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Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

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Antigen Retrieval for Immunohistochemistry with Paraffin-Embedded Tissues Protocol - http://www.animal.ufl.edu/hansen/Protocols/Immunohistochemistry_paraffin.htm

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Protocol used to for immunohistochemistry on paraffin-embedded sections. Based on use of microwave energy to effect antigen retrieval. The immunohistochemistry procedure, is for use of Biomeda's HistoScan kit based on a streptavidin-peroxidase/biotinylated second antibody detection system with 3-amino, 9-ethylcarbazole (AEC) as chromogen. Undoubtedly, other kits or home-made reagents will also work . - [Read Antigen Retrieval for Immunohistochemistry with Paraffin-Embedded Tissues Protocol]

Cartilage Staining of Xenopus tropicalis Protocol - http://tropicalis.berkeley.edu/home/gene_expression/cartilage-stain/alcian.html

Category: Xenopus Protocols

Protocol describes the use of a basic water-based dye to stain for acid mucosubstances and acetic mucins located on the cartilage of fixed embryos, permitting the examination of cartilage formation. - [Read Cartilage Staining of Xenopus tropicalis Protocol]

Colony Hybridization Protocol - http://cmgm.stanford.edu/~vesicle/protocols/hybrid.html

Category: RNA Protocols: cDNA Libraries Library

Methods and procedure for Colony Hybridization. Vesicle Trafficking Home. - [Read Colony Hybridization Protocol]

De-Jellying Eggs Protocol - http://tropicalis.berkeley.edu/home/manipulate_embryos/dejelly.html

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol for de-jellying eggs. - [Read De-Jellying Eggs Protocol]

Differences in Injection of X. laevis and X. tropicalis - http://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/differences.html

Category: Xenopus Protocols: Xenopus Injection Protocols

Differences in injection of X. laevis and X. tropicalis. Includes: X. tropicalis lays eggs about 4 hours after a boost of hCG; In vitro fertilization is not nearly as efficient in trops compared to laevis; X. tropicalis embryos are much softer than X. laevis embryos; X. tropicalis embryos whose jelly coats are removed by cysteine have a loose sticky vitelline membrane; X. tropicalis do not have a "summer slump". - [Read Differences in Injection of X. laevis and X. tropicalis]

DIG Labeling of cDNA Probes Protocol - http://home.cc.umanitoba.ca/~frist/Protocols/arrays/DIG_probes.html

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for DIG labeling of cDNA probes. Includes: Incorporation DIG during PCR; Estimating the yield of DIG labeled probes; DNA Dot Blotting; Prehybridization and Hybridization. - [Read DIG Labeling of cDNA Probes Protocol]

DIG Labeling of cDNA Probes Protocol - http://home.cc.umanitoba.ca/~frist/Protocols/arrays/DIG_probes.html

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Diseases of Xenopus tropicalis - http://tropicalis.berkeley.edu/home/husbandry/disease.html

Category: Xenopus Protocols

Information on the diseases of Xenopus tropicalis. Includes: PREMETAMORPHS: Bacterial Infection of Embryos; Protozoal Infestation of Tadpoles; POSTMETAMORPHS: Mycobacteriosis; Chytridiomycosis (Batrachochytrium dendrobatidis). - [Read Diseases of Xenopus tropicalis]

ENU Mutagenesis Protocol - http://tropicalis.berkeley.edu/home/manipulate_embryos/enu.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for ENU mutagenesis. - [Read ENU Mutagenesis Protocol]

Fixation and Fixatives - A great guide - http://home.primus.com.au/royellis/fix.htm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Fixation is to preserve cells and tissue constituents in as close a life-like state as possible and to allow them to undergo further preparative procedures without change. Fixation arrests autolysis and bacterial decomposition and stabilises the cellular and tissue constituents so that they withstand the subsequent stages of tissue processing. Great detailed guide with protocols. Anthony S-Y Leong. - [Read Fixation and Fixatives - A great guide]

