gtpÎ³s Protocols

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General Method for Ras, Rac, Cdc42, and Rho Activation Assay - http://www.upstate.com/misc/protocol_detail.q.prot.e.general_method_ras.a.name.e.General_Method_for_Ras_Rac_Cdc42_and_Rho_Activation_Assay

Category: Signalling Signal Transduction Protocols

General protocol for Ras, Rac, Cdc42, and Rho activation assay. Includes: Affinity Precipitation/Immunoblot Protocol, Cell Culture and Extract Preparation (Adherent and Non Adherent cells), GTPÎ³S/GDP Loading for Positive and Negative Controls, Ras, Rac ,Cdc42, and Rho Pull-Down Assay and Western Blot and Detection. - [Read General Method for Ras, Rac, Cdc42, and Rho Activation Assay]

GTPÎ³S-induced Inhibition of Binding - http://www.hopkinsmedicine.org/cellbio/devreotes/gtp.htm

Category: Immunology: Chemotaxis

GTPÎ³S-induced Inhibition of Binding. Chemotaxis assay protocols. Devreotes Lab, John Hopkins Medical Centre. - [Read GTPÎ³S-induced Inhibition of Binding]

GTPÎ³S-Induced Inhibition of cAMP Binding to the cAMP Receptor (cAR1) in Dictyostelium Discoideum - http://www.bio.com/protocolstools/protocol.jhtml?id=p1175

Category: Signalling Signal Transduction Protocols

This protocol assays inhibition of in vivo binding of [3H]-cAMP to cAR1 by GTPÎ³S. Dictyostelium discoideum respond to extracellular cAMP through the cAMP chemoattractant receptor (cAR1). Binding of cAMP to the G protein-coupled cAR1 is inhibited by the GTP analog GTPÎ³S. Protocol includes information on: Solutions used, BioReagents and Chemicals and Protocol Hints. - [Read GTPÎ³S-Induced Inhibition of cAMP Binding to the cAMP Receptor (cAR1) in Dictyostelium Discoideum]