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aCGH Labeling Protocol - http://genomic.dfci.harvard.edu/aCGHLabelingChecklistv1_9.pdf

Category: Cytogenetics Protocols: Array CGH

aCGH Labeling Protocol. Harvard University. - [Read aCGH Labeling Protocol]

Antibody Purification - http://axon.med.harvard.edu/~cepko/protocol/mike/A1.html

Category: Protein Protocols: Antibody Purification Protocols

This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography. Mike A. Dyer Lab Harvard. - [Read Antibody Purification]

Antigen Retrieval - Immunohistochemistry - http://axon.med.harvard.edu/~cepko/protocol/mike/A2.html

Category: Immunology: Antigen Retrieval

Denaturing Solution and Procedure for AR. Mike Dyer. - [Read Antigen Retrieval - Immunohistochemistry]

Antigen Retrieval Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/A2.html

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Protocol for antigen retrieval. - [Read Antigen Retrieval Protocol]

b-galactosidase Solution Assay Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/B2.html

Category: Immunohistochemistry Protocols

Protocol for b-galactosidase solution assay. - [Read b-galactosidase Solution Assay Protocol]

Baculovirus Production for Recombinant Proteins - http://axon.med.harvard.edu/~cepko/protocol/mike/B1.html

Category: Protein Protocols: Protein Expression Protocols

Baculovirus Production for Recombinant Proteins. Protocol is a modified version of the pharmingen Baculogold kit. Brian Dynlacht. Mike A. Dyer Lab. - [Read Baculovirus Production for Recombinant Proteins]

CHAPOL Lineage Analyis Protocol - http://genetics.med.harvard.edu/~cepko/protocol/CHAPOL.pdf

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Protocol for CHAPOL lineage analysis. - [Read CHAPOL Lineage Analyis Protocol]

CHO Centrosome Prep Protocol - http://mitchison.med.harvard.edu/protocols/mt1.html

Category: Cell Organelle Protocols: Nucleus Protocols

The key step is the lysis which solubilizes centrosomes away from nuclei by very low ionic strength lysis after treatment of cells with nocodazole and cytochalasin B. The released centrosomes are then centrifuged onto a Ficoll cushion (to avoid pelleting) and the interface between the lysate and the Ficoll is collected and the centrosomes are concentrated on a sucrose gradient. Fractions are assayed by spindown and double IF with 5051 serum and anti-tubulin and the pooled fractions are frozen... - [Read CHO Centrosome Prep Protocol]

Chromosomal DNA Preparation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C3.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Isolation from Mammalian Cells Protocols

Protocol for chromosomal DNA preparation. Protocol was developed for cultured cells but should be appropriate for dissociated tissues as well. - [Read Chromosomal DNA Preparation Protocol]

Conditioned Medium for Retinal Explant Culture Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C6.html

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: Neuronal Cell Culture Media Protocols

Protocol for conditioned medium for retinal explant culture. - [Read Conditioned Medium for Retinal Explant Culture Protocol]

COS Cell Transfection Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C5.html

Category: Transfection Protocols

COS cell transfection protocol. Includes solutions: DEAE Dextran and 1000X Chloroquine - [Read COS Cell Transfection Protocol]

Coupling Peptides To Resin For Affinity Purification - Claire Walczak. - http://mitchison.med.harvard.edu/protocols/ab3.html

Category: Peptide Protocols: Antibodies from Peptides Protocols

Coupling Peptides To Resin For Affinity Purification - Claire Walczak. - [Read Coupling Peptides To Resin For Affinity Purification - Claire Walczak.]

Crude Depletion Conditions for XKCM1 Protocol - http://mitchison.med.harvard.edu/protocols/ext1.html

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol for crude depletion conditions for XKCM1. - [Read Crude Depletion Conditions for XKCM1 Protocol]

Cryosectioning Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C7.html

Category: Immunohistochemistry Protocols: Cryosectioning Protocols

Protocol for cryosectioning. While the timing of the various steps in this protocol are probably not critical, process the tissue all at once to ensure that RNA and/or proteins do not get degraded. Includes: 20% Paraformaldehyde/4% Paraformaldehyde-PBS; Sucrose/PBS. - [Read Cryosectioning Protocol]

CsCl Prep of Plasmid DNA Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C1.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for CsCl prep of plasmid DNA. This is a standard large scale prep. For plasmid DNA which gives a yield of 0.5 1.0 mg. - [Read CsCl Prep of Plasmid DNA Protocol]

CSF Extract Prep for Xenopus Spindle Assembly Protocol - http://mitchison.med.harvard.edu/protocols/ext3.html

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol for CSF extract prep for Xenopus spindle assembly. - [Read CSF Extract Prep for Xenopus Spindle Assembly Protocol]

Detection of ß-Galactosidase and Alkaline Phosphastase Activities in Tissue Protocol - http://genetics.med.harvard.edu/~cepko/protocol/xgalplap-stain.htm

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for detection of ß-Galactosidase and alkaline phosphastase activities in tissue. - [Read Detection of ß-Galactosidase and Alkaline Phosphastase Activities in Tissue Protocol]

Dissociated Retina Immunohistochemistry Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D6.html

Category: Immunohistochemistry Protocols

Protocol for dissociated retina immunohistochemistry. - [Read Dissociated Retina Immunohistochemistry Protocol]

Dissociated Retinal Cell Scoring Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/score.html

Category: Immunohistochemistry Protocols

Protocol for dissociated retinal cell scoring. Includes: Rods; Cones; Bipolars; Müller Glial Cells; Pan-Amacrine/Horizontal. - [Read Dissociated Retinal Cell Scoring Protocol]

DNA Fragment Purification from Agarose or Acrylamide - http://axon.med.harvard.edu/~cepko/protocol/mike/D5.html

Category: DNA Protocols: DNA Extraction Protocols: DNA Extraction Polyacrylamide Gels

DNA Fragment Purification from Agarose or Acrylamide. The protocol for fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. - [Read DNA Fragment Purification from Agarose or Acrylamide]

DNA Fragment Purification from Agarose or Acrylamide Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D5.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for DNA fragment purification from agarose or acrylamide. For fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. - [Read DNA Fragment Purification from Agarose or Acrylamide Protocol]

DNA Fragment Purification from Agarose Protocol - http://axon.med.harvard.edu/~cepko/protocol/mike/D5.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

DNA Fragment Purification from Agarose Protocol. This protocol is best for fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. - [Read DNA Fragment Purification from Agarose Protocol]

DNA Plasmid Prep. Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D1.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for DNA plasmid prep. - [Read DNA Plasmid Prep. Protocol]

DnaseI Footprint Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/F2.html

Category: DNA Protein Interactions Protocols: Dnase Footprinting Assay Protocols

Protocol for DnaseI footprint. - [Read DnaseI Footprint Protocol]

dsRNA Synthesis Protocol - http://flyrnai.org/RNAi_FAQ_dsrna_syn.html

Category: siRNA and RNAi Protocols

dsRNA From Clones, Primer Designed dsRNA. Drosophila RNAi Screening Center at Harvard Medical School - [Read dsRNA Synthesis Protocol]

Articles (1-2 of 2)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

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