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96-Well Confirmation Yeast PCR Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/96_well_confirmation.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for 96-well confirmation Yeast PCR. Includes: Clonal purification; Generate a master plate (96-well format); Making a frozen backup stock; Confirmation PCR for one Row; ORF Specific Confirmation PCR --> "A-B" primers (upstream junction); Transfer template DNA to multiwell PCR plate; Prepare and dispense master mix for A-B PCR. - [Read 96-Well Confirmation Yeast PCR Protocol]

Alternate Sporulation Method for BY4743 Protocol - http://www-sequence.stanford.edu/group/yeast_deletion_project/spo_riles

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for an alternative method for sporulation for BY4743. - [Read Alternate Sporulation Method for BY4743 Protocol]

An Integrative Procedure For Apoptosis Identification And Measurement - http://www.natureprotocols.com/2006/08/03/an_integrative_procedure_for_a.php

Category: Cell Biology and Cell Culture: Apoptosis Protocols: Apoptosis Analysis

An Integrative Procedure for Apoptosis Identification and Measurement. Yingyu Cui Lab/Group: National Key Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences. I have uploaded an integrative procedure through which a relatively satisfactory result can be obtained following a single stage of cell culture and transient cell treatment, then detection with different instruments. This shortens experiment time. - [Read An Integrative Procedure For Apoptosis Identification And Measurement]

Antibody Array Production and Analysis - http://www.stanford.edu/group/robinsonlab/protocols/protocols_tools.htm

Category: Antibody Microarray Protocols

Antibody Array Production and Data Analysis. Probing Antigen Arrays and washing/ Blocking. Great Protocols and information. Brian Kidd. Stanford. - [Read Antibody Array Production and Analysis]

Antibody Purification using Ammonium Sulphate Precipitation - http://www.icp.ucl.ac.be/mexp/vipg_pur.html

Category: Protein Protocols: Antibody Purification Protocols

Antibody Purification using Ammonium Sulphate Precipitation. Viral Immunity and Pathogenesis Group. - [Read Antibody Purification using Ammonium Sulphate Precipitation]

aRNA Labeling and Hybridization Protocol - http://www.stanford.edu/group/sjeffreylab/PDF%20Files/aRNA_labeling.pdf

Category: Microarray Protocols: Microarray Analysis Protocols

Protocol for aRNA labeling and hybridization. - [Read aRNA Labeling and Hybridization Protocol]

Calcium phosphate Tranfection for MDCK cells - http://www.its.caltech.edu/%7Ebjorker/Ca-P_Transfection_MDCK.pdf

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Protocol for cell transfection, clone screening and picking cell clones. Bjorkman Group. California I.T - [Read Calcium phosphate Tranfection for MDCK cells]

Cell Freezing using Liquid Nitrogen N2 - http://www.liv.ac.uk/~giles/methods/cell_freezing.htm

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing gealthy cells (> 95% viable) the protocol obtains a culture 80-90% viable 24 hours after thawing and growing on test vial from step 9. Antisense Research Group - [Read Cell Freezing using Liquid Nitrogen N2]

Cyanine Dye (Maleimide) Protein Labeling Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000150.pdf?pid=PP00000150

Category: Fluorescent Dyes Protocols

Cyanine dye reagents are useful as fluorescent labels for proteins. This protocol has been designed to label the thiol group on cysteine using Cy3 or Cy5 minimal maleimide labeling dyes. - [Read Cyanine Dye (Maleimide) Protein Labeling Protocol]

ELISA for detection of platelet-associated Ig (PAIg) - http://www.icp.ucl.ac.be/mexp/vipg_plelisa.html

Category: ELISA Protocols

Qualitative ELISA (Enzyme-Linked Immunosorbent Assay) used for screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg). Andrei Musaji Viral immunity and pathogenesis group, Universite Catholique de Louvain. - [Read ELISA for detection of platelet-associated Ig (PAIg)]

Erythrophagocytosis Assay - http://www.icp.ucl.ac.be/mexp/vipg_eph.html

Category: Immunology: Macrophage Monocyte Protocols

Erythrophagocytosis Assay. The erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts. Andrei Musaji Viral Immunity and Pathogenesis Group - [Read Erythrophagocytosis Assay]

Fugene Transfection Protocol PDF - http://www.stanford.edu/group/meyerlab/resources/Protocols/Fugene%20Transfection%20Protocol.pdf

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Fugene Transfection Protocol PDF. Meyer Lab Standford. - [Read Fugene Transfection Protocol PDF]

Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols - http://www.fgsc.net/fgn48/Kaminskyj.htm

