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6-Azauracil Sensitivity Assay for Yeast Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4613

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol describes an assay where it requires growing saturated cultures of yeast, counting, and spotting serial dilutions of yeast on both CSM and CSM + 6AU plates. - [Read 6-Azauracil Sensitivity Assay for Yeast Protocol]

Application of Direct Current Electric Fields to Cells and Tissues in vitro - http://www.natureprotocols.com/2006/12/12/application_of_direct_current.php

Category: Cell Biology and Cell Culture

Protocol applies EFs to cells in vitro but has been modified and to use electrotactic chambers to accommodate cells growing in planar culture or in three-dimensional (3D) gels, en bloc tissue cultures in 3D and possible small embryos, such as that from frog and zebra fish. The EF is applied to the cells or tissues cultured in a customer designed electrotactic chamber via agar salt bridges, Steinbergâ€™s solution and Ag/AgCl electrodes. - [Read Application of Direct Current Electric Fields to Cells and Tissues in vitro]

Baculovirus Growing and Freezing Insect Cells Protocol - http://www.ebioscience.com/ebioscience/appls/BV.htm#C

Category: Cloning Protocols: Baculovirus Protein Expression Protocols

Protocol for Baculovirus growing and freezing insect cells. - [Read Baculovirus Growing and Freezing Insect Cells Protocol]

Baculovirus Protein Expression Protocols - http://www.ebioscience.com/ebioscience/appls/BV.htm

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Baculovirus protein expression protocols. Includes protocols: Cotransfection Protocol; Analysis of nonsecreted protein expression; Growing and Freezing Insect Cells. - [Read Baculovirus Protein Expression Protocols]

Cell Freezing using Liquid Nitrogen N2 - http://www.liv.ac.uk/~giles/methods/cell_freezing.htm

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing gealthy cells (> 95% viable) the protocol obtains a culture 80-90% viable 24 hours after thawing and growing on test vial from step 9. Antisense Research Group - [Read Cell Freezing using Liquid Nitrogen N2]

Culture of Human Prostatic Carcinoma Cell Lines - http://www.celldeath.de/apometh/rpmi.html

Category: Cell Biology and Cell Culture: Cell Line Culture Conditions

Culture of Human Prostatic Carcinoma Cell Lines, Growing and splitting the cells, Preparation of frozen stocks in liquid nitrogen, How to bring frozen cells back into culture, Concentrations of antibiotics for the selection of stable transfectants. LNCa - [Read Culture of Human Prostatic Carcinoma Cell Lines]

Extraction and Solubilization of Total Protein from Microorganisms Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4224

Category: Bacterial Cell Culture Protocols

The growth conditions of microbial cell cultures and the time of sample collection should be optimized and standardized when growing cells for protein extraction. Because cells may excrete proteases and other extracellular enzymes, and compounds in the medium may interfere with extraction, wash cultures with an isotonic buffer, such as PBS or sucrose before solubilization. - [Read Extraction and Solubilization of Total Protein from Microorganisms Protocol]

Glufosinate Ammonium Selection of Transformed Arabidopsis Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4670

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol describes the use of glufosinate ammonium to select transformed Arabidopsis plants. The major advantage of glufosinate ammonium selection is that it can be performed on plants growing in soil and does not require the use of sterile techniques. - [Read Glufosinate Ammonium Selection of Transformed Arabidopsis Protocol]

Growing Adherent Cells Directly on Tissue Dishes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4290

Category: Cell Culture Protocols

For low-resolution work, cells to be used for staining can be grown directly on regular tissue-culture dishes. It is a convenient method that does not require much preparatory work. - [Read Growing Adherent Cells Directly on Tissue Dishes Protocol]

Growing Adherent Cells on Coverslips or Multiwell Slides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4289

Category: Cell Culture Protocols

Glass is an excellent substrate for most tissue-culture-adapted cells and is compatible with all fixing and staining solutions. Glass coverslips in tissue-culture dishes or in 24-well multiwell plates are suitable carriers, as are multiwell slides. For high-resolution studies, choose glass coverslips of the highest available grade; #1 or #1.5 coverslips are the appropriate thickness. - [Read Growing Adherent Cells on Coverslips or Multiwell Slides Protocol]

