User Panel

My Panel

Bookmark Science Articles

hybridization Protocols

Search results for: hybridization

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

96-well RNA In Situ Hybridization Protocol - http://www.fruitfly.org/about/methods/RNAinsitu.html

Category: RNA Protocols: cDNA Libraries Library

96-well RNA In Situ Hybridization Protocol. Berkeley Drosophila Genome Project - [Read 96-well RNA In Situ Hybridization Protocol]

96-well RNA In Situ Hybridization Protocol - http://www.fruitfly.org/about/methods/RNAinsitu.html

Category: PCR Protocols: In-situ PCR Protocols

96-well RNA In Situ Hybridization Protocol. Probe Preparation, Cell Inoculation, PCR, RNA Probe Preparation, etc, Very In-depth Protocol and Method Conditions. Berkeley Drosophila Genome Project. - [Read 96-well RNA In Situ Hybridization Protocol]

A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis - http://www.tigr.org/tdb/microarray/conciseguide.html

Category: DNA Microarray Protocols

Hegde et al., Array Fabrication, microarray Printing, Probe Preparation and Hybridization, RNA Extraction, RNA labeling, Data Collection, Normalization, and Analysis.Institute for Genomic Research, Rockville, MD - [Read A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis]

A Concise Guide to cDNA Microarray Analysis â€“ II PDF. - http://pga.tigr.org/PDF/BiotechniquesCookbook_II.pdf

Category: DNA Microarray Protocols

Micro Array Fabrication, Probe Preparation and Hybridization, and Data Collection, Normalization and Analysis. Hegde, et al. biotechniques 2000. - [Read A Concise Guide to cDNA Microarray Analysis â€“ II PDF.]

A protocol for application of Polymerase Chain Reaction (PCR) in situ hybridization for detection of - http://www.im.microbios.org/03setember98/08%20Sterflinger.pdf

Category: PCR Protocols: In-situ PCR Protocols

A protocol for application of Polymerase Chain Reaction (PCR) in situ. hybridization for the detection of hyphomycetes. Sterflinger et al 1998. - [Read A protocol for application of Polymerase Chain Reaction (PCR) in situ hybridization for detection of]

Anatomy of a Comparative Gene Expression Study - http://www.cs.wustl.edu/~jbuhler//research/array/

Category: Genetics and Genomics: Cancer Genetics Protocols

Anatomy of a comparative gene expression study. Includes: Choosing Cell Populations; mRNA Extraction and Reverse Transcription; Fluorescent Labeling of cDNA's; Hybridization to a DNA Microarray; Scanning the Hybridized Array; Interpreting the Scanned Image. - [Read Anatomy of a Comparative Gene Expression Study]

Anatomy of a Comparative Gene Expression Study - http://www.cs.wustl.edu/~jbuhler//research/array/

Category: Microarray Protocols: Microarray Analysis Protocols

Information on anatomy of a comparative gene expression study. Includes: Choosing cell populations; mRNA Extraction and Reverse Transcription; Fluorescent labeling of cDNA's; Hybridization of DNA microarray; Scanning the hybridized array; Interpreting the scanned image. - [Read Anatomy of a Comparative Gene Expression Study]

aRNA Labeling and Hybridization Protocol - http://www.stanford.edu/group/sjeffreylab/PDF%20Files/aRNA_labeling.pdf

Category: Microarray Protocols: Microarray Analysis Protocols

Protocol for aRNA labeling and hybridization. - [Read aRNA Labeling and Hybridization Protocol]

BAC-FISH Protocol - http://www.nhm.ac.uk/hosted_sites/schisto/protocols/BAC_FISH.html

Category: BACs Bacterial Artificial Chromosome Protocols

Protocol uses the BIOPRIME reaction kit from GibcoBRL to prepare biotin-labelled BAC DNA which is detected using FITC-Avidin (Vector Labs, DCS grade). Reagents from other manufacturers may work equally well but have not been tested. Includes: Labeling of BAC clones; Ethanol precipitation; Hybridization; Post-hybridisation treatment / detection. - [Read BAC-FISH Protocol]

CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA Protocol - http://waldman.ucsf.edu/Protocols/indirectcgh.html

Category: Cytogenetics Protocols

Protocol for CGH of biotin labeled DNA vs digoxigenin labeled DNA. Includes: Reprecipitate DNA's; Denature slides; Hybridization; Washing and staining. - [Read CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA Protocol]

CGH Protocols - http://amba.charite.de/cgh/protocol/02/prot02.html

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

A nice map linking to protocols for CGH Comparative Genomic Hybridization. Hybridization protocols, detection and analysis. Institut fÃ¼r Pathologie University Hospital CharitÃ© - [Read CGH Protocols]

CGH Protocols PDF - http://www.bio.davidson.edu/projects/gcat/CGH/AJRyan_Method.pdf

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

Jackie Ryan, CGH Protocols. Univ Davidson. Excellent Detailed CGH procedure. Tagged DNA Hybridization, Post Hybridization Wash, Post 3DNA Hybridization Wash. Fragmentation. - [Read CGH Protocols PDF]

Choosing the Right Labeling Method for your Hybridization Experiment - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_15-18.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

