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Attaching Suspension Cells to Slides Using a Cytocentrifuge Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4291

Category: Cell Culture Protocols: Cell Staining Protocols

A simple method for handling suspension cells is to attach them to a solid substrate before fixation, and one way to do this is to use a cytocentrifuge. - [Read Attaching Suspension Cells to Slides Using a Cytocentrifuge Protocol]

Biomethodology of the Guinea Pig - http://research.uiowa.edu/animal/?get=g_pig

Category: Animal Research Techniques

Guinea Pig Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Guinea Pig]

Biomethodology of the Mouse - http://research.uiowa.edu/animal/?get=mouse

Category: Animal Research Techniques

Mus mouse Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Mouse]

Biomethodology of the Rabbit - http://research.uiowa.edu/animal/?get=rabbit

Category: Animal Research Techniques

Rabbit Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rabbit]

Biomethodology of the Rat - http://research.uiowa.edu/animal/?get=rat

Category: Animal Research Techniques

Rat Biology, Sources Ordering, Behavior, Basic Husbandry, Identification, Handling, Breeding, Restraint Devices, Sexing, Transport, Anesthesia, Euthanasia, Rodent Surgery, Blood Collection, Administration of Fluid. Univ. Iowa. Michael Parker. - [Read Biomethodology of the Rat]

CAT.INIST.FR - http://cat.inist.fr/?aModele=afficheN&cpsidt=16477965

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

We have established a high-throughput protocol for mouse genotyping using two robotics workstations: a liquid-handling robot to assemble PCR and a ... - [Read CAT.INIST.FR]

Cryogenic Preservation and Storage of Animal Cells Protocol - http://www.corning.com/Lifesciences/technical_information/techDocs/Cell_freezing_protocol.pdf

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

Cryogenic preservation (storage below -100°C) of cell cultures is widely used to maintain backups or reserves of cells without the associated effort and expense of feeding and caring for them. The success of the freezing process depends on four critical areas: Proper handling and gentle harvesting of the cultures; Correct use of the cryoprotective agent; A controlled rate of freezing; Storage under proper cryogenic conditions. - [Read Cryogenic Preservation and Storage of Animal Cells Protocol]

Ethics, Guidelines for Animal Research PDF - http://www.brl.uic.edu/courses/TNotes05.pdf

Category: Animal Research Techniques

Needs of animals, Ventilation, lighting and temperature considerations. Cage size/density. Handling. Blood Collection and guidlines. EXCELLENT GUIDE GC470 ESSENTIALS FOR ANIMAL RESEARCH BRL Univ. Illinois. - [Read Ethics, Guidelines for Animal Research PDF]

Growth of Primary Cell Culture and Viral Vector Handling Protocols - http://www.stanford.edu/dept/EHS/prod/researchlab/bio/docs/Palmer_Bio_protocol.pdf

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

The procedures involve the isolation and growth of primary cell cultures from rodent and human tissue as well as the use of viral vectors for the introduction and expression of mammalian genes in cells in culture and in live rodents. - [Read Growth of Primary Cell Culture and Viral Vector Handling Protocols]

Handling and Using Insertion Libraries - http://ygac.med.yale.edu/mtn/protocol.stm

Category: Yeast Protocols

Handling and using insertion libraries. Includes: Curing strains of endogenous 2-micron; mTn-lacZ/LEU2 insertion library; mTn-3xHA/lacZ insertion library; mTn-3xHA/GFP insertion library. - [Read Handling and Using Insertion Libraries]

Hints and Precautions for the Care, Feeding and Breeding of Neurospora - http://www.fgsc.net/methods/perkins.html

Category: Fungi Protocols: Neurospora Protocols

Hints and precautions for the care, feeding and breeding of Neurospora. Includes: Crossing; Tips on handling ascospoes General Laboratory Practices; Stockkeeping; Mutagenesis and enrichment; Tips for encouraging colonial growth; Solutions and media; Handling Heterokaryons. - [Read Hints and Precautions for the Care, Feeding and Breeding of Neurospora]

Isolation and handling of primary mouse embryonic fibroblasts (MEFs) - http://www.molgen.mpg.de/~rodent/MEF_protocol.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: MEF Protocols Mouse Embryonic Fibroblasts

Embryonic stem cells are usually grown on a layer of mitotically inactivated primary mouse embryonic fibroblasts to promote growth and prevent differentiation. Boris Greber - [Read Isolation and handling of primary mouse embryonic fibroblasts (MEFs)]

Laboratory Safety Manual - http://www.mcgill.ca/ehs/laboratory/labsafety/

Category: General Research Protocols and Methods: Laboratory Safety

Laboratory Safety Manual. McGill EHS. Workplace Hazardous Materials Information System (WHMIS), Control of Chemical Hazards, Handling and Storage of Laboratory Chemicals, Fire Safety in the Laboratory, Hazardous Waste Disposal. - [Read Laboratory Safety Manual]

Media and Culture of Yeast Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=E5192D47CE9B0776DB7D136C34ED148A&objectid=6673ABA6C2DB2E4E7564EDFA26589256

