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Detection of HPV 11 DNA in Paraffin-Embedded Laryngeal Tissue with a DIG-Labeled DNA Probe Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_143-148.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for detection of HPV 11 DNA in paraffin-embedded laryngeal tissue with a DIG-labeled DNA probe. Includes: Probe preparation; Pretreatment of slides; Preparation of tissue sections; Hybridization; Washes; Detection of hybrids. - [Read Detection of HPV 11 DNA in Paraffin-Embedded Laryngeal Tissue with a DIG-Labeled DNA Probe Protocol]

DIG Labeling of cDNA Probes Protocol - http://home.cc.umanitoba.ca/~frist/Protocols/arrays/DIG_probes.html

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for DIG labeling of cDNA probes. Includes: Incorporation DIG during PCR; Estimating the yield of DIG labeled probes; DNA Dot Blotting; Prehybridization and Hybridization. - [Read DIG Labeling of cDNA Probes Protocol]

DIG Labeling of cDNA Probes Protocol - http://home.cc.umanitoba.ca/~frist/Protocols/arrays/DIG_probes.html

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Direct Selection of cDNAs with Large Genomic DNA Clones Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4077

Category: Genetics and Genomics: Genotyping Protocols: Mutation Detection Protocols

The goal of this method is to identify transcriptionally active genes in cloned segments of genomic DNA. The protocol uses hybridization and affinity purification to recover biotin-labeled cDNAs that bind to a 500-kb segment of human DNA cloned in a BAC vector. However, the method can be easily adapted to other clones of genomic DNAs cloned in high-capacity vectors. - [Read Direct Selection of cDNAs with Large Genomic DNA Clones Protocol]

DNA In Situ Hybridization with an Alkaline Phosphatase-Based Fluorescent Detection System - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_124-126.pdf

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol describes here a high sensitivity indirect detection procedure for DIG-labeled hybridization probes. The procedure uses the components of the HNPP Fluorescent Detection Set to form a fluorescent precipitate of HNPP (2-hydroxy-3-naphthoic acid-2’-phenylanilide phosphate) and Fast Red TR at the site of hybridization. Includes: In situ hybridization with DIG-labeled probes; Detection of DIG-labeled probes; Fluorescence microscopy. - [Read DNA In Situ Hybridization with an Alkaline Phosphatase-Based Fluorescent Detection System]

DNA In Situ Hybridization with an Alkaline Phosphatase-Based Fluorescent Detection System Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_124-126.pdf

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Protocol describes a high sensitivity indirect detection procedure for DIG-labeled hybridization probes. The procedure uses the components of the HNPP Fluorescent Detection Set to form a fluorescent precipitate of HNPP (2-hydroxy-3-naphthoic acid-2’-phenylanilide phosphate) and Fast Red TR at the site of hybridization. This procedure can be used to detect single copy sequences as small as 1 kb on human metaphase chromosomes. - [Read DNA In Situ Hybridization with an Alkaline Phosphatase-Based Fluorescent Detection System Protocol]

DNA Precipitation and Hybridization (CGH) Protocol - http://www.riedlab.nci.nih.gov/publications/DNA%20Precip%20&%20Hybrid.pdf

Category: Cytogenetics Protocols

Protocol for DNA precipitation and hybridization (CGH). - [Read DNA Precipitation and Hybridization (CGH) Protocol]

DNA Precipitation and Hybridization FISH Protocol - http://www.riedlab.nci.nih.gov/publications/DNA%20Precip%20&%20Hybrid%20FISH.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for DNA precipitation and hybridization (FISH). Includes: Precipitation; Slide Denaturation and Hybridization. - [Read DNA Precipitation and Hybridization FISH Protocol]

DNA preparation by cryotom tissue dissection - http://amba.charite.de/cgh/protocol/02/mapeng02.htm

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

DNA preparation by cryotom tissue dissection for CGH Comparative Genomic Hybridization. Institute of Pathology "Rudolf- Virchow-Haus" University Hospital CharitÃ© - [Read DNA preparation by cryotom tissue dissection]

Dot and Slot Hybridization of Purified RNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4052

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for dot and slot hybridization of purified RNA. Dot blotting of RNA is best carried out using purified preparations of RNA that are denatured with glyoxal or formaldehyde immediately before loading onto a nylon membrane through a vacuum manifold. - [Read Dot and Slot Hybridization of Purified RNA Protocol]

EMSA Protocol using 32-p. - http://www.well.ox.ac.uk/flint/EMSA.htm

Category: DNA Protocols: EMSA DNA-Protein Protocols

Primer design, Primer annealing, Primer labelling, Probe purification, In vitro hybridization DNA-protein Binding reaction. Jonathan Flint. - [Read EMSA Protocol using 32-p.]

