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Sorting Transfected Cells Based on Gene Expression, Followed by Culture in Vivo - http://www.bdbiosciences.com/pdfs/whitePapers/23-2787-02.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

Describes how FACSort can be used to enrich for transfected mouse cells expressing high levels of the human thrombin receptor. The sorted fraction can then be cultured in vivo and reanalyzed 12 days later to show that it remains enriched for thrombin receptor-expressing cells. - [Read Sorting Transfected Cells Based on Gene Expression, Followed by Culture in Vivo]

Splitting Human ES cells on MEFs Protocol - http://ink.primate.wisc.edu/~thomson/pdfs/splitting_es.pdf

Category: Stem Cell Protocols: Mouse Embryonic Fibroblasts Protocols

Protocol for splitting human ES cells on MEFs. - [Read Splitting Human ES cells on MEFs Protocol]

Subcloning of Yeast Artificial Chromosomes into Phage Lambda Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast9.html

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol for the subcloning of Yeast artificial chromosome into phage lambda. To subclone the large insert fragment of human DNA contained within a YAC into a bacteriophage lambda vector. The subclones are 15 to 23 kb in size, and can be used to identify new polymorphic markers from a known region of the genome, to map a specific locus, and/or to screen other libraries. - [Read Subcloning of Yeast Artificial Chromosomes into Phage Lambda Protocol]

Summary of Methods using Pre-formed Iodixanol Gradients for the Purification of Virsues. - http://www.axis-shield.com/densityhome/optiprep/M17.pdf

Category: Cell Biology and Cell Culture

Summary of the techniques used for the purification of viruses using pre-formed iodixanol gradients. Viruses purified include: Semliki Forest, Retrovirus (from human melanoma cells), Rabies, Polyoma, Papillomavirus, Lassa, Influenza, Bovine viral diarrhea. - [Read Summary of Methods using Pre-formed Iodixanol Gradients for the Purification of Virsues.]

Thawing Human ES Cells Protocol - http://ink.primate.wisc.edu/~thomson/pdfs/thawing_es.pdf

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Protocol for thawing human ES cells. - [Read Thawing Human ES Cells Protocol]

Transient Transfection Into 293T Cells Protocol - http://www.cbrinstitute.org/labs/springer/protocols/jun_293T.html

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1A gene product. 293T is a derivative which also express SV40 large T antigen, allowing episomal replication of plasmids containing the SV40 origin and early promoter region. They (both) have the unusual property of being highly transfectable. - [Read Transient Transfection Into 293T Cells Protocol]

Use of PCR for Quality Control of a Genomic DNA Library Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4138

Category: DNA Protocols: Genomic DNA Library Protocols

Protocol describes the use of PCR to analyze the quality of a human genomic DNA library. It has been optimized for a human genomic DNA library containing fragments between approximately 50 and 500 bp. - [Read Use of PCR for Quality Control of a Genomic DNA Library Protocol]

Viral Detection and Concentration in Food - http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf

Category: Viral Protocols

Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods. - [Read Viral Detection and Concentration in Food]

Articles (1-3 of 3)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.