guidelines Protocols

Category: Cell Biology and Cell Culture: Troubleshooting Cell Culture Problems

Immunology Applications Core Facility, Univ. Chicago - [Read Cell Culture Guidelines]

Category: Animal Research Techniques

Needs of animals, Ventilation, lighting and temperature considerations. Cage size/density. Handling. Blood Collection and guidlines. EXCELLENT GUIDE GC470 ESSENTIALS FOR ANIMAL RESEARCH BRL Univ. Illinois. - [Read Ethics, Guidelines for Animal Research PDF]

Category: Immunology: T Cell Protocols

Describes methods for labeling high or low numbers of lymphocytes with CSFE. Protocols are provided to use CSFE-labeled cells in cell transfer studies or as cells to be cultured in vitro. Detailed guidelines for positioning of CSFE-labeled lymphocytes in lymphoid organs or other tissues are included for those wishing to use this approach to study lymphocyte migration. - [Read Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Long PCR Protocols

General Guidelines for Long PCR Conditions and Enzyme Mixtures. Pete Estep. Harvard. - [Read Long PCR Reagents and Guidelines]

Category: PCR Protocols: Quantitative PCR Protocols

General guidelines for long-PCR conditions and enzyme mixtures. Efficient long-PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Includes: For PCR with low-complexity templates (e.g., plasmid and cosmid inserts); For PCR with moderate-complexity templates (e.g., bacterial genomic DNA); For PCR with high-complexity templates (e.g., human genomic DNA). - [Read Long-PCR Reagents and Guidelines]

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

The culture medium is an essential component of the in vitro environment and must be selected or designed with care. This protocol provides guidelines for design of serum-containing and serum-free media, selective and specialty media, and media for growth under special conditions such as soft-agar growth. - [Read Media for Culture of Mammalian Cells Protocol]

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The latest generation of Promega cell-based assays includes luminescent and fluorescent chemistries to measure markers of cell viability, cytotoxicity and apoptosis, as well as to perform reporter analysis. Using these tools researchers can investigate how cells respond to growth factors, cytokines, hormones, mitogens, radiation, effectors, compound libraries and other signaling molecules. The protocols provided are guidelines for multiplexing cell-based assays & are intended as starting points. - [Read Multiplexing Cell Viability Assays Protocols]

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Describes the steps in detail to isolate and expand neural stem cells in the form of neurospheres from tissue dissections of the post-natal mouse brain. Procedures for the long term passage of neurospheres and the cryopreservation of neurospheres are also provided. In addition to the guidelines and tips for generating neurosphere cultures, we describe the method to prepare neurospheres for analysis by light microscopy. - [Read Neural Stem Cell Culture: Neurosphere Generation, Microscopical Analysis and Cryopreservation]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

PCR Tips and Tricks - General Guidelines and Troubleshooting PCR polymerase chain reaction. The Genomic Variation Laboratory, U.C Davis - [Read PCR Tips and Tricks]

Category: PCR Protocols: PCR Optimization

PCR Tips and Tricks - General Guidelines and Troubleshooting PCR polymerase chain reaction. The Genomic Variation Laboratory, U.C Davis - [Read PCR Tips and Tricks]

Category: Peptide Protocols: Dissolving Handling Peptides

Peptide Handling Guides. Storage Guidelines for Lyophilized Peptides, Strategy for Dissolving Single Peptides, Determining Solubility Characteristics of peptides, Dissolving Approach for Charged Peptides, Dissolving Approach for Hydrophobic/Uncharged Peptides, Guidelines for Dissolving Several Peptides, Peptide Stability and Potential Degradation Pathways, and storage of peptides. Sigma Aldrich. PDF. - [Read Peptide Handling Guides]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Long PCR Protocols

Long PCR Buffer, Cycle times and temperatures, Picking Primers, Hot Start Polymerases for Long PCR. Harvard-Lipper Center for Computational Genetics. Modified from Cheng et al. - [Read Reagents and Guidelines for Long PCR]

Category: Cell Biology and Cell Culture: Cell Culture Techniques

Tissue Culture Methods. Julie B. Wolf, Univ. Maryland. Protocols for Counting Cells, Preservation and storage of cell lines, maintenance of cell lines, media and growth, and safety guidelines for working with cells. - [Read Tissue Culture Methods]