FISH Hybridization Protocol - http://www.sanger.ac.uk/HGP/methods/cytogenetics/hybridisation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

FISH Hybridization Protocol. Sanger Institute. - [Read FISH Hybridization Protocol]

FISH Protocols for Drosophila - http://www.utoronto.ca/krause/FISH.html

Category: Drosophila Protocols: Drosophila Genetics Protocols

FISH protocols for Drosophila. Includes: RNA Probe Preparation; Embryo Collection and Fixation; Single FISH on Drosophila embryos; Post-Fixation, Hybridization and Post-Hybridization Washes; Development of FISH Signal; Storage, Mounting and Viewing of Samples; Double FISH on Drosophila Embryos; RNA-Protein Double Labeling; FISH on Dissected Tissues. - [Read FISH Protocols for Drosophila]

Fluorescence In Situ Hybridization (FISH) for DNA Replication Origins Protocol - http://www.natureprotocols.com/2006/10/27/fluorescence_in_situ_hybridiza.php

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for fluorescence in situ hybridization (FISH) for DNA replication origins. Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique used for the detection of specific chromosomal rearrangements and applicable to many different specimen types. FISH is widely used for several diagnostic applications. - [Read Fluorescence In Situ Hybridization (FISH) for DNA Replication Origins Protocol]

Fluorescence In Situ Hybridization (FISH) on Human Chromosomes and Interphase Nuclei Protocol - http://users.ugent.be/~fspelema/neubla/protocol/fish.htm

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for fluorescence in situ hybridization (FISH) on human chromosomes and interphase nuclei. Includes: Plasmid probes; COSMID-, YAC- and LIBRARY PROBES; Probe detection. - [Read Fluorescence In Situ Hybridization (FISH) on Human Chromosomes and Interphase Nuclei Protocol]

Fluorescence In Situ Hybridization of a Repetitive DNA Probe to Human Chromosomes in Suspension - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_104-107.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for fluorescence in situ hybridization of a repetitive DNA probe to human chromosomes in suspension. Hybridization technique which does not need formamide and dextran sulfate. As a model system, we used the repetitive specific human DNA probe pUC 1.77, labeled it with digoxigenin-11-dUTP by nick-translation, and hybridized it to metaphase chromosomes in suspension. These chromosomes were isolated by standard techniques from human lymphocytes. - [Read Fluorescence In Situ Hybridization of a Repetitive DNA Probe to Human Chromosomes in Suspension]

Fluorescence in situ Hybridization Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/fish.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols

Protocol for fluorescence in situ hybridization. - [Read Fluorescence in situ Hybridization Protocol]

Human DNA Microarray Hybridization - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_human.html

Category: DNA Microarray Protocols

Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation Washing and Scanning Arrays. Brown Lab. - [Read Human DNA Microarray Hybridization]

Hybridization and Detection Using the HC ExpressArray Kit Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4122

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol describes the direct detection of RNA on DNA microarrays using Hybrid Capture (HC) technology and the HC ExpressArray Kit developed by Diagene. The kit uses a proprietary antibody that binds specifically to RNA:DNA hybrids and a second, fluorescently labeled, antibody that detects the primary antibody. Total RNA is applied directly to a glass-spotted DNA microarray, and stable RNA:DNA hybrids are visualized via a Cy3-labeled secondary antibody. - [Read Hybridization and Detection Using the HC ExpressArray Kit Protocol]

Hybridization of Arrays - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_yeast.html

Category: DNA Microarray Protocols

Hybridization of Arrays. . General/Yeast Microarrays. Brown Lab - [Read Hybridization of Arrays]

Hybridization of Bacteriophage DNA on Filters Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3983

Category: Viral Manipulation Protocols: Phage Protocols

Using hybridization, it is possible to identify a single recombinant that carries the desired target sequence on a filter that carries the imprint of 15,000 or more plaques. - [Read Hybridization of Bacteriophage DNA on Filters Protocol]

Hybridization of Oligonucleotide Probes in Aqueous Solutions Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3883

Category: Nucleic Acid Modification Protocols: Nucleic Acid Labeling Protocols

Hybridization is carried out in conventional aqueous solvents at a temperature well below the predicted melting temperature. Nonspecific hybrids are then removed by washing at high stringency in buffers containing quaternary salts. Tetramethylammonium chloride (TMACl) is used with probes that are 14-50 nucleotides in length, whereas tetraethylammonium chloride (TEACl) is used with longer oligonucleotides. - [Read Hybridization of Oligonucleotide Probes in Aqueous Solutions Protocol]

Hybridization Protocol for Hybridizing fluorescent cDNA to slide - http://research.nhgri.nih.gov/microarray/hybridization.shtml

Category: DNA Microarray Protocols

Hybridization Protocol for Hybridizing fluorescent cDNA to slide. - [Read Hybridization Protocol for Hybridizing fluorescent cDNA to slide]

Hybridization SKY Protocol - http://www.riedlab.nci.nih.gov/publications/Hybridization%20%28SKY%29.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for hybridization (SKY). - [Read Hybridization SKY Protocol]

Hybridization to a Scanning Array Made on Glass Protocol - http://fds.oup.com/www.oup.co.uk/pdf/pas/6-5-7.pdf

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol for hybridization to a scanning array made on glass. - [Read Hybridization to a Scanning Array Made on Glass Protocol]

Articles (1-2 of 2)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

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