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Genetic Engineering with PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3836

Method describes how to modify the termini of PCR products by introducing restriction sites and other features. To reduce the chance of contamination with exogenous DNAs, prepare and use a special set of reagents and solutions for PCR only. Bake all glassware for 6 hours at 150°C and autoclave all plasticware. - [Read Genetic Engineering with PCR Protocol]

Genetic Engineering with PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3836

Category: PCR Protocols: PCR Optimization

In Situ Hybridization for Epstein Barr Virus-Encoded RNA (EBER) Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/EBER-ISH4doublestain.html

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for in situ hybridization for Epstein Barr virus-encoded RNA (EBER). Includes: Glassware decontamination; Double staining for EBER and a second antibody . - [Read In Situ Hybridization for Epstein Barr Virus-Encoded RNA (EBER) Protocol]

Insect Cell Culture and Baculovirus Infections Protocol - http://omrf.ouhsc.edu/~frank/BACULPRO.html

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Protocol for insect cell culture and baculovirus infections. Includes: Cells, Media, Growth Characteristics, Freezing and Thawing Cells, and Glassware. - [Read Insect Cell Culture and Baculovirus Infections Protocol]

Long PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3841

Category: PCR Protocols: Standard PCR Protocol

Protocol can be used to amplify DNA up to 25 kb in length. To reduce the chance of contamination with exogenous DNAs, prepare and use a special set of reagents and solutions for PCR only. Bake all glassware for 6 hours at 150°C and autoclave all plasticware. - [Read Long PCR Protocol]

Neurospora Methods Protocols - http://www.fgsc.net/methods/stanford.html

Category: Fungi Protocols: Neurospora Protocols

Protocols for Neurospora methods. Includes: Standard strains; Crosses; Minimal medium; Color coding of media; Agar substrate for manipulation and isolation; Stock solutions of supplements; Mating-type tests; Preservation of stocks by silica gel; Cleaning of glassware; Control of mites. - [Read Neurospora Methods Protocols]