guide Protocols

Category: DNA Microarray Protocols

Hegde et al., Array Fabrication, microarray Printing, Probe Preparation and Hybridization, RNA Extraction, RNA labeling, Data Collection, Normalization, and Analysis.Institute for Genomic Research, Rockville, MD - [Read A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis]

Category: DNA Microarray Protocols

Micro Array Fabrication, Probe Preparation and Hybridization, and Data Collection, Normalization and Analysis. Hegde, et al. biotechniques 2000. - [Read A Concise Guide to cDNA Microarray Analysis – II PDF.]

Category: Epigenetics and Methylation Protocols

The pattern and extent of DNA methylation can significantly affect the success of restriction digestions or bacterial transformations. Prokaryotic DNA may be methylated by host restriction/modification systems, while eukaryotic DNA often is methylated i - [Read A practical guide to DNA methylation Promega PDF]

Category: Protein Protocols: Protein Electrophoresis

An excellent guide on the analysis of proteins on SDS-PAGE gels, through staining with coomassie blue dye and western blot analysis. Analysis of Protein Gels (SDS-PAGE). David R. Caprette, Rice University. - [Read Analysis of Protein Gels (SDS-PAGE)]

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Apoptosis Info and Protocols a Promega Guide. - [Read Apoptosis Info and Protocols Promega]

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Cloning Enzymes a Guide Promega. An Enzyme Resource Guide series, highlights those enzymes important in nucleic acid cloning procedures. Promega - [Read Cloning Enzymes a Guide Promega]

Category: Bacterial Protocols

Culture protocols and information for T brucei. Detailed guide to the practice and history ofTrypanosoma brucei cultivation. This covers the culture of bloodstream and procyclic forms in liquid media and on agarose plates. - [Read Culture Protocols and Information for T brucei]

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: Troubleshooting Guides and FAQs - Stem Cells and ES Cells

ES Cell - Stem Cell Troubleshooting Guide. ESGRO - [Read ES Cell - Stem Cell Troubleshooting Guide - ESGRO]

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

ES Cell Culture Protocols - EXCELLENT GUIDE. Harvesting and DNA Preparation, Colony Picking, Electroporation, ES Cell Culture using ESGRO® and Feeder Cell Layer. ESGRO. - [Read ES Cell Culture Protocols - ESGRO]

Category: Animal Research Techniques

Needs of animals, Ventilation, lighting and temperature considerations. Cage size/density. Handling. Blood Collection and guidlines. EXCELLENT GUIDE GC470 ESSENTIALS FOR ANIMAL RESEARCH BRL Univ. Illinois. - [Read Ethics, Guidelines for Animal Research PDF]

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

FISH Guide and Troubleshooting. Includes: Cytogenetic techniques; Slide preparation; When do chromosomes spread; Cell suspensions; G-banding; Cell fixatives; Slide preparation factors. - [Read FISH Guide and Troubleshooting]

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Fixation is to preserve cells and tissue constituents in as close a life-like state as possible and to allow them to undergo further preparative procedures without change. Fixation arrests autolysis and bacterial decomposition and stabilises the cellular and tissue constituents so that they withstand the subsequent stages of tissue processing. Great detailed guide with protocols. Anthony S-Y Leong. - [Read Fixation and Fixatives - A great guide]

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Advantages of Freezing Cell Cultures, Practical Aspects of Cell Freezing, Cryoprotection, Storage Vessels, Labeling and Recordkeeping, Recovery, Managing a Cell Repository, cell selection, Problem Solving Suggestions. Corning. by John A. Ryan - [Read General Guide for Cryogenically Storing Animal Cell Cultures]

Category: Cell Biology and Cell Culture

Guide for generating hybridomas. Eppendorf - [Read Guide for generating hybridomas]

Category: Cell Biology and Cell Culture: Troubleshooting Cell Culture Problems

Guide For Identifying And Correcting Common Cell Growth Problems. Corning. Surface Treatment Process, Problems Related to Technique, Problems Related to Incubators, Problems Related to Culture Media, Problem Solving Suggestions. - [Read Guide For Identifying And Correcting Common Cell Growth Problems]

Category: C. elegans Protocols: C. elegans Genetics Protocols

A guide to genetic mapping in C. elegans. Includes: Introduction and basics; Two-point mapping; Three-point mapping; Deficiency and duplication mapping; Basics of SNP mapping; Mapping dominant mutations; Mapping suppressor/enhance mutations; Mapping of synthetic mutations. - [Read Guide to Genetic Mapping in C. elegans]

Category: Cell Biology and Cell Culture: Cell Culture Techniques

ECACC Handbook of Cell Culture. Fundamental Techniques in Cell Culture: A Labratory Handbook. Sigma Aldrich. Excellent Guide to Cell Culture, aseptic technique, storage of cell lines, subculture of cells. - [Read Handbook of Cell Culture. - Sigma Aldrich.]

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

hES - Human Embryonic Stem Cell Culture Manual. Excellent Manual with Pictures. Troubleshooting guide included. Many Topics Included. ES Cell International Pte Ltd. - [Read Human Embryonic Stem Cell Culture Manual]

Category: Immunohistochemistry Protocols

Immunohistochemistry trouble shooting guide. Includes problem, possible cause and solution. - [Read Immunohistochemistry Trouble Shooting Guide]

Category: Protein Protocols: Immunoprecipitation Protocols: Immunoprecipitation Troubleshooting

Immunoprecipitation Troubleshooting Guide. Stressgen. Problem: Non-specific background, Specific Background, Specific antigen not detected. With Solutions to common IP problems. - [Read Immunoprecipitation Troubleshooting Guide PDF]

Category: Protein Protocols: Immunoprecipitation Protocols: Immunoprecipitation Troubleshooting

Immunoprecipitation TroubleShooting Guide. Sigma Aldrich. Problems: No binding, High background or unwanted proteins precipitate. With Solutions. - [Read Immunoprecipitation TroubleShooting Guide Sigma]

Category: PCR Protocols: Long PCR Protocol. Extended PCR.

Long Template PCR. Troubleshooting Long PCR, Amplification of Long PCR Fragments, Roche Guide. - [Read Long Template PCR. Roche Guide.]

Category: DNA Microarray Protocols

The "7 keys to successful microarray analysis" has been designed to guide researchers step-by-step through the design, implementation, analysis, and publication of a microarray experiment. - [Read Microarray Success]

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Mitogen Activated Protein Kinase Guide. MEK, MEKK, MAPK, MAPKK, antibodies and kits Promega. - [Read Mitogen Activated Protein Kinase Guide]

Category: Molecular Imaging

The Handbook — A Guide to Fluorescent Probes and Labeling Technologies is a comprehensive resource for fluorescence technology and its applications. Newly revised, The Handbook contains detailed information describing the use of more than 3000 Molecular - [Read Molecular Probes Handbook Invitrogen]