucleotides Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_139-142.pdf

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Protocol for identification of single bacterial cells using DIG-labeled oligonucleotides. Includes: Organisms and growth conditions; Cell fixation and preparation of cell smears; DIG labeling of oligonucleotides with DIG-ddUTP; In situ hybridization using digoxigenin-labeled oligonucleotides. - [Read Identification of Single Bacterial Cells Using DIG-Labeled Oligonucleotides Protocol]

Immunoprecipitation of Homer 1a, Injection of Virions and In Situ Hybridization in the Spinal Cord - http://www.natureprotocols.com/2006/06/30/immunoprecipitation_ip_of_home.php

Category: Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols

Protocol for the immunoprecipitation (IP) of Homer 1a, injection of virions and in situ hybridization in the spinal cord. Includes: Immunoprecipitation (IP) of Homer1a from spinal cord; Injection of virions in the parenchyma of the spinal dorsal horn in vivo; Generation of cRNA probes; Analysis of DIG-dUTP incorporation; Tissue hybridization. - [Read Immunoprecipitation of Homer 1a, Injection of Virions and In Situ Hybridization in the Spinal Cord]

In situ Hybridization - http://osiris.rutgers.edu/~smm/in_situ_hybridization.htm

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

Describes the basic principles of in situ hybridization and advantages and disadvantages of different methodologies that can be used. Includes: Probe Selection; Probe Generation; Probe Labels; Fixation of Tissue; Hybridization and Washing; Control Procedures. - [Read In situ Hybridization]

In Situ Hybridization for Epstein Barr Virus-Encoded RNA (EBER) Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/EBER-ISH4doublestain.html

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for in situ hybridization for Epstein Barr virus-encoded RNA (EBER). Includes: Glassware decontamination; Double staining for EBER and a second antibody . - [Read In Situ Hybridization for Epstein Barr Virus-Encoded RNA (EBER) Protocol]

In Situ Hybridization Protocol - http://www.k-state.edu/hermanlab/protocols/in_situ_hybridization.htm

Category: C. elegans Protocols

Protocol for in situ hybridization. - [Read In Situ Hybridization Protocol]

In Situ Hybridization to Human Metaphase Chromosomes using DIG-, Biotin- or Fluorochrome-Labeled DNA - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_78-94.pdf

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for In situ hybridization to human metaphase chromosomes using DIG-, biotin-, or fluorochrome-labeled DNA probes and detection with fluorochrome conjugates. Includes: Pretreatment of metaphase spreads on slides; Denaturation and hybridization; Single color fluorescent detection with immunological amplification; Multicolor fluorescence in situ hybridization (Multicolor FISH); Results obtained with human metaphase chromosome spreads. - [Read In Situ Hybridization to Human Metaphase Chromosomes using DIG-, Biotin- or Fluorochrome-Labeled DNA]

In Situ Hybridization to Human Metaphase Chromosomes Using DIG-, Biotin-, or Fluorochrome-Labeled - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_78-94.pdf

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Protocol for in situ hybridization to human metaphase chromosomes using DIG-, biotin-, or fluorochrome-labeled DNA probes and detection with fluorochrome conjugates. - [Read In Situ Hybridization to Human Metaphase Chromosomes Using DIG-, Biotin-, or Fluorochrome-Labeled]

In Situ Hybridization to Tissue Sections Protocol - http://cmgm.stanford.edu/~vesicle/protocols/in_situ.html

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for in situ hydbridization to tissue sections. Includes: Paraffin Sections; Frozen Sections; Probe preparation; Hybridization and washes; Autoradiography. - [Read In Situ Hybridization to Tissue Sections Protocol]

In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells - http://www.bio.com/protocolstools/protocol.jhtml?id=p1971

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

With this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are used. Includes information on: Permeabilization of Cells; In vitro Transcription Reaction (Run-off); Isolation of RNA; Preparation of Slot Blot Membrane for Hybridization; Hybridization of Nitrocellulose Membrane; TCA Precipitation to Determine Incorporation of [32P] GTP into Nucleic Acid - [Read In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells]

Isolation of DNA from Mammalian Cells Grown in 96-well Microtiter Plates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3695

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

Protocol for isolation of DNA from mammalian cells grown in 96-well microtiter plates. Each well yields sufficient genomic DNA for several standard PCRs or for analysis in a single lane of a Southern hybridization. - [Read Isolation of DNA from Mammalian Cells Grown in 96-well Microtiter Plates Protocol]

Labeled Probe Hybridization Procedure - http://pga.tigr.org/sop/M005_1a.pdf

Category: DNA Microarray Protocols

This protocol describes the hybridization of a Cy labeled cDNA probe (mix of Cy3 and Cy5) onto coated slide spotted with PCR amplified cDNA. TIGR Microarray Protocols. - [Read Labeled Probe Hybridization Procedure]

Mapping Dnase-I-hypersensitive Sites Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3949

Category: DNA Protein Interactions Protocols: Dnase Footprinting Assay Protocols

Protocol describes how isolated nuclei are incubated with varying amounts of Dnase I. Genomic DNA is then isolated from the nuclei and digested with a restriction enzyme, analyzed by gel electrophoresis, and probed by Southern hybridization. - [Read Mapping Dnase-I-hypersensitive Sites Protocol]

Mapping RNA with Nuclease S1 Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4053

