hormones Protocols

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Intraperitoneal (IP) Injection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4352

Category: Mouse Protocols: Mouse Surgical Procedures Protocols

Protocol describes intraperitoneal (IP) injection, which is the typical means of introducing most compounds, such as hormones and anesthetics, into the mouse. - [Read Intraperitoneal (IP) Injection Protocol]

Mouse Castration Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4387

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes castration of male mice, which is used to eliminate testicular hormones and/or to obtain testes for analysis without sacrificing the male. Castration is performed in a similar manner to vasectomy. - [Read Mouse Castration Protocol]

Multiplexing Cell Viability Assays Protocols - http://www.promega.com/paguide/chap4.htm#title6

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The latest generation of Promega cell-based assays includes luminescent and fluorescent chemistries to measure markers of cell viability, cytotoxicity and apoptosis, as well as to perform reporter analysis. Using these tools researchers can investigate how cells respond to growth factors, cytokines, hormones, mitogens, radiation, effectors, compound libraries and other signaling molecules. The protocols provided are guidelines for multiplexing cell-based assays & are intended as starting points. - [Read Multiplexing Cell Viability Assays Protocols]