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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

Chemical Sequencing Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3804

Category: DNA Protocols: DNA Sequencing Protocols

Protocol for chemical sequencing. - [Read Chemical Sequencing Protocol]

Chemokine and Interleukin Antibody Protocols - http://www.capralogics.com/Protocols.htm

Category: Signalling Signal Transduction Protocols: Chemokine Protocols

Western Blotting chemokine receptors, Immunohistochemistry on Paraffin sections for chemokines. - [Read Chemokine and Interleukin Antibody Protocols]

Chemokine Receptor Expression Assay by Flow Cytometry - http://ubik.microbiol.washington.edu/protocols/facs/chemokinereceptorfacs.htm

Category: Signalling Signal Transduction Protocols: Chemokine Protocols

Chemokine Receptor Expression Assay by Flow Cytometry. Mullins Lab. Reagents, Protocol and detailed procedure. - [Read Chemokine Receptor Expression Assay by Flow Cytometry]

Chemotaxis Assay - http://www.cbrinstitute.org/labs/springer/protocols/melissa_chemotaxis.html

Category: Immunology: Chemotaxis

Chemotaxis Assay, Springer Lab. A chemotaxis assay's function is to assess whether a factor or molecule of interest has chemotactic activity on a motile cell type. Chemotaxis is the ability of a factor to cause the migration of a cell. The chemotactic assay is based on the creation of a chemical gradient of the chemotactic agent which will cause cells to migrate through the gradient towards the chemotactic agent. - [Read Chemotaxis Assay]

Chemotaxis Assay Protocol - http://www.cbrinstitute.org/labs/springer/protocols/melissa_chemotaxis.html

Category: Cell Biology and Cell Culture

This chemotaxis assay protocol is based on the premise of creating a gradient of the chemotactic agent and allowing cells to migrate through a membrane towards the chemotactic agent. A chemotaxis assay can determine whether your protein or small molecule of interest has chemotactic activity on a specific cell type. Chemotaxis is then the ability of a protein to direct the migration of a specific cell. - [Read Chemotaxis Assay Protocol]

Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber) - http://bioprotocol.bio.com/protocolstools/protocol.jhtml?id=p2158

Category: Signalling Signal Transduction Protocols: Chemokine Protocols

Simple apparatus to study chemotaxis of leukocytes or other migratory cells. Protocol for Chemotaxis. BioProtocol - [Read Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)]

Chemotaxis Laboratory Lab - http://dictybase.org/techniques/chemotaxis/Nelson_ChemoLab.pdf

Category: Immunology: Chemotaxis

Chemotaxis Laboratory Lab, Introduction to Dictyostelium and Chemotaxis. - [Read Chemotaxis Laboratory Lab]

Chemotaxis Practical - http://dictybase.org/techniques/chemotaxis/Soldati-Chemotaxis_Protocol.pdf

Category: Immunology: Chemotaxis

Chemotaxis Practical. Thierry Soldati. Department of Biological Sciences Animal and Plant Physiology. The assay in brief. Safety and Good Laboratory Practice: Working in a Cell Biology Laboratory. I-Chemotaxis Assay. Observation of Dictyostelium development stages. - [Read Chemotaxis Practical]

Chemotaxis Practical Answers to Questions - http://dictybase.org/techniques/chemotaxis/Soldati-Chemotaxis_Answers.pdf

Category: Immunology: Chemotaxis

Chemotaxis Practical Answers to Questions. Thierry Soldati - [Read Chemotaxis Practical Answers to Questions]

Chemotaxis Practical, Backstage Preparation - http://dictybase.org/techniques/chemotaxis/Soldati-Chemotaxis_Preparation.pdf

Category: Immunology: Chemotaxis

Chemotaxis Practical, Backstage Preparation. Thierry Soldati - [Read Chemotaxis Practical, Backstage Preparation]

Chemotaxis Protocol - http://dictybase.org/techniques/chemotaxis/Hadwiger_Protocol.pdf

Category: Immunology: Chemotaxis

Chemotaxis Protocol by Jeff Hadwiger. Dictyostelium Chemotaxis to Folate: a Simple Assay, from the Hadwiger Laboratory. Detailed Protocol. - [Read Chemotaxis Protocol]

Chemotaxis Under Agar Assay - http://dictybase.org/techniques/chemotaxis/Knecht_UnderagarAssay.pdf

Category: Immunology: Chemotaxis

Chemotaxis Under Agar Assay by David Knecht. - [Read Chemotaxis Under Agar Assay]

