great Protocols

Category: Microbiology: Antibiotics Concentration in Media LB

Great PDF file for antibiotics. Stanley Maloy Lab. - [Read Antibiotics, Concentrations and Descriptions PDF]

Category: Antibody Microarray Protocols

Antibody Array Production and Data Analysis. Probing Antigen Arrays and washing/ Blocking. Great Protocols and information. Brian Kidd. Stanford. - [Read Antibody Array Production and Analysis]

Category: Protein Protocols: Protein Extraction From Cells

Cell Lysate Extracts. Great protocols for cell lysis preparation from a variety of cell types. There are numerous methods of cell stimulation and lysis. For a given protein, Upstate’s Laboratories determine the specific treatment upon initial testing of its products. It is important to select the correct cell line, stimulation procedure (if any), and lysis protocol. Upstate. - [Read Cell Lysate Extracts]

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Balancing the tubes in the rotor, labeling tubes, and other safety concerns for centrifuge safety. CR Scientific. - [Read Centrifuge Safety - Great Tips]

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Fixation is to preserve cells and tissue constituents in as close a life-like state as possible and to allow them to undergo further preparative procedures without change. Fixation arrests autolysis and bacterial decomposition and stabilises the cellular and tissue constituents so that they withstand the subsequent stages of tissue processing. Great detailed guide with protocols. Anthony S-Y Leong. - [Read Fixation and Fixatives - A great guide]

Category: Protein Protocols: Immunoprecipitation Protocols

Great Tips on IP - Bart's Cookbook - [Read General Principles of Immunoprecipitation - Great Tips]

Category: E Coli Protocols: E coli Genetics Protocols

Protocol for the generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature sensitive allelic exchange system. Technology requires flanking DNA to be cloned into a temperature sensitive vector but the resulting clone allows great flexibility for further modification of the target sequence. It is therefore highly suited to the study of genes in which several rounds of changes are envisaged. - [Read Generation of Gene Deletions and Gene Replacements in Escherichia coli Protocol]

Category: Histology Protocols

Histotechniques - Manual. Great Histology Protocol Handbook. Includes: Tissue Processing, Specimen Accessioning, Fixation, Fixatives, Tissue Processing, Sectioning, H and E staining, more. - [Read Histotechniques - Manual]

Category: Peptide Protocols: Antibodies from Peptides Protocols

Great resource for information. on making antibodies against peptides - Pierce - [Read Information to Make Antibodies against Peptides - Pierce]

Category: Protein Protocols: Protein Electrophoresis

A great introduction to SDS-PAGE. David R. Caprette, Rice University. - [Read Introduction to SDS-PAGE]

Category: DNA Protocols: DNA Extraction Protocols

Also works for onions. Duration ~40 minutes. DNA Isolation with very easily accessible household solutions. Great for school classes. science-projects.com - [Read Isolation of DNA from Strawberries and Raspberries]

Category: Reporter Gene Assays: GFP Assay Protocols

Green fluorescent protein is commonly used to monitor gene expression and protein trafficking within intact cells. The Monster Green® Fluorescent Protein is encoded by an improved synthetic version of the green fluorescent protein gene originally cloned from Montastrea cavernosa (Great Star Coral). - [Read Monster Green® Fluorescent Protein Assay]

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

Great Procedures. rules for ES cell culture, differentiation of ES cells, ES medium, Thawing, Passaging, Freezing. Mouse embryonic stem (ES) cell culture - basic procedures. Max Planck Institute for Molecular Genetics. Boris Greber. - [Read Mouse embryonic stem (ES) cell culture - basic procedures PDF]

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

A Great PCR and Multiplex Guide. Topics for PCR optimization. Primer conditions, PCR temperatures, etc. Octavian Henegariu. - [Read PCR and Multiplex Guide]

Category: PCR Protocols: PCR Optimization

A Great PCR and Multiplex Guide. Topics for PCR optimization. Primer conditions, PCR temperatures, etc. Octavian Henegariu. - [Read PCR and Multiplex Guide]

Category: Peptide Protocols: Peptide Storage

Great peptide storage information. Lerner. Peptides are provided as lyophilized product that should be stored as dry powder at -20°C to -70°C in a desiccator, if possible. After lyophilization, peptides retain significant amounts of water. Peptides are slowly degraded particularly the cysteine-containing peptides are oxidized over time at -20°C. - [Read Peptide Storage Information]

Category: Proteomic Protocols

Protein Identification Strategy Diagram. A great picture of stregies for protein id. PMSC Toronto - [Read Protein Identification Strategy Diagram]

Category: Protein Protocols: Protein Electrophoresis

SDS-PAGE An Introduction. A great introduction to SDS-PAGE for first time users. - [Read SDS-PAGE An Introduction]

Category: General Research Protocols and Methods: Laboratory Safety

Test Knowing How to Practice Safe Science. Yale University. A great online interactive test for lab safety. - [Read Test Knowing How to Practice Safe Science]

Category: Radioactivity: Liquid Scintillation Counting

The Liquid Scintillation Counting Process. Great Review of the Process and detection procedure of the counter. Dr. Gambhir - [Read The Liquid Scintillation Counting Process]