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Single Strand DNA Prep For Sequencing Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S2.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for single strand DNA prep for sequencing. This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly). - [Read Single Strand DNA Prep For Sequencing Protocol]

Southern Blotting Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S5.html

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for southern blotting. - [Read Southern Blotting Protocol]

Southwestern Blot Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S3.html

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for southwestern blot. - [Read Southwestern Blot Protocol]

Testing Rabbit Bleeds By Elisa - http://mitchison.med.harvard.edu/protocols/ab2.html

Category: Immunology

Coupling Peptide to BSA, ELISA with Peptide conjugated to BSA. Claire Walczak - [Read Testing Rabbit Bleeds By Elisa]

Transient Transfection Protocol: Viral Production - http://genetics.med.harvard.edu/~cepko/protocol/mike/V2.html

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection protocol for the ecotropic/amphotropic producer line including titering and concentration of viral stocks. - [Read Transient Transfection Protocol: Viral Production]

Transient Transfection: Viral Production - http://genetics.med.harvard.edu/~cepko/protocol/mike/V1.html

Category: Transfection Protocols: Transient Transfection Protocols

This transient transfection protocol for the ecotropic/amphotropic producer line includes instructions for titering and concentration of viral stocks. This procedure gives consistently higher transfection efficiency. - [Read Transient Transfection: Viral Production]

Tubulin Polymerization with GTP/GMPCPP/Taxol Protocol - http://mitchison.med.harvard.edu/protocols/poly.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for tubulin polymerization with GTP/GMPCPP/ Taxol. Includes: Solutions & Supplies; Prepolymerization Clarification; GTP Polymerization; Taxol Polymerization; GMPCPP Polymerization; Determining Concentration of GMPCPP/Taxol MTs. - [Read Tubulin Polymerization with GTP/GMPCPP/Taxol Protocol]

Tyramide Amplification and Synthesis Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/T4.html

Category: Immunohistochemistry Protocols

Protocol provides the sensitivity of enzymatic detection (HRP) in immunohistochemical procedures with the versatility and convenience of fluorescence detection. - [Read Tyramide Amplification and Synthesis Protocol]

Western Blot Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/W1.html

Category: Western Blot Protocols: General Western Blot Methods Protocols

This protocol was developed for the BIORAD protein gel and transfer apparatus. The buffers can be used with any electrophoresis/transfer system. - [Read Western Blot Protocol]

[3H]Thymidine Labeling Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/T3.html

Category: Cell Culture Protocols

Protocol on [3H]Thymidine labeling. Protocol includes: Explant Culture Medium; 100X (cold) Thymidine; Dektol Developer; Fixer - [Read [3H]Thymidine Labeling Protocol]

Articles (1-2 of 2)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

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