Category: Fungi Protocols: Aspergillus Protocols

Compendium of protocols for using Aspergillus nidulans in genetic, molecular, and cell biological investigations, originally written for members of my research group. It also summarizes our common growth media and nutritional supplements, many of which originally appeared elsewhere but now are difficult to locate. Includes: Growth and storage of Aspergillus nidulans conidia; Nutritional supplements for our common auxotrophies; Double mutants; Mitotic mapping - assigning genes to chromosomes; etc - [Read Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols]

How to use Alcoy for Linkage Group Assignment - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20alcoy%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use alcoy for linkage group assignment. - [Read How to use Alcoy for Linkage Group Assignment]

In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis - http://web.archive.org/web/20010906052645/http://www.pharma.ethz.ch/bmm/protocols/ingeldig.html

Category: Proteomic Protocols: 2-D Gel Electrophoresis

In gel trypsin digestion for Mass spectrometry. (According to EMBL Protein and Peptide Group, 1997) - [Read In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis]

Introduction of Caged Peptide/Protein into Cells Using Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4659

Category: Microinjection Protocols: Microinjection of Proteins Protocols

Activation and inactivation of proteins using photoactivation of caged peptides or proteins offer insights into cellular dynamics not achievable using genetic means. The ability to selectively alter the activity of a specific protein at a defined time and location inside a cell allows the correlation of changes in protein activity and cellular behavior. A caged compound, peptide, or protein is prepared by covalently linking it to a photolabile, protecting group. - [Read Introduction of Caged Peptide/Protein into Cells Using Microinjection Protocol]

Isolation of proteins from inclusion bodies - http://www.its.caltech.edu/%7Ebjorker/Isolation%20of%20proteins%20from%20.pdf

Category: Recombinant Protein Protocols

Isolation of proteins from inclusion bodies Protcol. Expression of recombinant proteins. Dissolving of recombinant protein. Refolding buffer. BJÃ–RKman group - [Read Isolation of proteins from inclusion bodies]

Lambda plate lysis Protocol - http://www.rpgroup.caltech.edu/~paul/protocols/phage/lambda/plate/

Category: Molecular Biology Protocols: Phage Protocols and Methods: Lambda Phage Protocols

Phage Protocols - Lambda plate lysis. Rob Philip Group. - [Read Lambda plate lysis Protocol]

LB Agar Preparation - http://sosnick.uchicago.edu/LB_agar.html

Category: Buffers Media, and Solutions: Bacterial Solutions

LB Agar Preparation. Sosnick Group. - [Read LB Agar Preparation]

MALDI Sample Preparation - Dried Droplet Method - http://web.archive.org/web/20040523162502/http://www.narrador.embl-heidelberg.de/GroupPages/PageLink/activities/protocols/drieddroplet.html

Category: Mass Spectrometry Protocols

Method for sample preparation for MALDI: dried droplet protocol. EMBL Bioanalytical Research Group. Matthias Wilm . - [Read MALDI Sample Preparation - Dried Droplet Method]

MALDI Sample Preparation - Fast Evaporation Method - http://www.narrador.embl-heidelberg.de/GroupPages/PageLink/activities/protocols/fastevaporation.html

Category: Mass Spectrometry Protocols

Fast Evaporation Method for MALDI sample preparation protocol. Inserting into MALDI mass spectrometer and acquiring a spectrum. EMBL Bioanalytical Research Group. Wilm. - [Read MALDI Sample Preparation - Fast Evaporation Method]

Mass spectrometry compatible silver staining of gels protocol - http://www.narrador.embl-heidelberg.de/GroupPages/PageLink/activities/protocols/silverstain.html

Category: Mass Spectrometry Protocols

Mass spectrometry compatible silver staining of gels protocol. EMBL Bioanalytical Research Group. Wilm. - [Read Mass spectrometry compatible silver staining of gels protocol]

MONOCYTE AND NEUTROPHIL ISOLATION - http://aactg.s-3.com/pub/download/imm/phagocyt.pdf

Category: Immunology: Chemotaxis

MONOCYTE AND NEUTROPHIL ISOLATION. The protocol describes the method used for isolation of monocytes and neutrophils used in both the chemotaxis assay and microbicidal assays. Pediatric AIDS Clinical Trials Group - [Read MONOCYTE AND NEUTROPHIL ISOLATION]

Multiwell Transformation Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/transprot.html

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for multiwell transformation. - [Read Multiwell Transformation Protocol]

Oligonucleotide Details for PCR - http://sequence-www.stanford.edu/group/yeast_deletion_project/Primer_specs.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Information for oligonucleotide details required for PCR. Includes: Primer choice; UPTAG PRIMER; DNTAG PRIMER; UP_45 and DOWN_45 PRIMERS; UP_90 and DOWN_90 PRIMERS. - [Read Oligonucleotide Details for PCR]

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Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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