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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

Cap-Switching RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4133

Category: PCR Protocols: cDNA Synthesis Protocols

This method, for the selective amplification of full-length cDNA ends, involves the addition of an adapter during reverse transcription. This method takes advantage of the propensity of Moloney murine leukemia virus reverse transcriptase (MMLV RT) to append two to four cytosines to the 3'-end of newly synthesized cDNA strands. The additional residues are added when the enzyme reaches the 5'-cap structure at the end of the mRNA template. - [Read Cap-Switching RACE Protocol]

CaPO4 Transfection for Chromaffin Cells - http://medicine.ucsd.edu/hypertension/list_of_protocols/15_CaPO4_Transfect_sw.htm

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Transfection of DNA into cells using PIBS, DNA, CaCl2 and Calcium phosphate. Daniel O'Connor, UCSD Chromaffin Cell and Hypertension Research - [Read CaPO4 Transfection for Chromaffin Cells]

Carbohydrate Assays - http://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=13

Category: Molecular Biology Protocols: Carbohydrate Protocols

o determine the relative amounts of LPS carbohydrates present in a given strain. The assay can be done on one set of samples and then scanned at the various wavelengths for reasonable data on the 3 types of sugars. HEXOSE ASSAY, 6-DEOXYHEXOSE ASSAY, HEPTOSE ASSAY. Hancock Laboratory. - [Read Carbohydrate Assays]

Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates - http://www.glycotech.com/protocols/Proto9.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Protocol detects specific cell adhesion to glycolipids resolved on TLC plates. Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates. Ronald L. Schnaar~Professor, Johns Hopkins University Medical School, Baltimore, Maryland. Glycotech. - [Read Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates]

Carboxypeptidase-Y C-terminal sequencing - http://prowl.rockefeller.edu/recipes/cproteas/carboxyy.htm

Category: Mass Spectrometry Protocols

Carboxypeptidase-Y C-terminal sequencing and sequencing ladder protocol method. PROWL - [Read Carboxypeptidase-Y C-terminal sequencing]

Cartilage Staining of Xenopus tropicalis Protocol - http://tropicalis.berkeley.edu/home/gene_expression/cartilage-stain/alcian.html

Category: Xenopus Protocols

Protocol describes the use of a basic water-based dye to stain for acid mucosubstances and acetic mucins located on the cartilage of fixed embryos, permitting the examination of cartilage formation. - [Read Cartilage Staining of Xenopus tropicalis Protocol]

Caspase 8 Assay Protocol - http://www.promega.com/tbs/tb332/tb332.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for caspase 8 assay. Includes: Detection of Caspase-8 Activity Using Purified Caspases; Detection of Caspase-8 Activity in Cell-Based Assays. - [Read Caspase 8 Assay Protocol]

Caspase 9 Assay Protocol - http://www.promega.com/tbs/tb333/tb333.pdf

Category: Pharmacology and Toxicology Protocols: Enzyme Assays Protocols

Protocol for caspase 9 assay. Includes: Detection of Caspase-9 Activity Using Purified Caspases; Detection of Caspase-9 Activity in Cell-Based Assays. - [Read Caspase 9 Assay Protocol]

Caspase Detection Methods for Apoptosis - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/chap11.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Caspase Detection Methods for Apoptosis. Zeuner et al. Purdue Cytometry CD-ROM Series. - [Read Caspase Detection Methods for Apoptosis]

CaspaTag Caspase Activity Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/caspatag.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

This assay is based on carboxyfluorescein labeled fluoromethyl ketone (FMK)-peptide inhibitors of caspases. Includes: Working Dilution of FMK-peptide inhibitors; 1X Working Dilution Wash Buffer; Protocol for Flow Cytometry. - [Read CaspaTag Caspase Activity Protocol]

CAT Assay (Liquid Phase) Protocol - http://snbworld.com/cat2.html

Category: Reporter Gene Assays: Cat Assays Protocols

Protocol describing a CAT assay (liquid phase). - [Read CAT Assay (Liquid Phase) Protocol]

CAT Assay Protocol - http://kinase.uhnres.utoronto.ca/methods/CATassay.html

Category: Reporter Gene Assays: Cat Assays Protocols

Protocol for CAT assay. - [Read CAT Assay Protocol]

CAT Assay Protocols - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/VI.%20Transcription/G.%20CAT%20ASSAY.pdf

