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Analysis Of DNA Fragmentation Using The JAM Assay (Subscription Required) - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4432

Category: Cell Biology and Cell Culture: Apoptosis Protocols: Apoptosis Analysis

Analysis of DNA Fragmentation Using the JAM Assay. By Shailaja Kasibhatla et al., The JAM assay is based on labeling nuclear DNA of cycling cells with [3H]thymidine and harvesting samples on glass fiber filters. Apoptosis will generate DNA fragments small enough to pass through the glass fiber filter, resulting in decreased radioactivity of the particular sample. Cell-mediated cytotoxicity or cell killing mediated by cytotoxic T lymphocytes (CTL) can also be measured by this technique. - [Read Analysis Of DNA Fragmentation Using The JAM Assay (Subscription Required)]

Calibration of Micropipette Tips Protocols - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4655

Category: Microinjection Protocols: Microinjection Equipment

This protocol describes an easy method for calibrating micropipette tips that have been pulled in the laboratory. It is essential to estimate the internal diameter of the pulled micropipette tip when adjusting parameters for a new puller or new type of glass tubing. A tip diameter of ~0.3 µm is optimal for the microinjection of mammalian cells in culture (e.g., CHO, PtK1, and COS-7). A 10% increase in diameter increases the delivery rate by more than 30% and can cause cell damage. - [Read Calibration of Micropipette Tips Protocols]

Chambers for Examination of Live Cells under Mechanical Stress Protocol - http://www.cshprotocols.org/cgi/content/extract/2007/3/pdb.ip32

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Manual measurement and manipulation of the cell surface requires access to the cells, usually in an open chamber. Temperature-controlled chambers or stage inserts are preferred for maintaining physiological activity during the experiment. For example, heated culture dishes with coverslip glass bottoms (Bioptechs) permit high-resolution fluorescence microscopy of living cells during force application. - [Read Chambers for Examination of Live Cells under Mechanical Stress Protocol]

Glass Antibody Chip Protocol Nunc - http://www.biokom.com.pl/nunc/news/pdf/Nunc%20MicroArray%20Slides%20-%20glass%20protein%20binding.pdf

Category: Antibody Microarray Protocols

Glass Antibody Chip Microarray Protocol Nunc - [Read Glass Antibody Chip Protocol Nunc]

Glass Milk Agarose Gel DNA Extracton Protocol - http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/glass_milk.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

Isolation of DNA fragments using glass milk (GENE-CLEAN). Glass Milk Agarose Gel DNA Extracton Protocol. Minion Lab, College of Veterinary Medicine at Iowa State University. - [Read Glass Milk Agarose Gel DNA Extracton Protocol]

Growing Adherent Cells on Coverslips or Multiwell Slides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4289

Category: Cell Culture Protocols

Glass is an excellent substrate for most tissue-culture-adapted cells and is compatible with all fixing and staining solutions. Glass coverslips in tissue-culture dishes or in 24-well multiwell plates are suitable carriers, as are multiwell slides. For high-resolution studies, choose glass coverslips of the highest available grade; #1 or #1.5 coverslips are the appropriate thickness. - [Read Growing Adherent Cells on Coverslips or Multiwell Slides Protocol]

Hybridization and Detection Using the HC ExpressArray Kit Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4122

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol describes the direct detection of RNA on DNA microarrays using Hybrid Capture (HC) technology and the HC ExpressArray Kit developed by Diagene. The kit uses a proprietary antibody that binds specifically to RNA:DNA hybrids and a second, fluorescently labeled, antibody that detects the primary antibody. Total RNA is applied directly to a glass-spotted DNA microarray, and stable RNA:DNA hybrids are visualized via a Cy3-labeled secondary antibody. - [Read Hybridization and Detection Using the HC ExpressArray Kit Protocol]

Hybridization to a Scanning Array Made on Glass Protocol - http://fds.oup.com/www.oup.co.uk/pdf/pas/6-5-7.pdf

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol for hybridization to a scanning array made on glass. - [Read Hybridization to a Scanning Array Made on Glass Protocol]

Immunohistochemistry using Anti-Ganglioside Antibodies - http://www.glycotech.com/protocols/Proto2.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Indirect method measuring immunofluorescence coupled to second antibody. Best for membrane antigens in addition to intra- and extracellular antigens, may be applied to frozen tissue sections, to cells in suspension, and to cells attached to glass slides or coverslips. Tadashi Tai~Head, Department of Tumor Immunology, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan - [Read Immunohistochemistry using Anti-Ganglioside Antibodies]

Laboratory Safety Program - http://www.uwm.edu/Dept/EHSRM/LAB/index.html

Category: General Research Protocols and Methods: Laboratory Safety

Laboratory Safety Program - Emergency Preparedness, Autoclave Safety, Biosafety, Carcinogens, Chemical Hygiene Plan, Glass Disposal, Fire Safety, Eyewash and Safety Shower Information, Fume Hood Procedures and Practices. University of Wisconsin - Milwaukee. - [Read Laboratory Safety Program]

Laser Capture Microdissection (LCM) - http://cgap-mf.nih.gov/Protocols/Microdissection/LCM.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

LCM utilizes an infrared laser integrated into a standard microscope. A transparent cap is attached to a thermoplastic transparent membrane which lies directly on the surface of a routinely prepared tissue section on a glass slide. The investigator examines the tissue section microscopically and activates the laser when the desired cells underlie the target. This in turn activates the membrane with subsequent binding and procurement of the cells of interest. - [Read Laser Capture Microdissection (LCM)]