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

The yeast, Saccharyomyces cerevisiae, has become an important organism in molecular, biochemical, and genetic analysis. The organism has specific requirements for growth under a variety of conditions. The media, both liquid and solid, simple, define, and complex are describe in this unit. Also included are methods for handling, storing, and shipping stock of yeast. - [Read Media and Culture of Yeast Protocol]

Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorec - http://nar.oxfordjournals.org/cgi/content/full/33/5/e46

Category: DNA Microarray Protocols: DNA Methylation Microarray CpG

Kimura et al., NAR 2005. "easy-handling technology provided reproducible and precise measurement of methylated CpGs in MGMT promoter and, thus, our method may bring about a potential evolution in the handling of a variety of high-throughput DNA methylatio - [Read Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorec]

MICROARRAY cDNA CLONE GROWTH AND TEMPLATE - http://pga.tigr.org/sop/M001_1a.pdf

Category: DNA Microarray Protocols

This protocol describes clone handling, plate replication, and DNA template preparation in a 96 well format. Hasseman. TIGR Microarray Protocols - [Read MICROARRAY cDNA CLONE GROWTH AND TEMPLATE]

Peptide Handling and Storage - http://www.sigmaaldrich.com/Brands/Sigma_Genosys/Custom_Peptides/Key_Resources/Handling___Storage.html

Category: Peptide Protocols: Peptide Storage

In general, peptide solutions are stable for up to a week at 4Â°C. However, if the peptide sequence has inherent instability (Peptide Stability), it might be better to freeze the solution when not in use. Peptide solutions at pH>8 should also be frozen when not in use. Sigma Aldrich. - [Read Peptide Handling and Storage]

Peptide Handling Guides - http://www.sigmaaldrich.com/sigma/general%20information/peptide_handling_guide.pdf

Category: Peptide Protocols: Dissolving Handling Peptides

Peptide Handling Guides. Storage Guidelines for Lyophilized Peptides, Strategy for Dissolving Single Peptides, Determining Solubility Characteristics of peptides, Dissolving Approach for Charged Peptides, Dissolving Approach for Hydrophobic/Uncharged Peptides, Guidelines for Dissolving Several Peptides, Peptide Stability and Potential Degradation Pathways, and storage of peptides. Sigma Aldrich. PDF. - [Read Peptide Handling Guides]

Peptide Storage Recommendations - http://www.covalab.com/handling/peptide%20handling%20&%20storage.pdf

Category: Peptide Protocols: Peptide Storage

Peptide Storage Recommendations. CovaLab. - [Read Peptide Storage Recommendations]

Quantification of DNA Methylation in Electrofluidics Chips Protocol - http://www.natureprotocols.com/2006/06/23/quantification_of_dna_methylat.php

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Protocol for quantification of DNA methylation in electrofluidics chips. Describe Bio-COBRA, a modified protocol for Combined Bisulfite Restriction Analysis (COBRA), that incorporates an electrophoresis step in microfluidics chips. Microfluidics technology involves the handling of small amounts of liquid in miniaturized systems. - [Read Quantification of DNA Methylation in Electrofluidics Chips Protocol]

Restraint and Handling of Animals Guide - http://www.ahc.umn.edu/rar/handling.html

Category: Animal Research Techniques

Handling Methods for animals: mice, rats, hamsters, rabbits, cats, dogs, non-human primates, swine, goats, sheep, calves, pigs. Restraint devices, drug administration. - [Read Restraint and Handling of Animals Guide]

Static Culture of Postimplantation Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4373

Category: Cell Culture Protocols

Protocol describes static culture of postimplantation embryos, an alternative to the roller method. The static method is best suited to 6.0 to 7.0 days post coitum (dpc) embryos followed for 24 hours (7.0 dpc embryos) to 48 hours (6.0 dpc embryos) of development. It allows repetitive real-time observation with minimal handling of the embryo. It is especially useful if single or small groups of embryos need to be distinguished from each other. - [Read Static Culture of Postimplantation Embryos Protocol]

Tips for Sample Preparation for MALDI mass spectrometry - http://www.cfgbiotech.com/proteomics/sample_prep.htm

Category: Mass Spectrometry Protocols

Tips for Sample Preparation for MALDI mass spectrometry. Sample quantity, Solvent, Protein/Peptide sample handling. U. Albany. CFG - [Read Tips for Sample Preparation for MALDI mass spectrometry]

Unmasking Hidden Epitopes Using the Pressure Cooker Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4530

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

The principle behind the pressure cooker method described here is to use extended periods of heat to break some of the subcellular structures that block antibody access. This approach is appropriate for handling specimens on glass slides. The major advantages of the pressure cooker method are the ability to handle a large number of slides simultaneously, the convenience of using metal racks, and the avoidance of any hot spots that are found in the microwave. - [Read Unmasking Hidden Epitopes Using the Pressure Cooker Protocol]

Viral Vector Handling Information - http://www.stanford.edu/dept/EHS/prod/researchlab/bio/docs/Palmer_Bio_protocol.pdf

Category: Cloning Protocols: Vector Protocols

Information on how to handle viral vectors. Includes: Adenovirus; MoMLV retrovirus; MoMLV-based Retroviral Vectors: Base classification BSL-1. - [Read Viral Vector Handling Information]

Articles (1-8 of 8)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Acid Washing Microinjection Pipettes Protocol

Acid Washing of Glass microinjection pipettes protocol.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.