Category: Reporter Gene Assays: Cat Assays Protocols

Two protocols describing CAT assays. - [Read CAT Assay Protocols]

CAT.INIST.FR - http://cat.inist.fr/?aModele=afficheN&cpsidt=16477965

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

We have established a high-throughput protocol for mouse genotyping using two robotics workstations: a liquid-handling robot to assemble PCR and a ... - [Read CAT.INIST.FR]

CD90 (Thy-1) Antibody Staining Protocol for Immunohistochemistry - http://www.ihcworld.com/_protocols/antibody_protocols/cd90_pharmingen.htm

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for CD90 (Thy-1) antibody staining for Immunohistochemistry. Includes: Primary Antibody; Antigen Retrieval; Detection Methods; Chromogen Substrate; Counterstain. - [Read CD90 (Thy-1) Antibody Staining Protocol for Immunohistochemistry]

cDNA / AFLP - http://www.dpw.wau.nl/pv/aflp/cDNA-AFLP%20Protocol.htm

Category: Genetics and Genomics: Cancer Genetics Protocols

CDNA/AFLP. A tool for transcriptome analysis. - [Read cDNA / AFLP]

cDNA from Frozen Tissue Sections and LCM - http://dir.nichd.nih.gov/lcm/prep.htm

Category: RNA Protocols: RT-PCR and cDNA synthesis

Method of synthesizing cDNA from Frozen Tissue Sections Protocol. Includes LCM - Laser Capture Microdissection Protocols. NIH - [Read cDNA from Frozen Tissue Sections and LCM]

cDNA Library Construction Lecture - http://wine1.sb.fsu.edu/bch5425/lect27/lect27.htm

Category: RNA Protocols: cDNA Libraries Library

cDNA Library Construction Lecture. Dr.Blaber Lab - [Read cDNA Library Construction Lecture]

cDNA Library Screening Protocol - ZAPII - http://www.people.virginia.edu/~gwl6s/protocols/cdna.html

Category: RNA Protocols: cDNA Libraries Library

cDNA Library Screening Protocol - ZAPII Detailed Protocol. XLI-Blue MRF' and cDNA phage library screening. Dr. Laurie. University of Virginia Health Science Center - [Read cDNA Library Screening Protocol - ZAPII]

cDNA Synthesis and Cloning - http://omrf.ouhsc.edu/~frank/CDNA.html

Category: RNA Protocols: cDNA Libraries Library

Protocol for cDNA synthesis and cloning cDNA into plasmid vector. 1st Strand cDNA Synthesis, and determine the efficiency of first strand cDNA synthesis. Also includes second Strand cDNA Synthesis. Dr.Frank - [Read cDNA Synthesis and Cloning]

cDNA Synthesis and Real-Time PCR Using RNA from Laser-Captured Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4108

Category: PCR Protocols: Real-Time PCR Protocols

Protocol describes the real-time PCR using RNA purified from laser-captured tissues Laser Capture Microdissection (LCM): Preparation and Sectioning of Frozen Tissue Blocks and Purification of RNA from Isolated Cells. - [Read cDNA Synthesis and Real-Time PCR Using RNA from Laser-Captured Cells Protocol]

cDNA Synthesis from MOLT-4 Cells - http://www.musc.edu/BCMB/ceramide/protocols/0043.html

Category: RNA Protocols: RT-PCR and cDNA synthesis

cDNA Synthesis from MOLT-4 Cells. First strand of cDNA synthsis. Cungui Mao - [Read cDNA Synthesis from MOLT-4 Cells]

Celestine Blue Solution Protocol - http://www.nottingham.ac.uk/pathology/protocols/celestine.html

Category: Neuroscience Protocols: Histology Stains Protocols

Protocol for celestine blue solution. - [Read Celestine Blue Solution Protocol]

Cell Adhesion Protocol - http://people.virginia.edu/~gwl6s/protocols/adhesion.html

Category: Cell Biology and Cell Culture

Protocol for cell adhesion. - [Read Cell Adhesion Protocol]

Cell Culture Guidelines - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/CellCultureGuidelines.htm

Category: Cell Biology and Cell Culture: Troubleshooting Cell Culture Problems

Immunology Applications Core Facility, Univ. Chicago - [Read Cell Culture Guidelines]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.