Category: Nucleic Acid Purification Protocols

Preparations of RNA containing an mRNA of interest are hybridized to a complementary single-stranded DNA probe. At the end of the reaction nuclease S1 is used to degrade unhybridized regions of the probe, and the surviving DNA-RNA hybrids are then separated by gel electrophoresis and visualized by autoradiography or Southern hybridization. Method used to quantitate RNAs, to map the positions of introns, and to identify the locations of 5' and 3' ends of mRNAs on cloned DNA templates. - [Read Mapping RNA with Nuclease S1 Protocol]

Method: Transfer of DNA from Bacterial Colonies to Nylon Membrane or Nitrocellulose - http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid04.html

Category: RNA Protocols: cDNA Libraries Library

Purpose: To transfer DNA from bacterial colonies to nylon (or nitrocellulose) membrane so that a library can be screened by hybridization. Jim Howe Donis Keller Lab - [Read Method: Transfer of DNA from Bacterial Colonies to Nylon Membrane or Nitrocellulose]

Microarray Protocol for Affymetrix In Situ Synthesized Oligo Arrays - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000174.pdf?pid=PP00000174

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol details the preparation of biotin-labeled target samples and hybridization of these samples to an Affymetrix in situ synthesized oligonucleotide GeneChip array. The procedure requires a minimum of 5 µg of purified total RNA as starting material. - [Read Microarray Protocol for Affymetrix In Situ Synthesized Oligo Arrays]

Microarray Protocol for Agilent Inkjet-Deposited Presynthesized - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000184.pdf?pid=PP00000184

Category: Microarray Protocols: Amino-allyl Microarray Methods

Protocol details the preparation of fluorescently labeled target samples (aminoallyl method) and hybridization of these samples to a microarray of Agilent inkjet-deposited presynthesized oligonucleotides. The procedure requires a minimum of 3 µg of purified total RNA as starting material. - [Read Microarray Protocol for Agilent Inkjet-Deposited Presynthesized]

Microarray Protocol for Agilent Inkjet-Deposited Presynthesized Oligo Array - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000179.pdf?pid=PP00000179

Category: Microarray Protocols: Microarray DNA Labeling Protocols

Protocol details the preparation of fluorescently labeled target samples and hybridization of these samples to a microarray of Agilent inkjet-deposited presynthesized oligonucleotides. The procedure requires a minimum of 3 µg of purified total RNA as starting material. - [Read Microarray Protocol for Agilent Inkjet-Deposited Presynthesized Oligo Array]

Microarray Protocol for Agilent Inkjet-Deposited Presynthesized Oligo Arrays - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000179.pdf?pid=PP00000179

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol details the preparation of fluorescently labeled target samples and hybridization of these samples to a microarray of Agilent inkjet-deposited presynthesized oligonucleotides. The procedure requires a minimum of 3 µg of purified total RNA as starting material. Includes: cDNA Synthesis; Fluorescent cRNA Synthesis; cRNA Precipitation and Cleanup; cRNA Quantification; Hybridization; Washing. - [Read Microarray Protocol for Agilent Inkjet-Deposited Presynthesized Oligo Arrays]

Microarray Protocols - http://www.etox.ucr.edu/gill/Microarray_Protocols.html

Category: DNA Microarray Protocols

Heather R. Sanders. Hybridization, Probe Creation and RNA preparation for Microarray Protocols. - [Read Microarray Protocols]

Microarray Protocols - http://www.erasmusmc.nl/interne_oncologie/res/endo/Protocol%20for%20RNA%20amplification%2C%20labelling%20and%20hybridization%20to%20%20microarray.pdf

Category: Microarray Protocols: Microarray Analysis Protocols

Protocols for microarrays. Includes: RNA amplification by in vitro transcription; cDNA labeling; Hybridization of cDNA microarrays. - [Read Microarray Protocols]

Microarrays a Complete Manual PDF - http://research.nhgri.nih.gov/microarray/Protocols.pdf

Category: DNA Microarray Protocols

Microarray fabrication, coating, slide blocking, printing microarrays, RNA extraction, Sample Labeling for analysis, hybridization, complete protocol. NHGRI - [Read Microarrays a Complete Manual PDF]

Microdissection Overview - http://cgap-mf.nih.gov/Protocols/Microdissection/Overview.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Human tissues are comprised of multiple interacting cell populations in a complex three dimensional arrangement with each cellular phenotype determined by a unique profile of mRNA and protein expression. Before microdissection techniques were developed, the only analysis tools for phenotypic studies were primarily immunohistochemistry and in-situ hybridization. While useful, these tools are limited to single gene analysis and, in general, do not allow qualitative studies. - [Read Microdissection Overview]

Molecular Analysis and Results - DNA Comparative Genomic Hybridization (CGH) - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/DNA/CGH.html

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

Based on the original protocol describing CGH by Kallioniemi et al. - [Read Molecular Analysis and Results - DNA Comparative Genomic Hybridization (CGH)]

mRNA In Situ Hybridization with Nick-Translated Probes Protocol - http://spectorlab.cshl.edu/in_situ_hybridization.html

Category: Cytogenetics Protocols: RNA In Situ Hybridization Protocols

Protocol for mRNA in situ hybridization with nick-translated probes. - [Read mRNA In Situ Hybridization with Nick-Translated Probes Protocol]

Articles (1-2 of 2)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

[1-2]

Items 51-75 out of 125 displayed.

First Previous 1 2 [3] 4 5 Next Last

Total records: 125