Choosing the right labeling method for your hybridization experiment. Includes: Homogeneous labeling methods for DNA; Homogeneous labeling methods for RNA; Stability of probe-target interaction; Nonradioactive labeling of oligonucleotides; Double-stranded versus single-stranded probes. - [Read Choosing the Right Labeling Method for your Hybridization Experiment]

Chromosome Preparation and Fluorescence In-Situ Hybridization (FISH) Protocol - http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=18

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for chromosome preparation and Fluorescence in-situ hybridization (FISH). Chromosome preparation and Fluorescence in-situ hybridization (FISH). - [Read Chromosome Preparation and Fluorescence In-Situ Hybridization (FISH) Protocol]

Colony Hybridization Protocol - http://cmgm.stanford.edu/~vesicle/protocols/hybrid.html

Category: RNA Protocols: cDNA Libraries Library

Methods and procedure for Colony Hybridization. Vesicle Trafficking Home. - [Read Colony Hybridization Protocol]

Colony Hybridization Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/ColonyHybridization.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for colony hybridization. - [Read Colony Hybridization Protocol]

Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative - http://info.med.yale.edu/genetics/ward/tavi/CCKmethod.html

Category: Cytogenetics Protocols

Protocol for color changing karytoping (CCK): an M-FISH/SKY alternative. Includes: Slide preparation and hybridization; Probes, dyes and antibodies; Probe detection and image capture; Image processing. - [Read Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative]

Combined DNA In Situ Hybridization and Immunocytochemistry Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_127-134.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

Protocol for combined DNA in situ hybridization and immunocytochemistry for the simultaneous detection of nucleic acid sequences, proteins, and incorporated BrdU in cell preparations. Includes: Cell preparations and BrdU labeling; Detection of antigen by immunocytochemistry (ICC); Visualization of ICC antigen; -Gal-BCIG reaction (for producing a blue precipitate visible under brightfield microscopy); Cell processing for in situ hybridization; In situ hybridization (ISH); etc... - [Read Combined DNA In Situ Hybridization and Immunocytochemistry Protocol]

Combined LacZ staining and in situ hybridization PDF - http://www.oup.co.uk/pdf/pas/13-3-9.pdf

Category: Transgenic Mouse Protocols: Mouse Genotyping Protocols

Practical Approach Online, Oxford University Press. Combined LacZ staining and in situ hybridization of mouse embryos, beta-galactosidase. - [Read Combined LacZ staining and in situ hybridization PDF]

COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL - http://users.ugent.be/~fspelema/neubla/protocol/cgh.htm

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

SAVING OF DAPI-IMAGES, SLIDE PRETREATMENT, PROBE PREPARATION, PROBE DETECTION, washing blocking detection, Counterstaining with DAPI. Institute of Pathology,Humboldt-University of Berlin - [Read COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL]

Comparative Genomic Hybridization (CGH) Protocol - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/DNA/CGH.html#Protocol

Category: Cytogenetics Protocols

Protocol for comparative genomic hybridization (CGH). Includes: Theory of CGH; Sensitivity; Applications of CGH. - [Read Comparative Genomic Hybridization (CGH) Protocol]

Comparative Genomic Hybridization PDF - http://www.utoronto.ca/cancyto/protocols_software/CGH_protocol.pdf

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

Excellent Detailed CGH Comparative Genomic Hybridization Protocol. Squire Lab, Univ. Toronto. Includes: Labeling, Gel Interpretation, Slide Pre-Treatment and Denaturation, Probe Denaturation, Hybridization and Post Hybridization Washes and Detection, Tips - [Read Comparative Genomic Hybridization PDF]

Detection of Chromosomal Imbalances Using DOP-PCR and Comparative Genomic Hybridization (CGH) - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_95-103.pdf

Category: Cytogenetics Protocols

CGH provides a comprehensive picture of all chromosomal gains and losses within a single experiment. In contrast to banding analysis, CGH requires no preparation of metaphase chromosomes from the cells to be analyzed (which in certain tumor types may be difficult or even impossible). - [Read Detection of Chromosomal Imbalances Using DOP-PCR and Comparative Genomic Hybridization (CGH)]

Detection of Chromosomal Imbalances using DOP-PCR and Comparative Genomic Hybridization (CGH) - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_95-103.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for the Detection of chromosomal imbalances using DOP-PCR and comparative genomic hybridization (CGH). Includes: Preparation and labeling of DOP-PCR products; Comparative genomic hybridization (CGH). - [Read Detection of Chromosomal Imbalances using DOP-PCR and Comparative Genomic Hybridization (CGH)]

Detection of Even-Skipped Transcripts in Drosophila Embryos with PCR/DIG-Labeled DNA Probes Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_216-219.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for detection of even-skipped transcripts in drosophila embryos with PCR/DIG-labeled DNA probes. This protocol has been used to detect the transcript distribution of a number of genes by in situ hybridization, including evenskipped and seven-up, in whole mount Drosophila embryos, and engrailed Antennapedia in whole mount grasshopper embryos. Includes: Probe labeling; Evaluation of labeling reaction; Preparation of embryos, hybridization and detection. - [Read Detection of Even-Skipped Transcripts in Drosophila Embryos with PCR/DIG-Labeled DNA Probes Protocol]

Articles (1-2 of 2)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

[1-2]

Items 1-25 out of 125 displayed.

First [1] 2 3 4 5 Next Last

Total records: 125