Light Microscopy - Microscopes in Cell Biology - http://www.hei.org/research/aemi/lm.htm

Category: Molecular Imaging: Light Microscopy

Light Microscopy - Microscopes in Cell Biology. House Ear Institute. Fluorescence microscopy, Nomarski differential interference contrast, Comparison between phase contrast and interference contrast optical systems , Interference contrast, Phase contrast, Darkfield illumination, alignment of Kohler illumination system, Protocol for using oil immersion lenses, Use of immersion oil, Calculating the final magnification on the photomicrograph, vibration, The coverslip glass, Photomicroscopy. - [Read Light Microscopy - Microscopes in Cell Biology]

Light Microscopy Techniques - http://www.hei.org/research/aemi/clm.htm

Category: Molecular Imaging: Light Microscopy

Ahmanson Center for Advanced Electron Microscopy and Imaging. Use of Semi-Thin Cryosections for Light Microscopy, Immunolabeling of Cryosections on Glass Slides, Problems with Autofluorescence. House Ear Institute - [Read Light Microscopy Techniques]

Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/1/pdb.prot4448

Category: Gene Delivery Protocols: DNA Nanoparticles Protocols

Lipoplex (cationic liposome-DNA complex) is formed via electrostatic interaction of anionic nucleic acids with cationic liposomes. A thin film of lipids is dried on the bottom of a glass tube and rehydrated in an aqueous solution. The resulting liposome suspension is passed through polycarbonate filters of desired pore size. This protocol also describes the preparation, physical properties, and biological activity of liposome-polycation-DNA (LPD) nanoparticles. - [Read Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery Protocol]

Making Pipettes From Hard Glass Capillaries Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4354

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol describes a method for making pipettes from hard glass capillaries. - [Read Making Pipettes From Hard Glass Capillaries Protocol]

Preparation (Pulling) of Needles for Gene Delivery by Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4651

Category: Microinjection Protocols: Microinjection Equipment

This protocol contains methods for pulling microinjection needles using two different models of pipette pullers. The advantage of pulling needles in the laboratory is that a variety of different needle types can be pulled, depending on the samples and cells being injected. An added advantage is cost; once a pipette puller has been purchased, boxes of glass capillaries are inexpensive compared to premade microinjection needles. - [Read Preparation (Pulling) of Needles for Gene Delivery by Microinjection Protocol]

Preparation of cell lysates from yeast using glass beads vortexing - http://www.embl.de/ExternalInfo/protein_unit/draft_frames/protocol_database/Purification/ec_glass_beads.html

Category: Protein Protocols: Protein Extraction From Cells

Preparation of cell lysates from yeast using glass beads vortexing. Includes lysis buffer recipes. The Protein Expression and Purification Facility, EMBL - [Read Preparation of cell lysates from yeast using glass beads vortexing]

Preparation of Chromosome Spreads - http://www.le.ac.uk/bl/phh4/prochrom.htm

Category: Cytogenetics Protocols

An ideal method of tissue preparation ensures both good specimen morphology and that the target molecules are in the optimum state for probe access and hybridization. DNA:DNA in situ hybridization is usually carried out on chromosome spread preparations where chromosome and nuclei are released from cells and spread on a glass microscope slide. This method yields well separated and enlarged chromosomes with good morphology which can be analyzed in transmitted light or fluorescence microscopes. - [Read Preparation of Chromosome Spreads]

Preparation of Genomic DNA from Yeast Using Glass Beads Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4151

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol describes the preparation of genomic DNA from a 5-ml liquid culture of yeast cells. - [Read Preparation of Genomic DNA from Yeast Using Glass Beads Protocol]

Protein Microarrays from Glass to Paper - http://www.stanford.edu/~btomooka/index.htm

Category: Protein Microarray Protocols

Protein Microarrays from Glass to Paper. Stanford. Brian A. Kidd - [Read Protein Microarrays from Glass to Paper]

Resin Processing of Bone Protocol - http://www.nottingham.ac.uk/pathology/protocols/resbonecut.html

Category: Immunohistochemistry Protocols: Resin Staining Protocols

Protocol for resin processing of bone. Includes: FIXATION; PROCESSING; EMBEDDING; RELEASING BLOCK FROM GLASS CONTAINER; SECTIONING. - [Read Resin Processing of Bone Protocol]

Studying Mitosis in Cultured Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cytogenetics Protocols

Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. Another inexpensive and easy-to-use alternative is to grow cells in a culture dish with a glass bottom. Such dishes are suitable for microinjection experiments. - [Read Studying Mitosis in Cultured Mammalian Cells Protocol]

Studying Mitosis in Cultured Mammalian Cells Prtocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cell Biology and Cell Culture: Cell Cycle Protocols: Mitosis Protocols

Unmasking Hidden Epitopes Using the Pressure Cooker Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4530

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

The principle behind the pressure cooker method described here is to use extended periods of heat to break some of the subcellular structures that block antibody access. This approach is appropriate for handling specimens on glass slides. The major advantages of the pressure cooker method are the ability to handle a large number of slides simultaneously, the convenience of using metal racks, and the avoidance of any hot spots that are found in the microwave. - [Read Unmasking Hidden Epitopes Using the Pressure Cooker Protocol]

Articles (1-7 of 7)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.