Fixation of Xenopus Embryos - http://tropicalis.berkeley.edu/home/manipulate_embryos/fixation.html

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol for the fixation of xenopus embryos. - [Read Fixation of Xenopus Embryos]

Home-made Taq Polymerase Purification - http://www.nottingham.ac.uk/~mbzspd/methods/Home_made_Taq_polyme.html

Category: PCR Protocols

PCR polymerase costs can be high. If you are willing to work, you can produce bacteria containing the clone. It appears to produce lots of Taq and is quite stable. The proceedure takes 4 days start to (15 000 units of Taq) finish. The Taq also appears ver - [Read Home-made Taq Polymerase Purification]

Isolating Xenopus Genomic DNA Protocol - http://tropicalis.berkeley.edu/home/genetic_techniques/genomicDNA.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for isolating Xenopus genomic DNA. - [Read Isolating Xenopus Genomic DNA Protocol]

Karyotyping Xenopus Embryos Protocol - http://tropicalis.berkeley.edu/home/genetic_techniques/karyotype.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for karyotyping Xenopus embryos. - [Read Karyotyping Xenopus Embryos Protocol]

Kitchen Culture Kits - Plant Tissue Culture Resources - http://www.home.turbonet.com/kitchenculture/tcinfo.htm

Category: Plant Biology Protocols: Plant Tissue Culture

Cryopreservation protocols for different kinds of tissue of banana ... Teaching Plant Tissue Culture: University of Liverpool ... - [Read Kitchen Culture Kits - Plant Tissue Culture Resources]

Laboratory Survival Manual - http://keats.admin.virginia.edu/lsm/home.html

Category: General Research Protocols and Methods

Laboratory Survival Manual. Lab Safety, First AID, Chemicals and Equipment. Vaughn C. Kowahl - [Read Laboratory Survival Manual]

Large Scale Production of Monoclonal Antibodies - http://users.path.ox.ac.uk/~scobbold/tig/mprod.html

Category: Antibody Protocols: Antibody Production Protocols

Large scale production of monoclonal antibodies. Includes: Strategies to minimize mAb production costs; A home-made hollow fibre bioreactor system. - [Read Large Scale Production of Monoclonal Antibodies]

LRSM Confocal Microscope Home Page - http://glinda.lrsm.upenn.edu/

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

LRSM Confocal Microscope Home Page. Information on use and how confocal microscopy works. - [Read LRSM Confocal Microscope Home Page]

Photosynthesis Measurement Protocol - http://caplter.asu.edu/home/protocols/showProtocol.jsp?id=23

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Comparison among residential patch transition types and landscape water use efficiency, photosynthesis measurement protocol. - [Read Photosynthesis Measurement Protocol]

TUNEL Staining Protocol for Xenopus - http://tropicalis.berkeley.edu/home/gene_expression/TUNEL/TUNEL.html

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol is effective in identifying apoptotic cells in embryos that are undergoing substantial apoptosis. - [Read TUNEL Staining Protocol for Xenopus]

Xenopus Gynogenesis Protocol - http://tropicalis.berkeley.edu/home/genetic_techniques/gynogenesis/gynogenesis.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus gynogenesis. - [Read Xenopus Gynogenesis Protocol]

Xenopus Microinjection Protocol - http://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/inject.html

Category: Xenopus Protocols: Xenopus Injection Protocols

Protocol for Xenopus microinjection. - [Read Xenopus Microinjection Protocol]

Xenopus Whole Mount In Situ Hybridization Protocols - http://tropicalis.berkeley.edu/home/gene_expression/in-situ/insitu.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocols for Xenopus whole mount in situ hybridization. Includes: Protocols for Making Antisense RNA probe (dig labelled); Protocols for In Situ Hyb. - [Read Xenopus Whole Mount In Situ Hybridization Protocols]