Growing Bacteriophage M13 in Liquid Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3994

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Most manipulations with M13, including preparations of viral stocks and isolation of single- and double-stranded DNAs, begin with small-scale liquid cultures that are infected with an M13 plaque, picked from an agar plate. - [Read Growing Bacteriophage M13 in Liquid Culture Protocol]

Growing ES Cells with ESGRO - http://www.esgro-lif.com/esgro/protocols.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Growing ES Cells with ESGRO. - [Read Growing ES Cells with ESGRO]

High Throughput and Sensitive Assay Measure Yeast Cell Growth Protocol - http://www.natureprotocols.com/2006/11/22/highthroughput_and_sensitive_a.php

Category: Pharmacology and Toxicology Protocols: Drug Discovery

High-throughput and sensitive assay to measure yeast cell growth: a bench protocol for testing genotoxic agents. Method is highly sensitive, provides quantifiable data and offers high-throughput screening capability. Starting with the treatment of cells with different doses of damaging agents, pre-prepared growing media containing 96-well plates are inoculated and cell population is automatically monitored every 10 min for 48 hours. - [Read High Throughput and Sensitive Assay Measure Yeast Cell Growth Protocol]

Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using bar1 Mutants Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4173

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

It is sometimes desirable to have an entire population of yeast cells that is growing synchronously or is arrested at a unique point in the cell cycle. - [Read Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using bar1 Mutants Protocol]

Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using Low Cell Concentration Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4171

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using Low pH Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4172

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Inducing Yeast Cell Synchrony: Dilution of Stationary-Phase Cultures Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4176

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Inducing Yeast Cell Synchrony: Hydroxyurea Arrest Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4174

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Inducing Yeast Cell Synchrony: Nocodazole Arrest Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4175

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Labeling Monoclonal Antibodies by Biosynthesis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4288

Category: Antibody Protocols: Antibody Labeling Protocols

This method for tagging monoclonal antibodies involves growing hybridomas in the presence of radioactive amino acids. This protocol can be particularly useful when conventional labeling techniques cause the antibody to lose activity. The labeled antibodies that result are essentially identical to the unlabeled antibodies. - [Read Labeling Monoclonal Antibodies by Biosynthesis Protocol]

Liquid Culture of Worms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=22

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Protocol for liquid culture of worms. Includes: superbroth; S- basal; worm plates; GROWING THE BACTERIA (WORM FOOD); GROWING THE WORMS; AFTER THE CULTURE HAS GROWN; PREPARING EGGS TO START SYNCHRONIZED LIQUID CULTURES. - [Read Liquid Culture of Worms Protocol]

Methods for Synchronizing Cells at Specific Stages of the Cell Cycle - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662F47F15A7860600C31BDC6CCF455&objectid=6673FC95E03763BC6E766F163D5338A0

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Exponentially growing cells are asynchronous with respect to the cell cycle stage. Detection of cell cycle-related events is improved by enriching the culture for cells at the stage during which the particular event occurs. Methods for synchronizing cells are provided here, including those based on morphological features of the cell. - [Read Methods for Synchronizing Cells at Specific Stages of the Cell Cycle]

Screening Bacterial Colonies by Hybridization: Intermediate Numbers Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3946

Category: Bacterial Protocols

Bacterial colonies growing on agar plates are transferred en masse to nitrocellulose filters. The spatial arrangement of colonies on the plates is preserved on the filters. After transfer, the filters are processed for hybridization to an appropriate radiolabeled probe while the original (master) plate is incubated for a few hours to allow the bacterial colonies to regrow in their original positions. - [Read Screening Bacterial Colonies by Hybridization: Intermediate Numbers Protocol]

Storage of Bacterial Cultures Growing in Liquid Media Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4452

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

Protocol describes a method for storing bacterial cultures containing glycerol which are growing in liquid media. - [Read Storage of Bacterial Cultures Growing in Liquid Media Protocol]

Storage of Bacterial Cultures Growing on Solid Medium Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4453

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

Protocol describes a method for storing bacterial cultures that are growing on solid media. It is useful for storing copies of cDNA. - [Read Storage of Bacterial Cultures Growing on Solid Medium Protocol]

Articles (1-2 of 2)

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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