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Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

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Near-Field Scanning Optical Microscopy: NSOM Probes - http://microscopy.fsu.edu/primer/techniques/nearfield/nearfieldprobes.html

Category: Microscopy Protocols: Optical Microscopy Protocols

Near-field scanning optical microscopy can achieve spatial resolution performance beyond the classical diffraction limit by employing a sub-wavelength light source or detector positioned in close proximity to a specimen. Such a sub-wavelength source usually consists of an aperture at the end of a tapered probe, which functions basically as a wave guide. Includes info.: Fiber Probe Fabrication; Pulling Method; Meniscus Etching; Selective Etching; Apertureless and Alternative Probe Designs etc. - [Read Near-Field Scanning Optical Microscopy: NSOM Probes]

PCR Amplification Manual Roche - http://www.roche-applied-science.com/PROD_INF/MANUALS/pcr_man/index.htm

Category: PCR Protocols

PCR Amplification Manual Roche. Guide to Standard PCR Protocl, Long PCR Protocol, Hot Start PCR, PCR and Cloning, Basic PCR, other. Roche Applied Science - [Read PCR Amplification Manual Roche]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

A Great PCR and Multiplex Guide. Topics for PCR optimization. Primer conditions, PCR temperatures, etc. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

PCR and Multiplex Guide. Amazing Guide to primer amount, MgCl2 amount, annealing temperature, and more. With picture examples. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: PCR Protocols: PCR Optimization

A Great PCR and Multiplex Guide. Topics for PCR optimization. Primer conditions, PCR temperatures, etc. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: PCR Protocols: Standard PCR Protocol

PCR and Multiplex Guide. Amazing Guide to primer amount, MgCl2 amount, annealing temperature, and more. With picture examples. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR Guide - Promega - http://www.promega.com/guides/pcr_guide/

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR Guide - Promega - [Read PCR Guide - Promega]

PCR Guide - Promega - http://www.promega.com/guides/pcr_guide/

Category: PCR Protocols

PCR Guide - Promega - [Read PCR Guide - Promega]

Polymerase Chain Reaction (PCR) Optimization - http://www.uaf.edu/museum/resumes/cook/mollab/pcr.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

Optimizing PCR Reaction Conditions. Optimizing PCR Tips and Guide. University of Alaska Museum - [Read Polymerase Chain Reaction (PCR) Optimization]

Principles and Applications of Liquid Scintillation Counting PDF - http://www.ehs.psu.edu/radprot/LSC_Theory2.pdf

Category: Radioactivity: Liquid Scintillation Counting

Excellent guide for Liquid Scintillation Counting. Includes protocols and methods for counting gel slices, SPECIAL SAMPLE PREPARATION PROTOCOLS: TLC Plates, protocol for Counting Samples on Cellulose-ester, Filters (MilliporeTM filters), Counting Tissue radioacitivity, Counting 14CO2, Samples in Polyacrylamide Gels. National Diagnostics. National Diagnostics Laboratory Staff. - [Read Principles and Applications of Liquid Scintillation Counting PDF]

Promega PCR Guide - http://www.promega.com/guides/pcr_guide/default.htm

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Promega PCR Guide - [Read Promega PCR Guide]

Promega PCR Guide - http://www.promega.com/guides/pcr_guide/default.htm

Category: PCR Protocols

Promega PCR Guide - [Read Promega PCR Guide]

Protein Expression Protocols and Applications Guide PDF - http://www.promega.com/paguide/chap5.pdf

Category: Protein Protocols: Protein Expression Protocols

Protein Expression Protocols and Applications Guide PDF. Promega. In vitro translation systems in Prokaryotes and Eukaryotes. Promega. - [Read Protein Expression Protocols and Applications Guide PDF]

Protocol Guide for the N. crassa Yeast Artificial Chromosome Library - http://www.fgsc.net/fgn41/yac1.html

Category: Fungi Protocols: Neurospora Protocols

Protocol guide for the N. crassa yeast artificial chromosome library. Includes: Chromosome Walking; Hybridization screening of the YAC library; YAC restriction mapping and contig building; Preparation of chromosomal DNA plugs of YAC clones; Partial restriction enzyme digestion of YAC DNA plugs; Using CHEF gel analysis to resolve YAC clones; Southern Hybridization; Isolation of terminal restriction fragments from cloned DNA inserts in YAC clones; etc. - [Read Protocol Guide for the N. crassa Yeast Artificial Chromosome Library]

Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures - http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvhpcr.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures. Informative Guide. Jack Vanden Heuvel. - [Read Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures]

Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures - http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvhpcr.html

Category: PCR Protocols: Standard PCR Protocol

Real-Time PCR Guide - Informative Background - http://dorakmt.tripod.com/genetics/realtime.html

Category: Real Time PCR

Real-Time PCR Guide - Informative Background. M.Tevfik Dorak. - [Read Real-Time PCR Guide - Informative Background]

Restraint and Handling of Animals Guide - http://www.ahc.umn.edu/rar/handling.html

Category: Animal Research Techniques

Handling Methods for animals: mice, rats, hamsters, rabbits, cats, dogs, non-human primates, swine, goats, sheep, calves, pigs. Restraint devices, drug administration. - [Read Restraint and Handling of Animals Guide]

Restriction Enzyme Troubleshooting Guide - http://www.fermentas.com/techinfo/re/troubleshoot.htm

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Restriction Enzyme Troubleshooting Guide. Fermentas. Problems: Undigested or incompletely digested DNA, additional bands atypical, Diffused DNA zones, Low fragment ligation efficiency. - [Read Restriction Enzyme Troubleshooting Guide]

Rotor / Adapter Selection Guide - http://www.bdbiosciences.com/discovery_labware/technical_resources/labtools/p42.pdf

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Rotor / Adapter Selection Guide. BD Biosciences. - [Read Rotor / Adapter Selection Guide]

Sporulation Guide - http://www.genomics.princeton.edu/dunham/sporulationdissection.htm

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocols and Information on sporulation. Includes: SPO++ media; 10X amino acid stock; Sporulation; Dissection. - [Read Sporulation Guide]

Standard Ligation - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/molecularCloning/standardLigation.html

Category: Molecular Biology Protocols: DNA Ligation Protocols

Nice guide for DNA ligation reaction. Standard Ligation for DNA. Schneitz Lab. - [Read Standard Ligation]

Standard PCR Protocol - http://www.mcb.uct.ac.za/pcrcond.htm

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Standard PCR Protocol Guide. Ed Rybicki. - [Read Standard PCR Protocol]