CHIFF preparation - http://www.hopkinsmedicine.org/cellbio/devreotes/chiff.htm

Category: Immunology: Chemotaxis

CHIFF preparation. Chemotaxis Assay protocol. Devreotes Lab, John Hopkins Medical Centre. - [Read CHIFF preparation]

ChIP Assay Protocol PDF - http://www.bcm.edu/rosenlab/protocols/ChIP.pdf

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Formaldehyde cross-linking and chromatin immunoprecipitation assays of tissue culture cells, Based on Boyd and Farnham. Michelle Kallesen, Rosen Lab. - [Read ChIP Assay Protocol PDF]

ChIP on ChIP Protocol PDF - http://genomecenter.ucdavis.edu/farnham/farnham/protocols/MOJO%20Dec%202003%20ChIP-chip%20protocol.pdf

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Method to identify genomic targets of DNA-binding factors. Chromatin immunoprecipitation (ChIP) assay on high-throughput microarray based methods for discovering genomic regions occupied by human DNA-binding proteins. Oberley and Farnham. - [Read ChIP on ChIP Protocol PDF]

ChIP ping for Dummies - http://mcardle.oncology.wisc.edu/sugden/Protocols/html%20files/ChIPping%20for%20Dummies.htm

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Very nice and detailed ChIP assay protocol procedure. Blocking Staph A cells, Crosslinking cells, Chromatin preparation from cell pellets, sonication, blocking. Sugden Laboratory - [Read ChIP ping for Dummies]

ChIP Protocol - http://www.genetics.ucla.edu/labs/fan/Protocols_ChIP.htm

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

ChIP Protocol. Fan Lab UCLA. - [Read ChIP Protocol]

ChIP Tips and FAQs - http://www.abcam.com/index.html?rid=310

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

ChIP Tips and FAQs. AbCam. - [Read ChIP Tips and FAQs]

ChIP TIPS PDF - http://www.upstate.com/img/pdf/ChIP_Protocol_thin_v2.pdf

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Prepare Chromatin, Immunoprecipitate, Wash, Elute, IP Procedure. UpState. - [Read ChIP TIPS PDF]

Chlorophyll Content Protocol - http://homepages.gac.edu/~cellab/chpts/chpt8/ex8-2.html

Category: Plant Biology Protocols: Photosynthesis and Respiration Protocols

Protocol describing how to calculate chlorophyll content. - [Read Chlorophyll Content Protocol]

Chloroplast DNA Isolation Protocol - http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=142&criteria=

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol for chloroplast DNA isolation. - [Read Chloroplast DNA Isolation Protocol]

Chloroplast Isolation DNA Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplchl.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol for the isolation of DNA from the chloroplast. - [Read Chloroplast Isolation DNA Protocol]

CHO Centrosome Prep Protocol - http://mitchison.med.harvard.edu/protocols/mt1.html

Category: Cell Organelle Protocols: Nucleus Protocols

The key step is the lysis which solubilizes centrosomes away from nuclei by very low ionic strength lysis after treatment of cells with nocodazole and cytochalasin B. The released centrosomes are then centrifuged onto a Ficoll cushion (to avoid pelleting) and the interface between the lysate and the Ficoll is collected and the centrosomes are concentrated on a sucrose gradient. Fractions are assayed by spindown and double IF with 5051 serum and anti-tubulin and the pooled fractions are frozen... - [Read CHO Centrosome Prep Protocol]

Choosing the Right Labeling Method for your Hybridization Experiment - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_15-18.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

Choosing the right labeling method for your hybridization experiment. Includes: Homogeneous labeling methods for DNA; Homogeneous labeling methods for RNA; Stability of probe-target interaction; Nonradioactive labeling of oligonucleotides; Double-stranded versus single-stranded probes. - [Read Choosing the Right Labeling Method for your Hybridization Experiment]

Chromatin Assembly on Template DNA with Transcription Factors and Drosophila S-190 Chromatin Assembl - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3D3APITJJIWFIM1R3FQLMCFEWHUWBNSIV0?id=p9061

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol described here produces chromatin with regularly spaced nucleosomes having physiological nucleosome repeat lengths; something that can be difficult to achieve with purified components. In addition, chromatin assembled with the S-190 Chromatin Assembly Extract contains the ATP-dependent chromatin remodeling factors necessary for efficient transcription. - [Read Chromatin Assembly on Template DNA with Transcription Factors and Drosophila S-190 Chromatin Assembl]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.