Universal Mouse Genotyping Protocol (downloadable pdf click here). This protocol is designed to detect sequences in the murine genome by polymerase chain ... - [Read Universal Mouse Genotyping Protocol]
This protocol is designed to detect sequences in the murine genome by polymerase chain reaction amplification, and is adapted from Stratman and Simon (Transgenic Res. 12, 521-522 (2003)).For those familiar with PCR genotyping, this method differs from the typical protocol by utilizing a unique enzyme (Klentaq), 30mer primers, and a 68° annealing temperature. - [Read Universal Mouse Genotyping Protocol]