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Human Hepatocyte Culture Protocol - http://www.montp.inserm.fr/u632/PDF/Pichard2006%20MethMolBiol.pdf

Category: Cell Biology and Cell Culture: Hepatocyte Cell Culture

Primary culture of hepatocytes is an in vitro model widely used to ... protocols for cytochromes P450IIIA in vivo and in primary cultures of animal and ...Human Hepatocyte Culture Protocol - [Read Human Hepatocyte Culture Protocol]

Hydrodynamics-Based Gene Transduction Protocol - http://www.natureprotocols.com/2006/06/29/hydrodynamicsbased_gene_transd.php

Category: Gene Delivery Protocols

This highly efficient in vivo gene transduction technique for laboratory mice. Hepatocytes are most effectively transduced by tail vein injection of a large volume of DNA solution in a short time. Practice with the injection technique is necessary!!! - [Read Hydrodynamics-Based Gene Transduction Protocol]

In Vitro Prediction of the Maximum Tolerated Dose Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/66.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Liver Toxicity Assays Protocols

The results of cytotoxicity tests in primary cultures of rat hepatocytes and in MDBK and McCoy cells can be used to predict the in vivo 4-wk maximum tolerated dose in rats and dogs. A correlation between in vitro cytotoxicity, as measured in this system, and LD50 values in rats and mice has also been established. - [Read In Vitro Prediction of the Maximum Tolerated Dose Protocol]

Isolation of Rat Hepatocytes Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/20.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Liver Toxicity Assays Protocols

Collagenase perfusion of rat liver yields a hepatocyte suspension which may be exposed to test compounds in order to assess their effects on cell viability and enzyme leakage. - [Read Isolation of Rat Hepatocytes Protocol]

Preparation of primary cultures of rat hepatocytes (or freshly isolated hepatocytes) - http://www.surgery.usc.edu/divisions/hep/research-cellculture.html

Category: Cell Biology and Cell Culture: Hepatocyte Cell Culture

USC Liver Transplant Surgery Program and Center for Liver Disease. Preparation of primary cultures of rat hepatocytes (or freshly isolated hepatocytes). - [Read Preparation of primary cultures of rat hepatocytes (or freshly isolated hepatocytes)]

Rat Hepatocyte Flow Cytometric Cytotoxicitiy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/23.htm#TOP

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Liver Toxicity Assays Protocols

Flow cytometry is used to monitor drug-induced changes in DNA and protein contents of hepatocytes cultured at physiological oxygen concentrations. - [Read Rat Hepatocyte Flow Cytometric Cytotoxicitiy Test]