heterokaryons Protocols

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Hints and Precautions for the Care, Feeding and Breeding of Neurospora - http://www.fgsc.net/methods/perkins.html

Category: Fungi Protocols: Neurospora Protocols

Hints and precautions for the care, feeding and breeding of Neurospora. Includes: Crossing; Tips on handling ascospoes General Laboratory Practices; Stockkeeping; Mutagenesis and enrichment; Tips for encouraging colonial growth; Solutions and media; Handling Heterokaryons. - [Read Hints and Precautions for the Care, Feeding and Breeding of Neurospora]

How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab - http://www.fgsc.net/neurosporaprotocols/How%20to%20avoid%20pitfalls%20and%20keep%20out%20of%20trouble.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres. - [Read How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab]

How to Make Heterokaryons in Neurospora crassa - http://www.fgsc.net/neurosporaprotocols/How%20to%20make%20heterokaryons%20in%20Neurospora%20crassa.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to make heterokaryons in Neurospora crassa. - [Read How to Make Heterokaryons in Neurospora crassa]

How to Use Helper Strains for Maintaining and Crossing Handicapped Recessive Mutants - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20helper%20strains%20for%20maintaining%20and%20crossing%20handicapped%20recessive%20mutants,%20for%20forcing%20and%20resolving%20heterokaryons,%20and%20for%20determining%20heterokaryon%20compatibility

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use helper strains for maintaining and crossing handicapped recessive mutants, for forcing and resolving heterokaryons, and for determining heterokaryon compatibility. - [Read How to Use Helper Strains for Maintaining and Crossing Handicapped Recessive Mutants]