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Screening of cDNA and genomic libraries. - http://boneslab.chembio.ntnu.no/LambdaScreening.html

Category: RNA Protocols: cDNA Libraries Library

General principles and procedures. Good tips. Atle M. Bones Lab. - [Read Screening of cDNA and genomic libraries.]

Stripping Immunoblots for Reprobing or Storage Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4273

Category: Western Blot Protocols: Immunoblot Stripping Protocols

Protocol describes the practice of stripping immunoblots and reprobing to detect multiple proteins on the same blot is a good method to examine two antigens under identical conditions. - [Read Stripping Immunoblots for Reprobing or Storage Protocol]

Subcloning tips and methods. - http://www.its.caltech.edu/%7Ebjorker/Martin_subcloning%20procd.pdf

Category: DNA Protocols: DNA Cloning

Subcloning tips and methods. Very good for all cloning procedures. Bjorkman Group - [Read Subcloning tips and methods.]

Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3869

Category: PCR Protocols: cDNA Synthesis Protocols

Protocol describes the preparation of subtracted cDNA probes by hybridization to an mRNA driver, followed by purification of the single-stranded radiolabeled cDNA by hydroxyapatite chromatography. Before preparing the probe, it is a good idea to have filters (which contain the cDNA library to be screened) ready to hybridize. - [Read Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer Protocol]

Troubleshooting for PCR and multiplex PCR - http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Troubleshooting

Very Good Troubleshooting for PCR and multiplex PCR Guide. Octavian Henegariu. - [Read Troubleshooting for PCR and multiplex PCR]

Troubleshooting for PCR and multiplex PCR - http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html

Category: PCR Protocols: PCR Troubleshooting

Very Good Troubleshooting for PCR and multiplex PCR Guide. Octavian Henegariu. - [Read Troubleshooting for PCR and multiplex PCR]

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Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Microinjection Pipettes Protocol

Microinjection Pipettes Preparation Protocol.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.