green Protocols

Category: Cell Organelle Protocols: Mitochondria Protocols

The technique of JC-1 staining has been developed with the intent to detect DY in intact, viable cells. For this purpose JC-1 acts as a marker of mitochondrial activity, since the formation of J-aggregates, which give red emission, is reversible. Cells with high DY are those forming J-aggregates, thus showing high red fluorescence. On the other hand, cells with low DY are those in which JC-1 maintains (or re-acquire) monomeric form, thus showing only green fluorescence. - [Read Analysis of Mitochondrial Membrane Potential with the Sensitive Fluorescent Probe JC-1]

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

Protocol to demonstrate amyloid deposits in tissue sections. When stained with the Congo Red Stain the amyloid, with the aide of polarizing lenses, will birefringe an apple green color.  Under the microscope. - [Read Congo Red Putcher's Modification Amyloid Protocol]

Category: Chromatography Protocols

FPLC Protocol. The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting. For use of individual columns follow the "instructions" (in the green folder) which accompany each one. Sir William Dunn School of Pathology, Oxford University. - [Read FPLC Protocol]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

General PCR Protocol. Sally A. Green - [Read General PCR Protocol]

Category: PCR Protocols

General PCR Protocol. Sally A. Green - [Read General PCR Protocol]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

General PCR Protocol - Sally Green 1996 - [Read General PCR Protocol - Sally Green]

Category: PCR Protocols: Standard PCR Protocol

General PCR Protocol - Sally Green 1996 - [Read General PCR Protocol - Sally Green]

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Data Analysis Protocols

Protocol for measurement of green fluorescent protein expression and DNA content in unfixed cells. - [Read Measurement of Green Fluorescent Protein Expression and DNA Content in Unfixed Cells Protocol]

Category: Neuroscience Protocols: Histology Stains Protocols: Nucleic Acid Stains Protocols

Protocol for methyl green pyronin. - [Read Methyl Green Pyronin Protocol]

Category: Reporter Gene Assays: GFP Assay Protocols

Green fluorescent protein is commonly used to monitor gene expression and protein trafficking within intact cells. The Monster Green® Fluorescent Protein is encoded by an improved synthetic version of the green fluorescent protein gene originally cloned from Montastrea cavernosa (Great Star Coral). - [Read Monster Green® Fluorescent Protein Assay]

Category: Yeast Protocols: Yeast Nucleic Acid Protocols

Flow cytometry is used to analyze the quantity of DNA in cells. Since the DNA content of cells varies through the cell cycle, this information can provide an indication of cell cycle progression. This protocol uses SYTOX Green staining. - [Read Preparation of Yeast Cells for Flow Cytometry Protocol]

Category: Reporter Gene Assays: GFP Assay Protocols

Protocol specifically describes data acquisition for a particular variant of GFP (EGFP) or Oregon Green as a donor fluorophore, but it can be adapted for image acquisition of other chromophore. - [Read Probing Protein Interactions Using GFP and FRET]

Category: Real Time PCR

A detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Wang et al 2003. - [Read Protocol for Real-Time RT-PCR Using SYBR Green]

Category: Signalling Signal Transduction Protocols

CHIESA et al 2001. J Biochem. Calcium-sensitive photoprotein aequorin used to analyse Ca2+ homoeostasis at the subcellular level. GFP chimaeras for studying the recruitment of protein kinase C isoforms and the activity of intracellular proteases. - [Read Recombinant aequorin and green fluorescent protein as valuable tools in the study of cell signalling]

Category: Histology Protocols: Histology Staining Procedures

Acid Fast Stain, Colloidal stain, Copper Stain, Congo Red Stain, Giemsa, Methyl Green Pyronin (MGP) Stain, Oil Red O Stain, many others. Internet Pathology Laboratory for Medical Education, Florida State. - [Read Staining Procedures for Histology]

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Protocol for yeast cell cycle by flow cytometry. Includes procedure for dyes: Propidium Iodide and Sytox Green. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]