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Optimization of Elution Conditions for Dye-Ligand Affinity Chromatography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4211

Category: Chromatography Protocols: Dye Ligand Affinity Chromatography Protocols

Protocol for the optimization of elution condition for dye-ligand affinity chromotography. Elution methods used in dye-ligand affinity chromatography may be either selective or nonselective in nature. Usually, selective elution methods are applied in combination with group-specific adsorbents, such as dye-ligand adsorbents, and nonselective elution methods are used in combination with highly specific adsorbents. - [Read Optimization of Elution Conditions for Dye-Ligand Affinity Chromatography Protocol]

PCR Protocol Buffer Creation - http://www.fhcrc.org/science/labs/soriano/protocols/pcr.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

PCR Protocol Buffer Creation. Based on Jane Gitschier's group.Fred Hutchinson Cancer Research Center. - [Read PCR Protocol Buffer Creation]

PCR Protocol Buffer Creation - http://www.fhcrc.org/science/labs/soriano/protocols/pcr.html

Category: PCR Protocols

PCR Protocol Buffer Creation. Based on Jane Gitschier's group.Fred Hutchinson Cancer Research Center. - [Read PCR Protocol Buffer Creation]

PCR-Based Screening of DNA Libraries Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4129

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

PCR-Based Screening of DNA Libraries Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4129

Category: PCR Protocols

PCR can be used to identify rare DNA sequences in DNA libraries by increasing the abundance of a particular sequence. This is accomplished by subdividing the original library into pools of decreased complexity and screening each pool or group of pools for a given DNA sequence. A pool that contains the desired clone is subsequently subdivided into smaller pools, each of which is screened using the same PCR protocol that was used for the primary screen. - [Read PCR-Based Screening of DNA Libraries Protocol]

PDP-Hydrazide PDF - http://www.piercenet.com/files/0505MR.PDF

Category: Molecular Biology Protocols: Carbohydrate Protocols

PDP-Hydrazide 3-(2-pyridyldithio)propionyl hydrazide is heterobifunctional cleavable crosslinker containing a hydrazide group and pyridyl disulfide residue that react with carbohydrates and sulfhydryls, respectively. Pierce - [Read PDP-Hydrazide PDF]

Phage Media Preparation - http://www.rpgroup.caltech.edu/~paul/protocols/phage/media/

Category: Molecular Biology Protocols: Phage Protocols and Methods

Preparation of liquid medium for phage growth. Rob Phillips Group: Physical Biology of the Cell - [Read Phage Media Preparation]

Phage Protocols - DNA Gels - http://www.rpgroup.caltech.edu/~paul/protocols/phage/lambda/gel/

Category: Molecular Biology Protocols: Phage Protocols and Methods

Phenol/chloroform DNA extraction, running a gel for DNA from Phages. Rob Phillips Group: Physical Biology of the Cell - [Read Phage Protocols - DNA Gels]

Preparation of Electrocompetent E. coli (i.e. DH5 ). PDF. - http://www.its.caltech.edu/%7Ebjorker/Prep_of_electocomp_cells.pdf

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Preparation of Electrocompetent E. coli (i.e. DH5 ). PDF. Bjorkman Group, California I.T. - [Read Preparation of Electrocompetent E. coli (i.e. DH5 ). PDF.]

Protein Expression and Purification Protocol - http://sosnick.uchicago.edu/Protein_Express&Purify.html

Category: Recombinant Protein Protocols

From plasmid to protein using bacterial expression. Transform appropriate DNA plasmid, Make a starter culture for protein expression, bacteria culture for protein expression. Sosnick Group Chicago. - [Read Protein Expression and Purification Protocol]

Restriction Endonuclease Digestion of DNA - http://www.cf.ac.uk/biosi/staff/kille/Methods/Gene/Restriction_Digests.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Restriction Endonuclease Digestion of DNA. Double and Single Digestions. Cardiff Univ. PK group - [Read Restriction Endonuclease Digestion of DNA]

Silver Staining for Nucleolar Organizer Regions - http://www.le.ac.uk/biology/phh4/agnor.htm

Category: Cytogenetics Protocols

Silver Staining for Nucleolar Organizer Regions. Trude Schwarzacher and Molecular Cytogenetics Research Group - [Read Silver Staining for Nucleolar Organizer Regions]

Single tube confirmation PCR protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Single tube confirmation PCR protocol. Saccharomyces Genome Deletion Project. - [Read Single tube confirmation PCR protocol]

Single tube confirmation PCR protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: PCR Protocols

Single tube confirmation PCR protocol. Saccharomyces Genome Deletion Project. - [Read Single tube confirmation PCR protocol]

Single tube confirmation PCR protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: PCR Protocols: Colony PCR

The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformation along with a wild-type control. A series of five different PCR tests are performed on each colony to - [Read Single tube confirmation PCR protocol]

Single Tube Confirmation Yeast PCR Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for single tube confirmation yeast PCR. Includes: Clonal purification; Zymolyase treatment; PCR reactions; PCR conditions; Agarose gel electrophoresis. - [Read Single Tube Confirmation Yeast PCR Protocol]

Subcloning tips and methods. - http://www.its.caltech.edu/%7Ebjorker/Martin_subcloning%20procd.pdf

Category: DNA Protocols: DNA Cloning

Subcloning tips and methods. Very good for all cloning procedures. Bjorkman Group - [Read Subcloning tips and methods.]

Titering & Propagation of Phage - http://www.rpgroup.caltech.edu/~paul/protocols/phage/propagation/

Category: Molecular Biology Protocols: Phage Protocols and Methods

Titering" a phage means measuring the concentration of phage in a given solution. Titering Procedure. Rob Philip's Group. - [Read Titering & Propagation of Phage]

Western Blot Detailed (PDF) - http://www.its.caltech.edu/%7Ebjorker/Western_blot.pdf

Category: Protein Protocols: Western Blot Protocol

Protocol includes cell lysate preparation, SDS-PAGE gels, transfer, and blotting. (Bjorkman Group). - [Read Western Blot Detailed (PDF)]

Western Blot Protocol - http://www.sgul.ac.uk/depts/immunology/~dash/group/western.html

Category: Protein Protocols: Western Blot Protocol

Nice Tips on reducing sample viscosity, buffer preparation, and western blot protocol. Phil Dash. - [Read Western Blot Protocol]

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Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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